Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B2G73_RS22310 Genome accession   NZ_CP019986
Coordinates   4522865..4523845 (+) Length   326 a.a.
NCBI ID   WP_079226003.1    Uniprot ID   -
Organism   Citrobacter werkmanii strain BF-6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4517865..4528845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2G73_RS22280 ansB 4518378..4519424 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  B2G73_RS22285 hemW 4519541..4520677 (-) 1137 WP_042310949.1 radical SAM family heme chaperone HemW -
  B2G73_RS22290 - 4520670..4521263 (-) 594 WP_038633614.1 XTP/dITP diphosphatase -
  B2G73_RS22295 yggU 4521271..4521561 (-) 291 WP_042310947.1 DUF167 family protein YggU -
  B2G73_RS22300 - 4521558..4522124 (-) 567 WP_038633609.1 YggT family protein -
  B2G73_RS22305 - 4522143..4522847 (-) 705 WP_079226002.1 YggS family pyridoxal phosphate-dependent enzyme -
  B2G73_RS22310 pilT 4522865..4523845 (+) 981 WP_079226003.1 type IV pilus twitching motility protein PilT Machinery gene
  B2G73_RS22315 ruvX 4523842..4524258 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  B2G73_RS22320 - 4524258..4524821 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  B2G73_RS22325 gshB 4524933..4525880 (-) 948 WP_079226004.1 glutathione synthase -
  B2G73_RS22330 rsmE 4525893..4526624 (-) 732 WP_042310938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  B2G73_RS22335 endA 4526699..4527406 (-) 708 WP_079226005.1 deoxyribonuclease I -
  B2G73_RS22340 - 4527501..4527998 (-) 498 WP_042310936.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35890.08 Da        Isoelectric Point: 6.7137

>NTDB_id=219608 B2G73_RS22310 WP_079226003.1 4522865..4523845(+) (pilT) [Citrobacter werkmanii strain BF-6]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIHGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLTNSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALAELLHSENGLILVTGATGSGKSTTLAAMVSYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLAQKLEQDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGIMQTGQQTGMQTFTQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=219608 B2G73_RS22310 WP_079226003.1 4522865..4523845(+) (pilT) [Citrobacter werkmanii strain BF-6]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACATGGAAATGTCGAAATCGCACCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGACGAACTCACGGCGTCTGCGCGCG
AGCGCATTTGTTCATCAGCAGGGAATCTCTCTGGCGCTAAGGCTGTTGCCGCTTGATTGCCCATGTTTAGATGATCTCCA
GACTCCCGCCGCCTTAGCTGAGCTGCTGCATAGCGAAAATGGATTAATTCTGGTGACAGGCGCCACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGTCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAACGTTGTCTCATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTGCGCGAAGATCCTGACGTCATTCTGCTGGGAGAGCTGCGCGATGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACATTACACACGCGAGGGGCGGCGCAGGCCATTGCACGGCTGGTGGATACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTGCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGGCGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTCGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCATAATGCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAGAGCCAGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

46.226

97.546

0.451
  pilT Legionella pneumophila strain Lp02

46.226

97.546

0.451
  pilT Pseudomonas aeruginosa PAK

44.343

100

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

40.323

95.092

0.383
  pilU Acinetobacter baylyi ADP1

37.963

99.387

0.377

Multiple sequence alignment