Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A8H26_RS00115 Genome accession   NZ_CP020388
Coordinates   23112..24092 (-) Length   326 a.a.
NCBI ID   WP_048254059.1    Uniprot ID   A0AAW8HTD0
Organism   Pluralibacter gergoviae strain FDAARGOS_186     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18112..29092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8H26_RS00085 (A8H26_00085) endA 18461..19198 (+) 738 WP_043082083.1 deoxyribonuclease I -
  A8H26_RS00090 (A8H26_00090) rsmE 19219..19950 (+) 732 WP_043082082.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A8H26_RS00095 (A8H26_00095) gshB 19973..20920 (+) 948 WP_043082081.1 glutathione synthase -
  A8H26_RS00100 (A8H26_00100) - 21019..21582 (+) 564 WP_045287471.1 YqgE/AlgH family protein -
  A8H26_RS00105 (A8H26_00105) ruvX 21582..21998 (+) 417 WP_048254060.1 Holliday junction resolvase RuvX -
  A8H26_RS00110 (A8H26_00110) - 22035..22688 (-) 654 WP_043082078.1 LuxR C-terminal-related transcriptional regulator -
  A8H26_RS00115 (A8H26_00115) pilT 23112..24092 (-) 981 WP_048254059.1 type IV pilus twitching motility protein PilT Machinery gene
  A8H26_RS00120 (A8H26_00120) - 24107..24811 (+) 705 WP_048254058.1 YggS family pyridoxal phosphate-dependent enzyme -
  A8H26_RS00125 (A8H26_00125) - 24833..25399 (+) 567 WP_043082075.1 YggT family protein -
  A8H26_RS00130 (A8H26_00130) yggU 25396..25707 (+) 312 WP_043082074.1 DUF167 family protein YggU -
  A8H26_RS00135 (A8H26_00135) - 25695..26282 (+) 588 WP_048254057.1 XTP/dITP diphosphatase -
  A8H26_RS00140 (A8H26_00140) hemW 26282..27421 (+) 1140 WP_048254056.1 radical SAM family heme chaperone HemW -
  A8H26_RS00145 (A8H26_00145) - 27470..27817 (-) 348 WP_045287464.1 hypothetical protein -
  A8H26_RS00150 (A8H26_00150) ansB 28000..29046 (-) 1047 WP_048254055.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35526.84 Da        Isoelectric Point: 7.2385

>NTDB_id=222051 A8H26_RS00115 WP_048254059.1 23112..24092(-) (pilT) [Pluralibacter gergoviae strain FDAARGOS_186]
MDIEEIVALSVKHNVSDLHLCSAGQVRWRRRGSLERAPFPAPDLQLLLARWLGEAGVQEWLLQRQCDFSVETAGGRRLRA
SAFQHIRGCSLVLRLLPDACPQLSELWAPQALGELLHEESGLILVTGATGSGKSTTLAAMVEHLNRQRDGHILTLEDPVE
FIHRSARCLIQQREVGAHCASFADALRVALRQDPDVILIGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLLDA
FPAGEKEQVRGQLAGSLCAVLAQKLLPDKLGGRVALYELLVNTPAAANLIREGKSHQLPGIMQTGQQAGMLTFSQSFQQR
QAAGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=222051 A8H26_RS00115 WP_048254059.1 23112..24092(-) (pilT) [Pluralibacter gergoviae strain FDAARGOS_186]
ATGGATATTGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCGGGCAGGTGCG
ATGGCGGCGGCGGGGGAGCCTCGAGCGGGCGCCATTCCCGGCGCCGGATCTGCAGCTCCTGCTGGCCCGCTGGCTCGGAG
AGGCGGGGGTACAGGAGTGGCTGCTGCAGCGGCAGTGCGACTTTTCCGTCGAAACGGCGGGCGGCAGGCGGCTGCGCGCC
AGCGCTTTCCAGCATATTCGCGGCTGCTCGTTGGTGCTGCGCCTGCTGCCCGACGCGTGCCCACAGCTCTCTGAACTGTG
GGCGCCGCAGGCCCTTGGCGAGCTGCTCCACGAGGAGAGCGGGCTTATCCTCGTCACCGGAGCGACCGGCAGCGGCAAGT
CCACCACGCTTGCCGCGATGGTGGAACACCTCAACCGGCAGCGGGACGGGCATATTCTGACCCTGGAAGACCCGGTAGAG
TTTATTCACCGCAGCGCCCGCTGCCTGATCCAGCAGCGGGAGGTCGGCGCCCACTGCGCCTCGTTTGCGGACGCGCTGCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGATTGGCGAACTGCGCGACAGCGAGACTATTCGCCTGGCGCTGACCG
CTGCGGAGACCGGGCACCTGGTTCTGGCGACGCTGCACACCCGGGGCGCGGCCCAGGCCGTTGAGCGGCTGCTGGACGCC
TTCCCCGCCGGCGAAAAAGAGCAGGTGCGCGGCCAGCTGGCGGGCAGCCTGTGCGCGGTGCTGGCCCAGAAGCTGCTGCC
AGATAAGCTGGGCGGGCGGGTGGCGCTGTACGAACTGCTGGTAAATACGCCCGCCGCCGCCAACCTGATCCGCGAGGGCA
AGAGCCATCAGCTGCCGGGCATCATGCAGACCGGGCAGCAGGCGGGCATGCTGACCTTCAGCCAGAGTTTTCAGCAGCGG
CAGGCCGCCGGGCGGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

48.137

98.773

0.475
  pilT Neisseria gonorrhoeae MS11

47.826

98.773

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Acinetobacter nosocomialis M2

46.729

98.466

0.46
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

41.925

98.773

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.318

98.466

0.377

Multiple sequence alignment