Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BVG84_RS18085 Genome accession   NZ_CP018930
Coordinates   3773688..3774710 (-) Length   340 a.a.
NCBI ID   WP_043141339.1    Uniprot ID   -
Organism   Serratia marcescens strain UMH12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3768688..3779710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG84_RS18055 (BVG84_18100) - 3769487..3769999 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  BVG84_RS18060 (BVG84_18105) endA 3770101..3770796 (+) 696 WP_043141331.1 deoxyribonuclease I -
  BVG84_RS18065 (BVG84_18110) rsmE 3770866..3771597 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BVG84_RS18070 (BVG84_18115) gshB 3771608..3772558 (+) 951 WP_043141334.1 glutathione synthase -
  BVG84_RS18075 (BVG84_18120) - 3772706..3773269 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  BVG84_RS18080 (BVG84_18125) ruvX 3773269..3773691 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  BVG84_RS18085 (BVG84_18130) pilT 3773688..3774710 (-) 1023 WP_043141339.1 type IV pilus twitching motility protein PilT Machinery gene
  BVG84_RS18090 (BVG84_18135) - 3774731..3775438 (+) 708 WP_043141342.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVG84_RS18095 (BVG84_18140) proC 3775458..3776279 (+) 822 WP_025304249.1 pyrroline-5-carboxylate reductase -
  BVG84_RS18100 (BVG84_18145) - 3776311..3776865 (+) 555 WP_025304250.1 YggT family protein -
  BVG84_RS18105 (BVG84_18150) yggU 3776862..3777152 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  BVG84_RS18110 (BVG84_18155) - 3777170..3777763 (+) 594 WP_043141349.1 XTP/dITP diphosphatase -
  BVG84_RS18115 (BVG84_18160) hemW 3777756..3778898 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  BVG84_RS18120 (BVG84_18165) - 3778936..3779184 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  BVG84_RS18125 (BVG84_18170) - 3779168..3779410 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36559.78 Da        Isoelectric Point: 7.2889

>NTDB_id=212063 BVG84_RS18085 WP_043141339.1 3773688..3774710(-) (pilT) [Serratia marcescens strain UMH12]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANIFQQSAGMSLALRRIARQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=212063 BVG84_RS18085 WP_043141339.1 3773688..3774710(-) (pilT) [Serratia marcescens strain UMH12]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACATTTTTCAACAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGTATTGCCAGGCAATCGCCTTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCAGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCTGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Pseudomonas aeruginosa PAK

49.254

98.529

0.485
  pilT Neisseria gonorrhoeae MS11

49.254

98.529

0.485
  pilT Neisseria meningitidis 8013

49.849

97.353

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.353

0.476
  pilT Legionella pneumophila strain ERS1305867

48.943

97.353

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment