Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B5S52_RS04415 Genome accession   NZ_CP020350
Coordinates   955650..956666 (+) Length   338 a.a.
NCBI ID   WP_080163331.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain SX309     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 950650..961666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5S52_RS04385 (B5S52_04385) hemW 951364..952506 (-) 1143 WP_040044026.1 radical SAM family heme chaperone HemW -
  B5S52_RS04390 (B5S52_04390) rdgB 952499..953092 (-) 594 WP_080163327.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  B5S52_RS04395 (B5S52_04395) yggU 953110..953400 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  B5S52_RS04400 (B5S52_04400) - 953397..953951 (-) 555 WP_010279107.1 YggT family protein -
  B5S52_RS04405 (B5S52_04405) proC 954000..954821 (-) 822 WP_080164264.1 pyrroline-5-carboxylate reductase -
  B5S52_RS04410 (B5S52_04410) - 954916..955629 (-) 714 WP_080163329.1 YggS family pyridoxal phosphate-dependent enzyme -
  B5S52_RS04415 (B5S52_04415) pilT 955650..956666 (+) 1017 WP_080163331.1 type IV pilus twitching motility protein PilT Machinery gene
  B5S52_RS04420 (B5S52_04420) - 956635..958467 (-) 1833 WP_080163333.1 sensor domain-containing diguanylate cyclase -
  B5S52_RS04425 (B5S52_04425) - 958994..960565 (-) 1572 WP_039526637.1 methyl-accepting chemotaxis protein -
  B5S52_RS04430 (B5S52_04430) nsrR 960909..961334 (+) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36676.87 Da        Isoelectric Point: 6.6533

>NTDB_id=221556 B5S52_RS04415 WP_080163331.1 955650..956666(+) (pilT) [Pectobacterium brasiliense strain SX309]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLAALQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDNSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=221556 B5S52_RS04415 WP_080163331.1 955650..956666(+) (pilT) [Pectobacterium brasiliense strain SX309]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCCGCTCAGAGAGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGGGCATTAATGCTGCCCGATGGGCAA
CGCCTGCGGGTGAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATTCCGTCCGCTCAGCCTTCATT
GGCGGCGCTACAGGCACCACCCGTTCTTTCGACGCTGCTGGAGAAGCCGAATGGACTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAACAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTCATCCATGCCAGCCGACGTTGTCTGATTCAACAGCGGGAGATTGGTGCGCACAGTGCGTCCTTTGC
GCAGGCTCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTCGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGTTTACAGGCCGTGGTGACGCA
AAGGCTGTTGCCCGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCGGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.64

97.929

0.476
  pilT Legionella pneumophila strain Lp02

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.94

98.521

0.393
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367

Multiple sequence alignment