Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PspS49_RS25020 Genome accession   NZ_CP019432
Coordinates   5678605..5679036 (-) Length   143 a.a.
NCBI ID   WP_034153877.1    Uniprot ID   A0A7Z2REX4
Organism   Pseudomonas sp. S49     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5673605..5684036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS49_RS25005 (PspS49_25325) - 5674432..5677383 (+) 2952 WP_160041864.1 DUF748 domain-containing protein -
  PspS49_RS25010 (PspS49_25330) - 5677383..5677679 (+) 297 WP_160041866.1 DUF2845 domain-containing protein -
  PspS49_RS25015 (PspS49_25335) - 5677950..5678306 (-) 357 WP_047600285.1 BON domain-containing protein -
  PspS49_RS25020 (PspS49_25340) pilA 5678605..5679036 (-) 432 WP_034153877.1 pilin Machinery gene
  PspS49_RS25025 (PspS49_25345) pilB 5679263..5680963 (+) 1701 WP_160041868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PspS49_RS25030 (PspS49_25350) pilC 5680966..5682183 (+) 1218 WP_127929816.1 type II secretion system F family protein Machinery gene
  PspS49_RS25035 (PspS49_25355) pilD 5682185..5683057 (+) 873 WP_150774963.1 A24 family peptidase Machinery gene
  PspS49_RS25040 (PspS49_25360) coaE 5683054..5683677 (+) 624 WP_160041870.1 dephospho-CoA kinase -
  PspS49_RS25045 (PspS49_25365) yacG 5683674..5683874 (+) 201 WP_007909902.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14323.47 Da        Isoelectric Point: 8.4936

>NTDB_id=215758 PspS49_RS25020 WP_034153877.1 5678605..5679036(-) (pilA) [Pseudomonas sp. S49]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLTLVAGSATAATSNCT
LAASGTASTGAGTITCTLLNAPGPVLSKTITLTRSSGTGAGVWTCGTTVAPDYSPKGCTASGT

Nucleotide


Download         Length: 432 bp        

>NTDB_id=215758 PspS49_RS25020 WP_034153877.1 5678605..5679036(-) (pilA) [Pseudomonas sp. S49]
ATGAAAAAACAACAAGGTTTTACTCTGATCGAACTGCTGATCGTGGTGGCCATCATCGGGATTCTGGCGACCATCGCCTT
GCCGCAGTATTCCAAGTATCAGGCGCGGTCGAAGGTAACGGCGGGGCTGGCGGAGATCAGTGCGCTGAAGGTGCCGTTTG
AAGACACCATCAATCAAGGCACCGATCCAACGCTGACATTGGTTGCGGGTAGTGCGACGGCGGCGACATCCAACTGCACA
CTCGCCGCATCCGGTACTGCATCCACCGGTGCCGGAACCATCACTTGCACACTGCTCAATGCCCCGGGTCCGGTGCTGAG
CAAGACCATCACCCTGACTCGTTCTTCAGGTACCGGCGCGGGTGTATGGACCTGCGGTACCACCGTTGCTCCGGATTATT
CCCCTAAAGGCTGCACCGCCAGCGGTACATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2REX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae C6706

45.395

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.395

100

0.483
  pilA Vibrio cholerae strain A1552

45.395

100

0.483
  pilA Acinetobacter baumannii strain A118

47.887

99.301

0.476
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.928

96.503

0.434
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.434
  comP Acinetobacter baylyi ADP1

39.61

100

0.427
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.478

96.503

0.42
  pilA Vibrio parahaemolyticus RIMD 2210633

40.278

100

0.406
  pilA2 Legionella pneumophila str. Paris

40.714

97.902

0.399
  pilA2 Legionella pneumophila strain ERS1305867

40.714

97.902

0.399
  pilA Ralstonia pseudosolanacearum GMI1000

34.783

100

0.392

Multiple sequence alignment