Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BI317_RS18155 Genome accession   NZ_CP018731
Coordinates   3966874..3968610 (-) Length   578 a.a.
NCBI ID   WP_074058080.1    Uniprot ID   -
Organism   Xanthomonas hortorum pv. gardneri strain ICMP 7383     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3961874..3973610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI317_RS18140 (BI317_18140) sucC 3961994..3963163 (-) 1170 WP_006450728.1 ADP-forming succinate--CoA ligase subunit beta -
  BI317_RS18145 (BI317_18145) - 3963396..3965009 (+) 1614 WP_006448368.1 HAMP domain-containing sensor histidine kinase -
  BI317_RS18150 (BI317_18150) pilR 3965095..3966549 (+) 1455 WP_174555994.1 sigma-54 dependent transcriptional regulator Regulator
  BI317_RS18155 (BI317_18155) pilB 3966874..3968610 (-) 1737 WP_074058080.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BI317_RS18160 (BI317_18160) pilA/pilAI 3968787..3969194 (-) 408 WP_074058081.1 pilin Machinery gene
  BI317_RS18165 (BI317_18165) pilC 3969557..3970813 (+) 1257 WP_074058082.1 type II secretion system F family protein Machinery gene
  BI317_RS18170 (BI317_18170) - 3970821..3971684 (+) 864 WP_002812278.1 A24 family peptidase -
  BI317_RS18175 (BI317_18175) coaE 3971698..3972321 (+) 624 WP_074058083.1 dephospho-CoA kinase -
  BI317_RS18180 (BI317_18180) - 3972855..3973256 (+) 402 WP_074058084.1 SymE family type I addiction module toxin -
  BI317_RS18185 (BI317_18185) - 3973354..3973539 (+) 186 WP_006448358.1 hypothetical protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62751.97 Da        Isoelectric Point: 5.4464

>NTDB_id=209917 BI317_RS18155 WP_074058080.1 3966874..3968610(-) (pilB) [Xanthomonas hortorum pv. gardneri strain ICMP 7383]
MSLTGTANLVGITGIARRLVQDGVLDEALARVAMDQAASAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
DDDDDMGDLDVSSGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLP
EHALLAEGFTAEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=209917 BI317_RS18155 WP_074058080.1 3966874..3968610(-) (pilB) [Xanthomonas hortorum pv. gardneri strain ICMP 7383]
ATGAGTCTTACGGGAACAGCTAATTTGGTTGGAATAACTGGGATTGCTAGACGTCTCGTTCAGGACGGAGTGCTTGACGA
AGCATTAGCCCGTGTTGCTATGGATCAGGCGGCATCTGCGAAAGTTCCGTTGCCTCAGTGGTTTGCGGAAAAAAAGCTAG
TCACTGCCTCCCAACTCGCCGCCGCCAATGCGGTTGAGTTCGGCATGCCACTGATGGACGTGTCGGTATTCGACGCCAGC
CAGAACGCAGTCAAGCTGGTCAGCGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCAACCCAGACCCGAGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTGGAGCCGA
TTCTGGTCGATGAGGACCAGATCCGCCGCACATTGGAGCAGTGGCAGGCAAGCAATGCATCGCTAGGCTCGTCGCTCGGT
GACGATGATGACGATATGGGGGATCTGGACGTCTCCTCCGGGGACGAGGACATGGGCGCCGGTGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCTTCCGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGGATCGACGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGTTGAGCCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGTATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAGAAGCTGGGCTATGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCATAAGCCTTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCTTGAACGACGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GTGTGAATCAGGTGCAGCAGAACGTGAAGCGCGGCATGACGTTTGCTGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCAATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATTGCACCGTACAACATCA
CTTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGGCGCCGCTGCCC
GAGCACGCGCTGTTGGCCGAGGGGTTCACCGCTGAGCAGATCGCTGCCGGAATCGAACTGTACGAGGCGGTTGGTTGCGA
CGAATGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATTGGCGCGATCGTGC
TGGAAGGCGGTAATGCAATGCAGATCGCAGAGGCCGCGCAGAGGATCGGCATCCGCGATTTGCGGCAGTCGGCGCTGGTG
AAGGCGGCGCATGGAGTGACGAGCCTGGCTGAGATCAATCGGGTGACAAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

54.577

98.27

0.536
  pilB Legionella pneumophila strain ERS1305867

52.197

98.443

0.514
  pilB Vibrio cholerae strain A1552

48.517

99.135

0.481
  pilF Neisseria gonorrhoeae MS11

48.239

98.27

0.474
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

97.578

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

35.599

100

0.381
  pilF Thermus thermophilus HB27

40.691

90.138

0.367

Multiple sequence alignment