Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BVG93_RS18195 Genome accession   NZ_CP018926
Coordinates   3776498..3777520 (-) Length   340 a.a.
NCBI ID   WP_089192249.1    Uniprot ID   -
Organism   Serratia marcescens strain UMH6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3771498..3782520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG93_RS18165 (BVG93_18180) - 3772299..3772811 (+) 513 WP_089192248.1 SprT family zinc-dependent metalloprotease -
  BVG93_RS18170 (BVG93_18185) endA 3772913..3773608 (+) 696 WP_025304243.1 deoxyribonuclease I -
  BVG93_RS18175 (BVG93_18190) rsmE 3773678..3774409 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BVG93_RS18180 (BVG93_18195) gshB 3774420..3775370 (+) 951 WP_016930084.1 glutathione synthase -
  BVG93_RS18185 (BVG93_18200) - 3775516..3776079 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  BVG93_RS18190 (BVG93_18205) ruvX 3776079..3776501 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  BVG93_RS18195 (BVG93_18210) pilT 3776498..3777520 (-) 1023 WP_089192249.1 type IV pilus twitching motility protein PilT Machinery gene
  BVG93_RS18200 (BVG93_18215) - 3777541..3778248 (+) 708 WP_042785369.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVG93_RS18205 (BVG93_18220) proC 3778268..3779089 (+) 822 WP_033644672.1 pyrroline-5-carboxylate reductase -
  BVG93_RS18210 (BVG93_18225) - 3779121..3779675 (+) 555 WP_042785370.1 YggT family protein -
  BVG93_RS18215 (BVG93_18230) yggU 3779672..3779962 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  BVG93_RS18220 (BVG93_18235) - 3779980..3780573 (+) 594 WP_048796773.1 XTP/dITP diphosphatase -
  BVG93_RS18225 (BVG93_18240) hemW 3780566..3781708 (+) 1143 WP_089192250.1 radical SAM family heme chaperone HemW -
  BVG93_RS18230 (BVG93_18245) - 3781745..3781993 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  BVG93_RS18235 (BVG93_18250) - 3781977..3782219 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36491.78 Da        Isoelectric Point: 7.5736

>NTDB_id=211969 BVG93_RS18195 WP_089192249.1 3776498..3777520(-) (pilT) [Serratia marcescens strain UMH6]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILITGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLTTLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=211969 BVG93_RS18195 WP_089192249.1 3776498..3777520(-) (pilT) [Serratia marcescens strain UMH6]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAACCGCTGGCGGCGGCGCAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAACAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGATCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCATCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGACGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAATCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

97.059

0.524
  pilT Vibrio cholerae strain A1552

53.939

97.059

0.524
  pilT Neisseria meningitidis 8013

49.412

100

0.494
  pilT Pseudomonas aeruginosa PAK

48.555

100

0.494
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Neisseria gonorrhoeae MS11

48.817

99.412

0.485
  pilT Acinetobacter nosocomialis M2

50

97.059

0.485
  pilT Acinetobacter baumannii D1279779

50

97.059

0.485
  pilT Acinetobacter baumannii strain A118

50

97.059

0.485
  pilT Pseudomonas stutzeri DSM 10701

47.788

99.706

0.476
  pilT Legionella pneumophila strain Lp02

47.647

100

0.476
  pilT Legionella pneumophila strain ERS1305867

47.647

100

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.417

100

0.456
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.053

99.706

0.379
  pilU Vibrio cholerae strain A1552

38.957

95.882

0.374

Multiple sequence alignment