Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PspS49_RS27725 Genome accession   NZ_CP019432
Coordinates   6247114..6248148 (-) Length   344 a.a.
NCBI ID   WP_095049465.1    Uniprot ID   -
Organism   Pseudomonas sp. S49     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6242114..6253148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS49_RS27695 (PspS49_28025) ruvX 6242273..6242710 (+) 438 WP_008077844.1 Holliday junction resolvase RuvX -
  PspS49_RS27700 (PspS49_28030) pyrR 6242791..6243297 (+) 507 WP_034153653.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PspS49_RS27705 (PspS49_28035) - 6243325..6244329 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  PspS49_RS27710 (PspS49_28040) - 6244326..6245597 (+) 1272 WP_110718025.1 dihydroorotase -
  PspS49_RS27715 (PspS49_28045) - 6245691..6246122 (-) 432 WP_110718026.1 TM2 domain-containing protein -
  PspS49_RS27720 (PspS49_28050) - 6246371..6246988 (+) 618 WP_127930107.1 C40 family peptidase -
  PspS49_RS27725 (PspS49_28055) pilT 6247114..6248148 (-) 1035 WP_095049465.1 type IV pilus twitching motility protein PilT Machinery gene
  PspS49_RS27730 (PspS49_28060) - 6248205..6248891 (+) 687 WP_160042201.1 YggS family pyridoxal phosphate-dependent enzyme -
  PspS49_RS27735 (PspS49_28065) proC 6248915..6249733 (+) 819 WP_150693739.1 pyrroline-5-carboxylate reductase -
  PspS49_RS27740 (PspS49_28070) - 6249744..6250334 (+) 591 WP_150776071.1 YggT family protein -
  PspS49_RS27745 (PspS49_28075) - 6250331..6250618 (+) 288 WP_127930111.1 DUF167 domain-containing protein -
  PspS49_RS27750 (PspS49_28080) - 6250798..6251937 (+) 1140 WP_150749753.1 homoserine O-acetyltransferase -
  PspS49_RS27755 (PspS49_28085) metW 6251945..6252565 (+) 621 WP_127930113.1 methionine biosynthesis protein MetW -
  PspS49_RS27760 (PspS49_28090) - 6252592..6253026 (+) 435 WP_160042203.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38085.76 Da        Isoelectric Point: 7.0157

>NTDB_id=215768 PspS49_RS27725 WP_095049465.1 6247114..6248148(-) (pilT) [Pseudomonas sp. S49]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLLTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=215768 PspS49_RS27725 WP_095049465.1 6247114..6248148(-) (pilT) [Pseudomonas sp. S49]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGAGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAACTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTTGAAGTGCCGGGTGTCGCGCGG
TTCCGGGTTAACGCCTTCAACCAGAACCGTGGCGCTGGCGCCGTATTCCGTACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTCCGTAAGATCACCGACGCCCCGCGTGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGATTACCTCAACACTCACCGTCATCACCATATCCTGACTATTGAAGAC
CCGATCGAGTTTGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGTGATACCCGCAGTTTCGCCAC
CGCTCTGCGCTCGGCGTTGCGCGAAGACCCGGACGTGATTCTGGTCGGCGAGATGCGTGACCTGGAAACCATTCGCCTGG
CACTGACCGCAGCCGAAACCGGGCATCTGGTGTTCGGCACGCTGCACACCACCTCAGCGGCGAAAACCATCGACCGCGTG
GTCGACGTATTCCCCGGTGACGAGAAGTCGATGGTGCGCTCGATGCTGTCGGAGTCGTTGCTGGCGGTGGTCTCGCAGAC
GTTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCACGAGATCATGCTGGGCACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATTCAGACCGGTGGGTCGTTGGGCATGCAGACACTGGATATGTGCCTG
AAGGATCTGCTGACCAAGGGCTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.42

100

0.395

Multiple sequence alignment