Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BRW62_RS07745 Genome accession   NZ_CP018092
Coordinates   1540730..1541827 (-) Length   365 a.a.
NCBI ID   WP_099798965.1    Uniprot ID   A0A2D2Q265
Organism   Parathermosynechococcus lividus PCC 6715     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1535730..1546827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRW62_RS07715 (BRW62_07465) sixA 1535900..1536418 (-) 519 WP_099798960.1 phosphohistidine phosphatase SixA -
  BRW62_RS07720 (BRW62_07470) - 1536461..1537720 (-) 1260 WP_099798961.1 bifunctional oligoribonuclease/PAP phosphatase NrnA -
  BRW62_RS14635 - 1537846..1538400 (+) 555 WP_250644928.1 ChaN family lipoprotein -
  BRW62_RS14640 - 1538433..1538738 (+) 306 WP_338064277.1 ChaN family lipoprotein -
  BRW62_RS07730 (BRW62_07480) - 1538735..1539007 (-) 273 WP_099798962.1 DUF3143 domain-containing protein -
  BRW62_RS07735 (BRW62_07485) - 1539009..1539488 (-) 480 WP_227517309.1 J domain-containing protein -
  BRW62_RS07740 (BRW62_07490) - 1539472..1540692 (-) 1221 WP_099798964.1 type II secretion system F family protein -
  BRW62_RS07745 (BRW62_07495) pilT 1540730..1541827 (-) 1098 WP_099798965.1 type IV pilus twitching motility protein PilT Machinery gene
  BRW62_RS07750 (BRW62_07500) pilB/pilB1 1541887..1543908 (-) 2022 WP_099798966.1 GspE/PulE family protein Machinery gene
  BRW62_RS07755 (BRW62_07505) - 1544433..1544861 (-) 429 WP_099798967.1 hypothetical protein -
  BRW62_RS07760 (BRW62_07510) - 1544867..1545502 (-) 636 WP_099798968.1 ParA family protein -
  BRW62_RS07765 (BRW62_07515) pheS 1545512..1546522 (-) 1011 WP_099798969.1 phenylalanine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40391.39 Da        Isoelectric Point: 6.6505

>NTDB_id=205698 BRW62_RS07745 WP_099798965.1 1540730..1541827(-) (pilT) [Parathermosynechococcus lividus PCC 6715]
MDLMIEDLMEQVVANGGSDLHISAGLPPYIRISGKLTPTDYEPLTPEQCQRLIFSMLNNTQRKNLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMSERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFSNALRAALREDPDIILVGEMRDLETIQLAISAAETGHLVFGTLHTSSASQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGIISYEAAMSKSSRQDELQRLIGTGTPAAAVR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=205698 BRW62_RS07745 WP_099798965.1 1540730..1541827(-) (pilT) [Parathermosynechococcus lividus PCC 6715]
ATGGATTTAATGATTGAAGACTTAATGGAGCAAGTGGTCGCCAATGGTGGCTCAGACTTGCACATTTCGGCAGGGCTGCC
CCCTTACATTCGCATCAGTGGTAAGTTAACCCCCACGGACTATGAACCCCTCACACCGGAGCAGTGCCAACGGCTGATCT
TCAGTATGCTCAATAACACCCAGCGCAAAAACCTAGAACAGAACTGGGAGTTGGACTGCTCCTATGGTGTGCGCGGCTTA
GCCCGTTTCCGGGTCAATGTCTATAAAGATCGCGGTACCTACGCCGCCTGCTTGCGCGCCCTGAGCTCTAAAATTCCTAC
CTTTGAGCAGCTCGGCCTACCCAACATTGTCCGCGAAATGAGTGAGCGACCCCGGGGACTAATTTTGGTGACCGGTCCTA
CAGGCTCAGGGAAAACCACCACCCTAGCGGCCATGATTGACCTGATCAATAAAACCCGTGCTGAGCATATTCTCACGATT
GAAGACCCCATTGAATTTGTCTATGAGCCGATCAAGAGCCTGATCCACCAGCGTCAAGTGGGCGAAGACACCAAGAGCTT
CTCCAATGCGTTGCGGGCGGCTCTCCGGGAAGACCCCGATATTATTCTGGTGGGTGAGATGCGGGACTTGGAAACAATTC
AGCTTGCCATTTCAGCCGCAGAAACTGGTCACTTAGTTTTTGGTACCTTGCACACCAGTTCGGCCTCGCAAACCGTTGAC
CGGATGGTGGATGTGTTCCCACCAGAGCAACAGCAACAAATTCGGGTGCAGCTCTCGAACTCCTTAGTGGCGGTCTTTAG
TCAGACCTTGGTGCCGAAAAAGAATCCCAAGCCCGGTGAATTTGGCCGCATTATGGCGCAGGAGATTATGGTGGTCACTC
CAGCCATTTCTAACCTTATTCGTGAAGGTAAAACCTCACAAATTTATTCCGCCATTCAAACGGGGGGTAAGCTAGGAATG
CAAACCCTAGAGAAGGTCTTAGCCGACTATTACCGTGCTGGGATCATTAGTTATGAAGCGGCAATGTCGAAGTCGTCACG
GCAGGATGAGTTGCAACGTCTCATTGGCACTGGCACTCCTGCTGCTGCTGTTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2D2Q265

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.718

93.425

0.521
  pilT Legionella pneumophila strain Lp02

54.493

94.521

0.515
  pilT Legionella pneumophila strain ERS1305867

54.493

94.521

0.515
  pilT Pseudomonas stutzeri DSM 10701

54.227

93.973

0.51
  pilT Acinetobacter baylyi ADP1

54.303

92.329

0.501
  pilT Acinetobacter baumannii strain A118

52.478

93.973

0.493
  pilT Acinetobacter nosocomialis M2

52.478

93.973

0.493
  pilT Acinetobacter baumannii D1279779

52.478

93.973

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

95.89

0.479
  pilT Neisseria gonorrhoeae MS11

48.711

95.616

0.466
  pilT Neisseria meningitidis 8013

48.711

95.616

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

93.151

0.466
  pilT Vibrio cholerae strain A1552

50

93.151

0.466
  pilU Pseudomonas stutzeri DSM 10701

38.873

97.26

0.378
  pilU Acinetobacter baylyi ADP1

38.395

95.616

0.367

Multiple sequence alignment