Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TLL_RS00595 Genome accession   NC_004113
Coordinates   97701..98798 (-) Length   365 a.a.
NCBI ID   WP_011055976.1    Uniprot ID   Q8DMJ5
Organism   Thermosynechococcus vestitus BP-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 92701..103798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TLL_RS00570 (tlr0115) - 93876..95027 (+) 1152 WP_172598138.1 cysteine desulfurase family protein -
  TLL_RS00575 (tlr0116) - 95342..95677 (+) 336 WP_011055971.1 hypothetical protein -
  TLL_RS00580 (tsl0117) - 95698..95970 (-) 273 WP_011055972.1 DUF3143 domain-containing protein -
  TLL_RS00585 (tll0118) - 95967..96440 (-) 474 WP_011055973.1 J domain-containing protein -
  TLL_RS13410 - 96449..97670 (-) 1222 Protein_117 type II secretion system F family protein -
  TLL_RS00595 (tll0121) pilT 97701..98798 (-) 1098 WP_011055976.1 type IV pilus twitching motility protein PilT Machinery gene
  TLL_RS00600 (tll0122) pilB/pilB1 98868..100892 (-) 2025 WP_011055977.1 GspE/PulE family protein Machinery gene
  TLL_RS00605 (tsr0123) - 101154..101378 (+) 225 WP_164920641.1 hypothetical protein -
  TLL_RS00610 (tsr0124) - 101379..101576 (+) 198 WP_011055979.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  TLL_RS00615 (tlr0125) - 101540..103114 (+) 1575 WP_164920642.1 right-handed parallel beta-helix repeat-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40410.55 Da        Isoelectric Point: 6.7579

>NTDB_id=22228 TLL_RS00595 WP_011055976.1 97701..98798(-) (pilT) [Thermosynechococcus vestitus BP-1]
MELMIEDLMEQVVANGGSDLHISAGIPPYVRISGKLTPMDYEPLTPEQCQRLIFSMLNNTQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMSERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGIITYEAAMAKSSRQDELQRLIGSGTPAAVAR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=22228 TLL_RS00595 WP_011055976.1 97701..98798(-) (pilT) [Thermosynechococcus vestitus BP-1]
ATGGAGTTGATGATTGAGGATCTCATGGAGCAGGTCGTGGCCAACGGTGGTTCAGACTTGCACATTTCAGCAGGTATCCC
CCCCTACGTACGCATCAGCGGTAAATTAACCCCAATGGATTACGAGCCCCTTACCCCAGAGCAATGCCAGCGCCTGATCT
TCAGTATGCTCAACAATACCCAACGCAAGCACCTAGAGCAAAACTGGGAGTTGGACTGCTCCTATGGCGTGCGGGGTTTA
GCCCGGTTCCGCGTCAATGTCTATAAAGATCGGGGGACCTATGCCGCTTGCTTGCGGGCTTTGAGTTCCAAAATTCCCAC
GTTTGAGCAGTTGGGCTTGCCCAACATTGTTCGCGAAATGAGTGAGCGGCCACGGGGCTTGATTCTGGTGACTGGGCCGA
CGGGATCAGGGAAAACCACAACCTTGGCGGCAATGATTGACCTGATCAACAAAACCCGCGCTGAGCATATTCTGACGATT
GAAGACCCGATTGAGTTTGTCTATGAACCAATCAAGAGCCTGATCCACCAGCGGCAGGTGGGGGAAGACACCAAGAGTTT
TGCCAATGCCCTACGGGCAGCGCTGCGGGAAGACCCCGACATTATCCTCGTGGGTGAGATGCGCGACTTGGAAACGATTC
AGCTTGCCATCACAGCAGCGGAAACAGGTCACTTGGTCTTTGGTACGTTGCACACCAGTTCAGCAGCACAAACCGTTGAC
CGCATGGTGGATGTGTTTCCCCCTGAGCAGCAACAGCAAATTCGCGTCCAGTTGTCCAACTCCTTGGTAGCCGTCTTTAG
CCAGACACTGGTTCCGAAGAAAAATCCCAAGCCCGGTGAATTTGGACGGATTATGGCGCAAGAGATCATGGTGGTTACCC
CTGCTATCTCCAACCTGATTCGCGAAGGCAAGACGTCACAGATTTATTCGGCAATTCAAACGGGTGGCAAGCTGGGGATG
CAAACTCTTGAAAAGGTGCTGGCCGATTATTATCGTGCCGGCATCATCACCTATGAAGCAGCAATGGCCAAGTCTTCGCG
TCAGGACGAGCTGCAACGTCTTATTGGTAGCGGTACACCGGCGGCAGTAGCACGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DMJ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.132

93.425

0.515
  pilT Legionella pneumophila strain ERS1305867

54.203

94.521

0.512
  pilT Legionella pneumophila strain Lp02

54.203

94.521

0.512
  pilT Acinetobacter baylyi ADP1

54.867

92.877

0.51
  pilT Pseudomonas stutzeri DSM 10701

53.644

93.973

0.504
  pilT Acinetobacter baumannii strain A118

53.412

92.329

0.493
  pilT Acinetobacter nosocomialis M2

53.412

92.329

0.493
  pilT Acinetobacter baumannii D1279779

53.412

92.329

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.286

95.89

0.482
  pilT Neisseria gonorrhoeae MS11

49.57

95.616

0.474
  pilT Neisseria meningitidis 8013

49.57

95.616

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.588

93.151

0.471
  pilT Vibrio cholerae strain A1552

50.588

93.151

0.471
  pilU Pseudomonas stutzeri DSM 10701

39.155

97.26

0.381
  pilU Acinetobacter baylyi ADP1

38.109

95.616

0.364

Multiple sequence alignment