Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TTE_RS06010 Genome accession   NC_003869
Coordinates   1263431..1264480 (+) Length   349 a.a.
NCBI ID   WP_011025582.1    Uniprot ID   Q8RAG0
Organism   Caldanaerobacter subterraneus subsp. tengcongensis MB4     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1258431..1269480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TTE_RS05990 (TTE1259) sigK 1259648..1260343 (+) 696 WP_011025579.1 RNA polymerase sporulation sigma factor SigK -
  TTE_RS05995 (TTE1260) - 1260405..1260905 (+) 501 WP_011025580.1 YqeG family HAD IIIA-type phosphatase -
  TTE_RS06000 (TTE1261) aroE 1260895..1261737 (+) 843 WP_009610692.1 shikimate dehydrogenase -
  TTE_RS06005 (TTE1262) gspE 1261755..1263425 (+) 1671 WP_011025581.1 type II secretion system ATPase GspE -
  TTE_RS06010 (TTE1263) pilT 1263431..1264480 (+) 1050 WP_011025582.1 type IV pilus twitching motility protein PilT Machinery gene
  TTE_RS06015 (TTE1264) - 1264491..1265702 (+) 1212 WP_009610693.1 type II secretion system F family protein -
  TTE_RS06020 (TTE1265) - 1265730..1266056 (+) 327 WP_011025583.1 competence type IV pilus major pilin ComGC -
  TTE_RS06025 (TTE1266) - 1266112..1266870 (+) 759 WP_011025584.1 prepilin peptidase -
  TTE_RS06030 (TTE1267) - 1266875..1267324 (+) 450 WP_011025585.1 hypothetical protein -
  TTE_RS06035 (TTE1268) - 1267336..1268001 (+) 666 WP_041587318.1 type IV pilus modification PilV family protein -
  TTE_RS06040 (TTE1269) - 1267992..1268486 (+) 495 WP_011025587.1 PilW family protein -
  TTE_RS06045 (TTE1270) - 1268476..1269006 (+) 531 WP_011025588.1 PilX N-terminal domain-containing pilus assembly protein -
  TTE_RS06050 (TTE1271) - 1269022..1269282 (+) 261 WP_011025589.1 late competence development ComFB family protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38559.81 Da        Isoelectric Point: 6.7741

>NTDB_id=21975 TTE_RS06010 WP_011025582.1 1263431..1264480(+) (pilT) [Caldanaerobacter subterraneus subsp. tengcongensis MB4]
METIELLKLVVEKKASDLHITVGVPPVVRINGYLQKLEGEPFTPKDTEEIVKDLLTSEQLKKLEENGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPLILKDLALKTRGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDATSFASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIRIQLSNVLEGIISQQLIPKKDGSGRVVATEVMIATPAIRNLIREGKTYQIQSAVQTGGKFGMITMDMS
ILHLLKSGVISLEDALTYCVDQESFSRMI

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=21975 TTE_RS06010 WP_011025582.1 1263431..1264480(+) (pilT) [Caldanaerobacter subterraneus subsp. tengcongensis MB4]
ATGGAGACCATTGAACTTTTGAAATTGGTTGTGGAGAAAAAAGCTTCAGATTTACACATAACAGTAGGAGTTCCACCTGT
TGTAAGAATTAACGGCTATCTTCAAAAATTAGAAGGAGAGCCTTTTACACCTAAAGATACAGAGGAGATAGTCAAAGACC
TACTTACGAGCGAGCAGCTAAAAAAGCTGGAGGAAAATGGGGATATTGACCTTTCCTATTCTGTGACAGGTTTGGGAAGG
TTTAGAATAAATGTGTACAAGCAAAGAGGAAGTTACAGCCTTGCCATAAGGTCTGTTGCCTTGAGAATACCTACTATAGA
AGAGCTGGGACTTCCACTCATACTTAAAGATTTGGCTTTGAAAACGAGGGGATTGATACTTGTTACAGGACCTACGGGAA
GTGGGAAGTCCACCACACTGGCGGCTATGGTGGACTGGATAAATTCTAAAAGGACTTGTCACATTCTTACATTGGAGGAC
CCTATAGAGTATTTGCACAAGCACAATAAAAGCATAGTAAATCAAAGAGAAATTGGGCACGATGCTACATCCTTTGCATC
GGCTTTAAGAGCTGCATTAAGAGAAGACCCGGATGTGATACTTGTCGGTGAGATGAGGGATTTGGAGACAATACAGATTG
CAATAACTGCTGCCGAGACAGGCCATCTTGTTCTTTCCACTCTTCACACAATAGGAGCTGTAAAAACGATTGATAGGATT
ATTGATGTTTTTCCGCCTCATCAGCAACAGCAAATAAGGATACAGCTTTCCAACGTATTAGAAGGAATCATATCTCAGCA
GCTTATTCCTAAAAAAGACGGAAGCGGAAGAGTTGTTGCCACAGAAGTGATGATTGCAACCCCTGCTATAAGGAATCTCA
TAAGGGAAGGGAAGACTTACCAAATACAGTCGGCAGTTCAAACAGGTGGTAAATTTGGAATGATTACTATGGACATGTCT
ATTTTGCACCTTTTAAAATCAGGGGTTATCTCTTTGGAAGATGCTTTAACTTACTGCGTTGACCAGGAGAGCTTTTCACG
CATGATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8RAG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

50.725

98.854

0.501
  pilT Acinetobacter baylyi ADP1

50.725

98.854

0.501
  pilT Acinetobacter nosocomialis M2

50.725

98.854

0.501
  pilT Acinetobacter baumannii strain A118

50.725

98.854

0.501
  pilT Pseudomonas aeruginosa PAK

50.145

98.854

0.496
  pilT Legionella pneumophila strain Lp02

49.855

98.854

0.493
  pilT Legionella pneumophila strain ERS1305867

49.855

98.854

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.599

95.702

0.484
  pilT Vibrio cholerae strain A1552

50.599

95.702

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.128

98.567

0.484
  pilT Pseudomonas stutzeri DSM 10701

48.406

98.854

0.479
  pilT Neisseria meningitidis 8013

46.821

99.14

0.464
  pilT Neisseria gonorrhoeae MS11

46.532

99.14

0.461
  pilU Pseudomonas stutzeri DSM 10701

43.266

100

0.433
  pilU Vibrio cholerae strain A1552

41.369

96.275

0.398
  pilU Acinetobacter baylyi ADP1

40.294

97.421

0.393

Multiple sequence alignment