Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BVG94_RS17880 Genome accession   NZ_CP018919
Coordinates   3736380..3737402 (-) Length   340 a.a.
NCBI ID   WP_089197423.1    Uniprot ID   -
Organism   Serratia marcescens strain UMH7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3731380..3742402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG94_RS17850 (BVG94_17885) - 3732181..3732693 (+) 513 WP_033644682.1 SprT family zinc-dependent metalloprotease -
  BVG94_RS17855 (BVG94_17890) endA 3732795..3733490 (+) 696 WP_033644681.1 deoxyribonuclease I -
  BVG94_RS17860 (BVG94_17895) rsmE 3733560..3734291 (+) 732 WP_033644679.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BVG94_RS17865 (BVG94_17900) gshB 3734302..3735252 (+) 951 WP_033644677.1 glutathione synthase -
  BVG94_RS17870 (BVG94_17905) - 3735398..3735961 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  BVG94_RS17875 (BVG94_17910) ruvX 3735961..3736383 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  BVG94_RS17880 (BVG94_17915) pilT 3736380..3737402 (-) 1023 WP_089197423.1 type IV pilus twitching motility protein PilT Machinery gene
  BVG94_RS17885 (BVG94_17920) - 3737423..3738130 (+) 708 WP_089197424.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVG94_RS17890 (BVG94_17925) proC 3738150..3738971 (+) 822 WP_089197425.1 pyrroline-5-carboxylate reductase -
  BVG94_RS17895 (BVG94_17930) - 3739003..3739557 (+) 555 WP_033644671.1 YggT family protein -
  BVG94_RS17900 (BVG94_17935) yggU 3739554..3739844 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  BVG94_RS17905 (BVG94_17940) - 3739862..3740455 (+) 594 WP_089197426.1 XTP/dITP diphosphatase -
  BVG94_RS17910 (BVG94_17945) hemW 3740448..3741590 (+) 1143 WP_033644669.1 radical SAM family heme chaperone HemW -
  BVG94_RS17915 (BVG94_17950) - 3741628..3742062 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36424.64 Da        Isoelectric Point: 7.2888

>NTDB_id=211875 BVG94_RS17880 WP_089197423.1 3736380..3737402(-) (pilT) [Serratia marcescens strain UMH7]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTATETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=211875 BVG94_RS17880 WP_089197423.1 3736380..3737402(-) (pilT) [Serratia marcescens strain UMH7]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCACAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGACGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTACGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCAAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

53.939

97.059

0.524
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

97.059

0.524
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii D1279779

50

97.059

0.485
  pilT Acinetobacter baumannii strain A118

50

97.059

0.485
  pilT Acinetobacter nosocomialis M2

50

97.059

0.485
  pilT Neisseria meningitidis 8013

49.547

97.353

0.482
  pilT Neisseria gonorrhoeae MS11

49.245

97.353

0.479
  pilT Legionella pneumophila strain ERS1305867

48.64

97.353

0.474
  pilT Legionella pneumophila strain Lp02

48.64

97.353

0.474
  pilT Pseudomonas stutzeri DSM 10701

47.774

99.118

0.474
  pilT Pseudomonas aeruginosa PAK

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

37.963

95.294

0.362

Multiple sequence alignment