Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BVG95_RS17245 Genome accession   NZ_CP018915
Coordinates   3637825..3638847 (-) Length   340 a.a.
NCBI ID   WP_089196265.1    Uniprot ID   -
Organism   Serratia marcescens strain UMH1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3632825..3643847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG95_RS17215 (BVG95_17235) - 3633624..3634136 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  BVG95_RS17220 (BVG95_17240) endA 3634238..3634933 (+) 696 WP_089196263.1 deoxyribonuclease I -
  BVG95_RS17225 (BVG95_17245) rsmE 3635003..3635734 (+) 732 WP_089196264.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BVG95_RS17230 (BVG95_17250) gshB 3635745..3636695 (+) 951 WP_043141334.1 glutathione synthase -
  BVG95_RS17235 (BVG95_17255) - 3636843..3637406 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  BVG95_RS17240 (BVG95_17260) ruvX 3637406..3637828 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  BVG95_RS17245 (BVG95_17265) pilT 3637825..3638847 (-) 1023 WP_089196265.1 type IV pilus twitching motility protein PilT Machinery gene
  BVG95_RS17250 (BVG95_17270) - 3638868..3639575 (+) 708 WP_060434540.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVG95_RS17255 (BVG95_17275) proC 3639595..3640416 (+) 822 WP_025304249.1 pyrroline-5-carboxylate reductase -
  BVG95_RS17260 (BVG95_17280) - 3640448..3641002 (+) 555 WP_025304250.1 YggT family protein -
  BVG95_RS17265 (BVG95_17285) yggU 3640999..3641289 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  BVG95_RS17270 (BVG95_17290) - 3641307..3641900 (+) 594 WP_043141349.1 XTP/dITP diphosphatase -
  BVG95_RS17275 (BVG95_17295) hemW 3641893..3643035 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  BVG95_RS17280 (BVG95_17300) - 3643073..3643321 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  BVG95_RS17285 (BVG95_17305) - 3643305..3643547 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36473.69 Da        Isoelectric Point: 7.0670

>NTDB_id=211828 BVG95_RS17245 WP_089196265.1 3637825..3638847(-) (pilT) [Serratia marcescens strain UMH1]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGMSLALRRIAGQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=211828 BVG95_RS17245 WP_089196265.1 3637825..3638847(-) (pilT) [Serratia marcescens strain UMH1]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCACAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGTGGAGAG
CGGCTGCGTGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGTCGCCCTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTCGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.851

98.529

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.665

99.118

0.482
  pilT Legionella pneumophila strain ERS1305867

49.547

97.353

0.482
  pilT Legionella pneumophila strain Lp02

49.547

97.353

0.482
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

40.184

95.882

0.385
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment