Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PspS49_RS25030 Genome accession   NZ_CP019432
Coordinates   5680966..5682183 (+) Length   405 a.a.
NCBI ID   WP_127929816.1    Uniprot ID   -
Organism   Pseudomonas sp. S49     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5675966..5687183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS49_RS25010 (PspS49_25330) - 5677383..5677679 (+) 297 WP_160041866.1 DUF2845 domain-containing protein -
  PspS49_RS25015 (PspS49_25335) - 5677950..5678306 (-) 357 WP_047600285.1 BON domain-containing protein -
  PspS49_RS25020 (PspS49_25340) pilA 5678605..5679036 (-) 432 WP_034153877.1 pilin Machinery gene
  PspS49_RS25025 (PspS49_25345) pilB 5679263..5680963 (+) 1701 WP_160041868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PspS49_RS25030 (PspS49_25350) pilC 5680966..5682183 (+) 1218 WP_127929816.1 type II secretion system F family protein Machinery gene
  PspS49_RS25035 (PspS49_25355) pilD 5682185..5683057 (+) 873 WP_150774963.1 A24 family peptidase Machinery gene
  PspS49_RS25040 (PspS49_25360) coaE 5683054..5683677 (+) 624 WP_160041870.1 dephospho-CoA kinase -
  PspS49_RS25045 (PspS49_25365) yacG 5683674..5683874 (+) 201 WP_007909902.1 DNA gyrase inhibitor YacG -
  PspS49_RS25050 (PspS49_25370) - 5683881..5684096 (-) 216 WP_007909901.1 hypothetical protein -
  PspS49_RS25055 (PspS49_25375) - 5684162..5684851 (-) 690 WP_150774964.1 energy-coupling factor ABC transporter permease -
  PspS49_RS25060 (PspS49_25380) - 5685044..5685670 (+) 627 WP_127929820.1 DUF1780 domain-containing protein -
  PspS49_RS25065 (PspS49_25385) - 5685667..5686194 (+) 528 WP_127929821.1 MOSC domain-containing protein -
  PspS49_RS25070 (PspS49_25390) - 5686246..5686419 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44067.78 Da        Isoelectric Point: 10.1093

>NTDB_id=215760 PspS49_RS25030 WP_127929816.1 5680966..5682183(+) (pilC) [Pseudomonas sp. S49]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGISPGKVRKKSASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ERLKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILGALVAAIFGTR
HALKTSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDEMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=215760 PspS49_RS25030 WP_127929816.1 5680966..5682183(+) (pilC) [Pseudomonas sp. S49]
ATGGCGGTCAAGGCAGCGAAAATCAGTATTTATGCCTGGGAAGGTACGGACAGGAAAGGCAGCAAAGTCACCGGTGAACT
CAGTGGACAGAATCCGGCACTGATCAAGGCCCAACTGCGTAAACAGGGGATCAGTCCCGGCAAGGTACGCAAAAAATCCG
CGTCGTTGTTGAGTTTTGGCAAACGCATCAAGGCCCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATTGGCGAAGGTTTTGAAAACCCGGCCATGCGCAAGCTGGTGGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCCACAGCCCTGCGCAAGAAGCCGCAGTATTTCGACGAGTTGTATT
GCAACCTGGTCGATGCCGGCGAGCAGTCCGGCGCCCTCGACACGCTGCTGGAACGGGTCGCAACCTATAAGGAAAAAAGC
GAACGGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTCGTACTGGTCGCAGCGGTCGTGACCGGAAT
TCTGCTGGTTAAAGTGGTGCCGCAATTCCAGTCGGTATTCTCGGGGTTCGGTGCAGAGTTACCGGCCTTCACCCTGATGG
TGATCAGCCTTTCGGAATTCATGCAGCAATGGTGGTGGGCGATTCTCGGCGCGCTGGTCGCGGCGATTTTCGGCACCCGT
CACGCACTGAAAACCTCCCAGGCGCTACGTGATCGCAAAGACACCTGGCTGTTGAAACTGCCCTTGGTCGGCACGCTGAT
GTACAAGTCAGCGGTAGCCCGTTTCGCTCGAACCCTGTCGACCACGTTTGCTGCCGGTGTGCCATTGGTGGAAGCGCTCG
ACTCGGTGGCCGGCGCTACCGGCAATGTGGTGTTCAAGCGTGCCGTGCTGCGCATTCGCCAAGACGTCTCCACCGGCATG
CAGCTGAATTTTTCGATGCGCAGCACCGGAGTCTTTCCCAACATGGCCGTGCAGATGACCGCGATTGGCGAAGAGTCGGG
GGCGCTAGATGAAATGCTCGACAAGGTTGCAGGCTTTTACGAGGAAGAAGTGGATAACATGGTCGACAATCTCACCAGCC
TCATGGAGCCGTTCATCATGGTCGTGCTGGGGGTGATCGTCGGCGGGCTGGTGGTAGCCATGTACCTGCCGATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

38.903

99.012

0.385

Multiple sequence alignment