Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CNQ48_RS04495 Genome accession   NZ_CP023386
Coordinates   893214..894194 (+) Length   326 a.a.
NCBI ID   WP_001604836.1    Uniprot ID   -
Organism   Escherichia coli strain 1190     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 888214..899194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNQ48_RS04465 (CNQ48_04465) - 888591..889577 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  CNQ48_RS04470 (CNQ48_04470) hemW 889891..891027 (-) 1137 WP_000239961.1 radical SAM family heme chaperone HemW -
  CNQ48_RS04475 (CNQ48_04475) rdgB 891020..891613 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  CNQ48_RS04480 (CNQ48_04480) yggU 891621..891911 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  CNQ48_RS04485 (CNQ48_04485) yggT 891908..892474 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  CNQ48_RS04490 (CNQ48_04490) yggS 892492..893196 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  CNQ48_RS04495 (CNQ48_04495) pilT 893214..894194 (+) 981 WP_001604836.1 type IV pilus twitching motility protein PilT Machinery gene
  CNQ48_RS04500 (CNQ48_04500) ruvX 894309..894725 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  CNQ48_RS04505 (CNQ48_04505) yqgE 894725..895288 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  CNQ48_RS04510 (CNQ48_04510) gshB 895397..896347 (-) 951 WP_000593273.1 glutathione synthase -
  CNQ48_RS04515 (CNQ48_04515) rsmE 896360..897091 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CNQ48_RS04520 (CNQ48_04520) endA 897171..897878 (-) 708 WP_000286497.1 deoxyribonuclease I -
  CNQ48_RS04525 (CNQ48_04525) yggI 897973..898470 (-) 498 WP_001296359.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36033.27 Da        Isoelectric Point: 5.7980

>NTDB_id=206776 CNQ48_RS04495 WP_001604836.1 893214..894194(+) (pilT) [Escherichia coli strain 1190]
MYMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=206776 CNQ48_RS04495 WP_001604836.1 893214..894194(+) (pilT) [Escherichia coli strain 1190]
ATGTATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCATTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTGGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.405

96.933

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.125

98.16

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment