Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BUQ72_RS21095 Genome accession   NZ_CP018845
Coordinates   4743845..4744888 (-) Length   347 a.a.
NCBI ID   WP_079218330.1    Uniprot ID   A0A246WMF8
Organism   Herbaspirillum robiniae strain AA6     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4738845..4749888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUQ72_RS21070 - 4738947..4741244 (+) 2298 WP_420809805.1 bifunctional diguanylate cyclase/phosphodiesterase -
  BUQ72_RS21075 - 4741241..4742128 (+) 888 WP_079218327.1 phosphate/phosphite/phosphonate ABC transporter substrate-binding protein -
  BUQ72_RS21080 - 4742209..4742853 (+) 645 WP_079218328.1 glutathione S-transferase -
  BUQ72_RS24830 - 4742876..4743256 (+) 381 WP_237772573.1 gamma-glutamylcyclotransferase family protein -
  BUQ72_RS21090 - 4743284..4743769 (-) 486 WP_079218329.1 glutathione peroxidase -
  BUQ72_RS21095 pilT 4743845..4744888 (-) 1044 WP_079218330.1 type IV pilus twitching motility protein PilT Machinery gene
  BUQ72_RS21100 - 4744961..4745662 (+) 702 WP_079218331.1 YggS family pyridoxal phosphate-dependent enzyme -
  BUQ72_RS21105 proC 4745770..4746582 (+) 813 WP_079218332.1 pyrroline-5-carboxylate reductase -
  BUQ72_RS21110 - 4746582..4747331 (+) 750 WP_079218333.1 peptidoglycan DD-metalloendopeptidase family protein -
  BUQ72_RS21115 - 4747346..4747573 (+) 228 WP_079218334.1 hypothetical protein -
  BUQ72_RS21125 - 4747955..4748626 (-) 672 WP_079218336.1 thiopurine S-methyltransferase -
  BUQ72_RS21130 ubiA 4748667..4749521 (-) 855 WP_079218337.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37386.91 Da        Isoelectric Point: 7.1983

>NTDB_id=210967 BUQ72_RS21095 WP_079218330.1 4743845..4744888(-) (pilT) [Herbaspirillum robiniae strain AA6]
MDIAGLLAFAVRNQASDLHLSAGLPPMLRVHGDIRRINLTALSDEQLQSMLQSVMTEAQRAAYAERMECDFSFHRPGLGR
FRVNAYRQQRGAAAAIRHIPQQIPALTAIGAPAVCAELALRPRGLVLVTGATGSGKSTTLAAMVGHINEHRAAHIITVED
PIEFIHEPRRSLVSQRELGAHTRSFADALRGALREDPDVLLVGELRDAETIRLALTAAETGHLVFGTLHTASAAKTVDRI
VDVFAPGEKDGARAMLAESLQAVISQTLLPAPDGHSRVAAHEVMLCTPAIRNLIREGKTAQLYSAIQTATGLGMQTLEGC
LGELVHAERITLETARAHARSPDFFPM

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=210967 BUQ72_RS21095 WP_079218330.1 4743845..4744888(-) (pilT) [Herbaspirillum robiniae strain AA6]
ATGGACATTGCCGGACTACTGGCATTTGCCGTGCGAAACCAGGCCTCGGACCTGCACTTGTCAGCCGGACTTCCACCCAT
GCTGCGCGTGCATGGCGACATCCGGCGCATCAACCTGACCGCGCTTTCCGACGAGCAACTGCAGAGCATGCTGCAGTCGG
TGATGACCGAGGCGCAGCGCGCCGCCTATGCCGAGCGCATGGAGTGCGACTTCTCCTTCCACCGTCCGGGCCTGGGGCGC
TTTCGCGTCAACGCCTATCGCCAGCAACGCGGCGCGGCCGCGGCCATACGCCACATCCCGCAGCAGATCCCGGCGCTGAC
GGCGATCGGCGCGCCCGCGGTCTGCGCCGAACTGGCCTTGCGCCCGCGCGGCCTGGTGCTGGTGACCGGCGCCACCGGTT
CAGGCAAGAGCACCACGCTGGCGGCGATGGTCGGGCACATCAACGAACATCGCGCAGCGCATATCATCACCGTCGAAGAC
CCGATCGAATTCATCCACGAGCCGCGCCGCTCGCTGGTCAGCCAGCGTGAACTGGGCGCGCACACGCGCTCCTTCGCCGA
CGCCCTGCGCGGCGCGCTGCGCGAAGATCCCGACGTACTGCTGGTGGGTGAACTGCGCGACGCCGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACGGCGTCCGCGGCCAAGACGGTGGACCGCATC
GTCGACGTCTTTGCGCCCGGCGAAAAGGACGGCGCGCGCGCCATGCTGGCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGCCCGCGCCCGACGGCCACAGCCGCGTGGCCGCGCACGAGGTCATGCTGTGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGGCAAGACGGCGCAGCTCTACTCGGCCATCCAGACCGCCACCGGCCTGGGCATGCAGACGCTGGAGGGCTGC
CTGGGCGAGCTGGTGCATGCCGAACGCATCACGCTGGAGACCGCGCGCGCCCATGCGCGCTCGCCCGACTTCTTCCCCAT
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A246WMF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

61.159

99.424

0.608
  pilT Pseudomonas aeruginosa PAK

59.71

99.424

0.594
  pilT Acinetobacter baumannii D1279779

59.42

99.424

0.591
  pilT Acinetobacter baumannii strain A118

59.42

99.424

0.591
  pilT Acinetobacter nosocomialis M2

59.42

99.424

0.591
  pilT Pseudomonas stutzeri DSM 10701

58.841

99.424

0.585
  pilT Neisseria meningitidis 8013

58.892

98.847

0.582
  pilT Neisseria gonorrhoeae MS11

58.601

98.847

0.579
  pilT Legionella pneumophila strain Lp02

58.261

99.424

0.579
  pilT Legionella pneumophila strain ERS1305867

58.261

99.424

0.579
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

58.769

93.66

0.55
  pilT Vibrio cholerae strain A1552

58.769

93.66

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.826

99.424

0.476
  pilU Pseudomonas stutzeri DSM 10701

42.566

98.847

0.421
  pilU Acinetobacter baylyi ADP1

40.988

99.135

0.406
  pilU Vibrio cholerae strain A1552

40.233

98.847

0.398

Multiple sequence alignment