Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BVG97_RS18440 Genome accession   NZ_CP018917
Coordinates   3821767..3822789 (-) Length   340 a.a.
NCBI ID   WP_084827146.1    Uniprot ID   -
Organism   Serratia marcescens strain UMH5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3816767..3827789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG97_RS18410 (BVG97_18415) - 3817568..3818080 (+) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -
  BVG97_RS18415 (BVG97_18420) endA 3818182..3818877 (+) 696 WP_038879275.1 deoxyribonuclease I -
  BVG97_RS18420 (BVG97_18425) rsmE 3818947..3819678 (+) 732 WP_060430995.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BVG97_RS18425 (BVG97_18430) gshB 3819689..3820639 (+) 951 WP_047729854.1 glutathione synthase -
  BVG97_RS18430 (BVG97_18435) - 3820785..3821348 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  BVG97_RS18435 (BVG97_18440) ruvX 3821348..3821770 (+) 423 WP_084827147.1 Holliday junction resolvase RuvX -
  BVG97_RS18440 (BVG97_18445) pilT 3821767..3822789 (-) 1023 WP_084827146.1 type IV pilus twitching motility protein PilT Machinery gene
  BVG97_RS18445 (BVG97_18450) - 3822810..3823517 (+) 708 WP_084827145.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVG97_RS18450 (BVG97_18455) proC 3823537..3824358 (+) 822 WP_089186457.1 pyrroline-5-carboxylate reductase -
  BVG97_RS18455 (BVG97_18460) - 3824390..3824944 (+) 555 WP_084827144.1 YggT family protein -
  BVG97_RS18460 (BVG97_18465) yggU 3824941..3825231 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  BVG97_RS18465 (BVG97_18470) - 3825249..3825842 (+) 594 WP_084827143.1 XTP/dITP diphosphatase -
  BVG97_RS18470 (BVG97_18475) hemW 3825835..3826977 (+) 1143 WP_084827142.1 radical SAM family heme chaperone HemW -
  BVG97_RS18475 (BVG97_18480) - 3827015..3827449 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36487.85 Da        Isoelectric Point: 7.2889

>NTDB_id=211851 BVG97_RS18440 WP_084827146.1 3821767..3822789(-) (pilT) [Serratia marcescens strain UMH5]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQLDLALHRPGGE
RLRANVFQQRAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDTHSFDAALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDASVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=211851 BVG97_RS18440 WP_084827146.1 3821767..3822789(-) (pilT) [Serratia marcescens strain UMH5]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGCTCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAACGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCACCTTCGCT
TGCCGAGCTGGCGGCCCCGGCCATCGTCCCGGCACTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTAACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGACGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACACTCACAGCTTCGA
TGCGGCGTTGCGCGCTGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTCGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCAGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.077

95.588

0.526
  pilT Vibrio cholerae strain A1552

55.077

95.588

0.526
  pilT Acinetobacter baylyi ADP1

52.632

95

0.5
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria meningitidis 8013

51.703

95

0.491
  pilT Acinetobacter baumannii D1279779

51.863

94.706

0.491
  pilT Acinetobacter baumannii strain A118

51.863

94.706

0.491
  pilT Neisseria gonorrhoeae MS11

51.393

95

0.488
  pilT Pseudomonas aeruginosa PAK

50.932

94.706

0.482
  pilT Legionella pneumophila strain ERS1305867

49.547

97.353

0.482
  pilT Legionella pneumophila strain Lp02

49.547

97.353

0.482
  pilT Pseudomonas stutzeri DSM 10701

50.311

94.706

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.906

100

0.462
  pilU Vibrio cholerae strain A1552

40.741

95.294

0.388
  pilU Acinetobacter baylyi ADP1

40.557

95

0.385
  pilU Pseudomonas stutzeri DSM 10701

39.506

95.294

0.376

Multiple sequence alignment