Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BVG88_RS18075 Genome accession   NZ_CP018924
Coordinates   3784622..3785644 (-) Length   340 a.a.
NCBI ID   WP_089195156.1    Uniprot ID   -
Organism   Serratia marcescens strain UMH2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3779622..3790644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG88_RS18045 (BVG88_18070) - 3780421..3780933 (+) 513 WP_033651326.1 SprT family zinc-dependent metalloprotease -
  BVG88_RS18050 (BVG88_18075) endA 3781035..3781730 (+) 696 WP_033644681.1 deoxyribonuclease I -
  BVG88_RS18055 (BVG88_18080) rsmE 3781800..3782531 (+) 732 WP_089195155.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BVG88_RS18060 (BVG88_18085) gshB 3782542..3783492 (+) 951 WP_033651325.1 glutathione synthase -
  BVG88_RS18065 (BVG88_18090) - 3783640..3784203 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  BVG88_RS18070 (BVG88_18095) ruvX 3784203..3784625 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  BVG88_RS18075 (BVG88_18100) pilT 3784622..3785644 (-) 1023 WP_089195156.1 type IV pilus twitching motility protein PilT Machinery gene
  BVG88_RS18080 (BVG88_18105) - 3785665..3786372 (+) 708 WP_089195157.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVG88_RS18085 (BVG88_18110) proC 3786392..3787213 (+) 822 WP_089195158.1 pyrroline-5-carboxylate reductase -
  BVG88_RS18090 (BVG88_18115) - 3787245..3787799 (+) 555 WP_089195159.1 YggT family protein -
  BVG88_RS18095 (BVG88_18120) yggU 3787796..3788086 (+) 291 WP_074055088.1 DUF167 family protein YggU -
  BVG88_RS18100 (BVG88_18125) - 3788104..3788697 (+) 594 WP_042785371.1 XTP/dITP diphosphatase -
  BVG88_RS18105 (BVG88_18130) hemW 3788690..3789832 (+) 1143 WP_089192250.1 radical SAM family heme chaperone HemW -
  BVG88_RS18110 (BVG88_18135) - 3789870..3790304 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36391.66 Da        Isoelectric Point: 7.2888

>NTDB_id=211921 BVG88_RS18075 WP_089195156.1 3784622..3785644(-) (pilT) [Serratia marcescens strain UMH2]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGVQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=211921 BVG88_RS18075 WP_089195156.1 3784622..3785644(-) (pilT) [Serratia marcescens strain UMH2]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTACGTGCGCGCCCAGTTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGTGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment