Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM329_RS22620 Genome accession   NZ_CP018814
Coordinates   4551817..4552797 (+) Length   326 a.a.
NCBI ID   WP_003860028.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. AR_0002     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4546817..4557797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM329_RS22580 (AM329_21845) - 4546893..4547219 (+) 327 WP_003862421.1 YggL family protein -
  AM329_RS22585 (AM329_21850) - 4547277..4547993 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  AM329_RS22590 (AM329_21855) - 4548060..4548422 (-) 363 WP_022651832.1 endonuclease domain-containing protein -
  AM329_RS22595 (AM329_21860) hemW 4548479..4549627 (-) 1149 WP_032610104.1 radical SAM family heme chaperone HemW -
  AM329_RS22600 (AM329_21865) - 4549620..4550213 (-) 594 WP_032610103.1 XTP/dITP diphosphatase -
  AM329_RS22605 (AM329_21870) yggU 4550217..4550513 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  AM329_RS22610 (AM329_21875) - 4550510..4551076 (-) 567 WP_003860024.1 YggT family protein -
  AM329_RS22615 (AM329_21880) - 4551098..4551799 (-) 702 WP_032610102.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM329_RS22620 (AM329_21885) pilT 4551817..4552797 (+) 981 WP_003860028.1 type IV pilus twitching motility protein PilT Machinery gene
  AM329_RS22625 (AM329_21890) ruvX 4552808..4553224 (-) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  AM329_RS22630 (AM329_21895) - 4553224..4553784 (-) 561 WP_022649353.1 YqgE/AlgH family protein -
  AM329_RS22635 (AM329_21900) gshB 4553859..4554806 (-) 948 WP_003860032.1 glutathione synthase -
  AM329_RS22640 (AM329_21905) rsmE 4554838..4555569 (-) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM329_RS22645 (AM329_21910) endA 4555621..4556328 (-) 708 WP_003860034.1 deoxyribonuclease I -
  AM329_RS22650 (AM329_21915) - 4556423..4556920 (-) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35622.60 Da        Isoelectric Point: 6.2335

>NTDB_id=210564 AM329_RS22620 WP_003860028.1 4551817..4552797(+) (pilT) [Enterobacter cloacae complex sp. AR_0002]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=210564 AM329_RS22620 WP_003860028.1 4551817..4552797(+) (pilT) [Enterobacter cloacae complex sp. AR_0002]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCTCCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCTCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment