Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   BRW62_RS07750 Genome accession   NZ_CP018092
Coordinates   1541887..1543908 (-) Length   673 a.a.
NCBI ID   WP_099798966.1    Uniprot ID   A0A2D2Q295
Organism   Parathermosynechococcus lividus PCC 6715     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1536887..1548908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRW62_RS14635 - 1537846..1538400 (+) 555 WP_250644928.1 ChaN family lipoprotein -
  BRW62_RS14640 - 1538433..1538738 (+) 306 WP_338064277.1 ChaN family lipoprotein -
  BRW62_RS07730 (BRW62_07480) - 1538735..1539007 (-) 273 WP_099798962.1 DUF3143 domain-containing protein -
  BRW62_RS07735 (BRW62_07485) - 1539009..1539488 (-) 480 WP_227517309.1 J domain-containing protein -
  BRW62_RS07740 (BRW62_07490) - 1539472..1540692 (-) 1221 WP_099798964.1 type II secretion system F family protein -
  BRW62_RS07745 (BRW62_07495) pilT 1540730..1541827 (-) 1098 WP_099798965.1 type IV pilus twitching motility protein PilT Machinery gene
  BRW62_RS07750 (BRW62_07500) pilB/pilB1 1541887..1543908 (-) 2022 WP_099798966.1 GspE/PulE family protein Machinery gene
  BRW62_RS07755 (BRW62_07505) - 1544433..1544861 (-) 429 WP_099798967.1 hypothetical protein -
  BRW62_RS07760 (BRW62_07510) - 1544867..1545502 (-) 636 WP_099798968.1 ParA family protein -
  BRW62_RS07765 (BRW62_07515) pheS 1545512..1546522 (-) 1011 WP_099798969.1 phenylalanine--tRNA ligase subunit alpha -
  BRW62_RS07770 (BRW62_07520) - 1546658..1548022 (+) 1365 WP_099798970.1 CPBP family intramembrane glutamic endopeptidase -
  BRW62_RS07775 (BRW62_07525) - 1548019..1548540 (-) 522 WP_099798971.1 L,D-transpeptidase -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 74047.21 Da        Isoelectric Point: 5.8501

>NTDB_id=205699 BRW62_RS07750 WP_099798966.1 1541887..1543908(-) (pilB/pilB1) [Parathermosynechococcus lividus PCC 6715]
MVNTSSSSARKAIALRKAGNPTERSLVASGYASLEQVREAMNTARKTGKSLVVALQEITGTTMPPDVLRQYHKQQLFELK
VIYGVDCLDPELNRFPTEQIEELVNTILPVDTCRTYQAIPIAKYPNADPPYVLVAMVDPDNLQAIDNISRILRGHNFTLK
RMVITLEDYQRLIDPILNKQVAETAASKHAPAALGDINIEEDIEAIGGLDDVQEGQEVDLADALRGAEDAPIIALVNKIL
AKALNDGISDIHIEPQEEYLRVRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDFR
VNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIST
AEDPIEYSLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARL
SEMGVEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTPEELARFGLSASKGVNLTLYKANKLTPEQLQAAKASGQPVCSK
CGGVGYKGRCGVYEIMRVTERLQTLITEGAPTERIKEVAVEEGMKTLLAYSLNLVKEGVTTLEEVERVTFTDTGLESELK
AKRKSSLTCRTCGAEAQPEWLECPYCLTPRFID

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=205699 BRW62_RS07750 WP_099798966.1 1541887..1543908(-) (pilB/pilB1) [Parathermosynechococcus lividus PCC 6715]
ATGGTCAACACATCATCTTCTTCTGCCCGCAAGGCGATCGCCCTGCGGAAAGCCGGTAACCCTACTGAGCGTTCCCTTGT
TGCCTCTGGCTATGCCAGCCTTGAGCAAGTACGCGAAGCCATGAATACCGCCCGCAAAACGGGCAAATCCCTTGTGGTTG
CCTTGCAAGAGATTACTGGCACCACCATGCCACCCGATGTGTTGCGTCAGTACCACAAACAACAACTCTTTGAACTCAAG
GTCATCTACGGCGTTGACTGTCTTGATCCGGAACTCAACCGCTTTCCCACTGAGCAAATTGAAGAGTTAGTGAATACAAT
TTTGCCGGTAGATACCTGCCGAACCTATCAAGCTATTCCCATTGCCAAGTACCCCAACGCCGATCCCCCCTATGTACTCG
TGGCAATGGTCGATCCCGACAACCTACAAGCCATCGATAACATCAGTCGTATTCTCCGCGGCCATAACTTTACCTTGAAG
CGGATGGTGATTACCCTTGAAGACTACCAGCGACTCATTGATCCAATTCTCAACAAGCAAGTAGCAGAAACTGCTGCCAG
CAAACATGCTCCAGCCGCCCTAGGTGATATTAACATCGAAGAAGACATTGAGGCCATTGGCGGCTTAGATGACGTTCAAG
AGGGTCAAGAAGTTGACCTTGCCGATGCCCTACGGGGTGCTGAGGATGCGCCAATCATTGCCCTTGTCAATAAAATTCTG
GCTAAAGCCCTCAACGACGGGATTTCCGATATTCACATCGAACCCCAAGAAGAGTACCTGCGGGTGCGCTTCCGTAAAGA
TGGCGTTCTCCATCAGGCCTTTGACCCTCTTCCTAAAAAAATTGTGCCTGCGGTCGTCTCGCGCTTCAAGATTTTGGCGG
ATCTCGACATTGCCGAGCGTCGTGCCCCCCAAGATGGCCGCATCCGCAAAATGTTCCAAGGTCGCCGCGTGGACTTTCGG
GTCAACACCCTGCCCAGCCGCTGGGGTGAAAAGGTCGTCCTGCGGATTCTGGATAACTCAGCCACCCAATTGGGGCTTGA
CAAACTCATTACCGATCCTGAAAGCCTAGCAATTGTCCGCGAAATGACGAAGCGACCCTTCGGCTTGATCTTGGTGACGG
GGCCCACAGGGTCTGGGAAAACCACCACCCTCTACTCTGCCCTAGCGGAGTGTAATAGTCCGGGGGTAAACATCAGCACC
GCTGAAGACCCCATTGAATACTCCCTGCCCGGCCTAACGCAGGTACAGGTCATCCGTGAAAAAGGGATGGACTTTGCCTC
CATTCTGCGTGCCTTCCTCCGTCAGGATCCGGATGTCATTCTGGTGGGGGAAACCCGCGACAAAGAAACCGCTAAAACCG
CCATTGAAGCAGCCTTGACTGGTCACTTAGTCTTGACAACGCTGCACACCAATGATGCAGCCAGTGCAGTGGCGCGCCTC
TCGGAAATGGGTGTAGAGCCTTTTATGGTTTCAGCGTCCTTGATTGGCGTGGTCGCCCAGCGGCTGATGCGGCGGGTGTG
CTCAGAATGCCGTATTCCCTACACCCCTACTCCCGAAGAGCTAGCGCGGTTTGGTCTATCGGCCTCCAAAGGAGTGAATC
TGACACTCTATAAAGCCAATAAACTCACTCCCGAGCAACTCCAAGCCGCCAAAGCCAGTGGCCAGCCAGTCTGCAGCAAG
TGTGGTGGAGTTGGTTACAAAGGCCGCTGCGGGGTCTATGAAATTATGCGGGTTACCGAACGCCTACAGACCCTAATTAC
CGAAGGTGCCCCTACCGAGCGCATTAAAGAAGTGGCTGTCGAAGAGGGGATGAAAACCCTCTTGGCCTATAGCCTAAACC
TTGTCAAAGAGGGGGTAACCACCCTTGAAGAAGTGGAGCGGGTAACCTTTACAGATACCGGTCTCGAATCAGAACTCAAA
GCAAAACGTAAAAGTTCATTAACTTGCCGTACCTGTGGTGCCGAGGCTCAGCCCGAATGGTTAGAATGCCCCTATTGCCT
GACGCCCCGCTTTATCGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(238-505)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2D2Q295

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.636

100

0.646

Multiple sequence alignment