Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   BI317_RS18160 Genome accession   NZ_CP018731
Coordinates   3968787..3969194 (-) Length   135 a.a.
NCBI ID   WP_074058081.1    Uniprot ID   -
Organism   Xanthomonas hortorum pv. gardneri strain ICMP 7383     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3963787..3974194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI317_RS18150 (BI317_18150) pilR 3965095..3966549 (+) 1455 WP_174555994.1 sigma-54 dependent transcriptional regulator Regulator
  BI317_RS18155 (BI317_18155) pilB 3966874..3968610 (-) 1737 WP_074058080.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BI317_RS18160 (BI317_18160) pilA/pilAI 3968787..3969194 (-) 408 WP_074058081.1 pilin Machinery gene
  BI317_RS18165 (BI317_18165) pilC 3969557..3970813 (+) 1257 WP_074058082.1 type II secretion system F family protein Machinery gene
  BI317_RS18170 (BI317_18170) - 3970821..3971684 (+) 864 WP_002812278.1 A24 family peptidase -
  BI317_RS18175 (BI317_18175) coaE 3971698..3972321 (+) 624 WP_074058083.1 dephospho-CoA kinase -
  BI317_RS18180 (BI317_18180) - 3972855..3973256 (+) 402 WP_074058084.1 SymE family type I addiction module toxin -
  BI317_RS18185 (BI317_18185) - 3973354..3973539 (+) 186 WP_006448358.1 hypothetical protein -
  BI317_RS18190 (BI317_18190) - 3973542..3973838 (+) 297 WP_225306997.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13943.99 Da        Isoelectric Point: 8.4717

>NTDB_id=209918 BI317_RS18160 WP_074058081.1 3968787..3969194(-) (pilA/pilAI) [Xanthomonas hortorum pv. gardneri strain ICMP 7383]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYVVKSQAASALAEITPGKVGFEQATNEGKTPSTAAADAGYIGVGGTTS
YCTVTVTATTIVCATRGGNATKFNGKNLTWTRDAATGLWSCGSDLDAKYKPGKCT

Nucleotide


Download         Length: 408 bp        

>NTDB_id=209918 BI317_RS18160 WP_074058081.1 3968787..3969194(-) (pilA/pilAI) [Xanthomonas hortorum pv. gardneri strain ICMP 7383]
ATGAAGAAGCAACAAGGTTTTACGCTGATCGAACTGATGATCGTTGTGGCGATTATCGCAATTCTGGCTGCTATCGCGCT
GCCTGCGTACCAGGACTACGTGGTCAAGTCGCAAGCAGCATCTGCTCTTGCTGAGATCACTCCCGGAAAGGTTGGCTTCG
AGCAGGCGACTAACGAGGGCAAGACACCAAGCACTGCTGCGGCTGATGCAGGCTACATTGGTGTTGGCGGTACTACCTCC
TACTGCACCGTAACTGTGACCGCTACTACCATCGTCTGCGCTACAAGAGGCGGGAATGCAACAAAATTCAACGGCAAGAA
TTTGACCTGGACTCGTGATGCTGCGACTGGTCTTTGGAGCTGCGGGTCTGATCTCGATGCTAAGTACAAGCCCGGTAAGT
GCACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

68.148

100

0.681
  pilA/pilAII Pseudomonas stutzeri DSM 10701

62.406

98.519

0.615
  pilA Pseudomonas aeruginosa PAK

44.667

100

0.496
  pilA Acinetobacter baumannii strain A118

44.755

100

0.474
  pilA Vibrio parahaemolyticus RIMD 2210633

44.697

97.778

0.437
  comP Acinetobacter baylyi ADP1

40.136

100

0.437
  pilA Ralstonia pseudosolanacearum GMI1000

35.443

100

0.415
  pilE Neisseria gonorrhoeae MS11

35.032

100

0.407
  pilA Vibrio cholerae C6706

38.028

100

0.4
  pilA Vibrio cholerae strain A1552

38.028

100

0.4
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.028

100

0.4
  pilA2 Legionella pneumophila str. Paris

38.686

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

38.686

100

0.393

Multiple sequence alignment