Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PspS49_RS25025 Genome accession   NZ_CP019432
Coordinates   5679263..5680963 (+) Length   566 a.a.
NCBI ID   WP_160041868.1    Uniprot ID   A0A7Z2RFR6
Organism   Pseudomonas sp. S49     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5674263..5685963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS49_RS25005 (PspS49_25325) - 5674432..5677383 (+) 2952 WP_160041864.1 DUF748 domain-containing protein -
  PspS49_RS25010 (PspS49_25330) - 5677383..5677679 (+) 297 WP_160041866.1 DUF2845 domain-containing protein -
  PspS49_RS25015 (PspS49_25335) - 5677950..5678306 (-) 357 WP_047600285.1 BON domain-containing protein -
  PspS49_RS25020 (PspS49_25340) pilA 5678605..5679036 (-) 432 WP_034153877.1 pilin Machinery gene
  PspS49_RS25025 (PspS49_25345) pilB 5679263..5680963 (+) 1701 WP_160041868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PspS49_RS25030 (PspS49_25350) pilC 5680966..5682183 (+) 1218 WP_127929816.1 type II secretion system F family protein Machinery gene
  PspS49_RS25035 (PspS49_25355) pilD 5682185..5683057 (+) 873 WP_150774963.1 A24 family peptidase Machinery gene
  PspS49_RS25040 (PspS49_25360) coaE 5683054..5683677 (+) 624 WP_160041870.1 dephospho-CoA kinase -
  PspS49_RS25045 (PspS49_25365) yacG 5683674..5683874 (+) 201 WP_007909902.1 DNA gyrase inhibitor YacG -
  PspS49_RS25050 (PspS49_25370) - 5683881..5684096 (-) 216 WP_007909901.1 hypothetical protein -
  PspS49_RS25055 (PspS49_25375) - 5684162..5684851 (-) 690 WP_150774964.1 energy-coupling factor ABC transporter permease -
  PspS49_RS25060 (PspS49_25380) - 5685044..5685670 (+) 627 WP_127929820.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62482.44 Da        Isoelectric Point: 5.9461

>NTDB_id=215759 PspS49_RS25025 WP_160041868.1 5679263..5680963(+) (pilB) [Pseudomonas sp. S49]
MNDIALSGLAKQLVQAELLTEKSAQQASQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTNASGLEDMADVDLDG
LDVESIDDSKQDAIAGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSRSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKELYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=215759 PspS49_RS25025 WP_160041868.1 5679263..5680963(+) (pilB) [Pseudomonas sp. S49]
ATGAACGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
CTCGCAACAGGCCCAGCGTAATCGCTTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTCACAGGTCG
CCGAAATCGCCTCCGAGCATTTCGGCATGGCCTTGCTTGATCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCCTGTGGCGACGTGGCAACAAGCTGTTCGTAGGCATTTCCGA
CCCGAGCAATCATCAAGCGATCAACGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCAGCGATGCCATCGAAAAATTCTTCGACACCAACGCCAGCGGTCTGGAGGACATGGCCGATGTCGATCTCGATGGC
CTCGATGTCGAATCCATCGACGACAGCAAACAGGACGCCATCGCAGGCCTCGACGCCGACGATGCACCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAAAGCGGCTCATCCGACTTGCACTTCGAACCTTACGAGAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTGGCCAAGCCACCAATTCAACTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGATATTTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAG
GTCGATCGATTTTCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CACAGATCGGCATCGACGCGCTCGGTTACGAACCCGCGCAGAAAGAACTGTACATGGCCGCGCTCAAGCAGCCCCAAGGC
ATGATTCTGGTCACTGGTCCCACCGGCTCGGGGAAAACCGTGTCGCTGTACACCGGCCTGAATATCCTCAACACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAAG
GGCTCGACTTTGCCCAGGCACTGCGCTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAAGCCGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATTCCCGGCTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCACGCAAGCTCTGTAGCCATTGCAAGAAAGCCATCGAGATCCCCCGCGAAACCTTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCAGAGCTGCAACGTTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACCTGCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2RFR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.156

99.647

0.58
  pilB Acinetobacter baylyi ADP1

58.082

99.47

0.578
  pilB Legionella pneumophila strain ERS1305867

56.89

100

0.569
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.293

0.496
  pilB Vibrio campbellii strain DS40M4

48.438

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.463

0.366
  pilF Thermus thermophilus HB27

36.918

98.587

0.364

Multiple sequence alignment