Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   STM3099 Genome accession   NC_003197
Coordinates   3259953..3260933 (-) Length   326 a.a.
NCBI ID   NP_462015.1    Uniprot ID   Q8ZM48
Organism   Salmonella enterica subsp. enterica serovar Typhimurium str. LT2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3254953..3265933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STM3093 endA 3255411..3256118 (+) 708 NP_462009.1 DNA-specific endonuclease I -
  STM3094 yggJ 3256147..3256926 (+) 780 NP_462010.3 putative cytoplasmic protein -
  STM3095 gshB 3256946..3257893 (+) 948 NP_462011.1 glutathione synthetase -
  STM3096 yqgE 3258109..3258672 (+) 564 NP_462012.1 putative transcriptional regulator -
  STM3097 yqgF 3258672..3259088 (+) 417 NP_462013.1 putative endonuclease involved in recombination -
  STM3098 - 3259135..3259821 (-) 687 NP_462014.1 putative transcriptional regulator -
  STM3099 pilT 3259953..3260933 (-) 981 NP_462015.1 putative protein transport Machinery gene
  STM3100 yggS 3260951..3261655 (+) 705 NP_462016.1 putative enzyme with a TIM-barrel fold -
  STM3101 yggT 3261674..3262240 (+) 567 NP_462017.1 putative integral membran resistance protein -
  STM3102 yggU 3262237..3262527 (+) 291 NP_462018.1 putative cytoplasmic protein -
  STM3103 yggV 3262535..3263128 (+) 594 NP_462019.1 putative xanthosine triphosphate pyrophosphatase -
  STM3104 yggW 3263121..3264257 (+) 1137 NP_462020.1 putative oxidase -
  STM3105 yggM 3264348..3265355 (-) 1008 NP_462021.1 putative periplasmic protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36150.38 Da        Isoelectric Point: 8.0973

>NTDB_id=21714 STM3099 NP_462015.1 3259953..3260933(-) (pilT) [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=21714 STM3099 NP_462015.1 3259953..3260933(-) (pilT) [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTGAAGATCCTGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8ZM48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.556

100

0.371
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362

Multiple sequence alignment