Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BOP93_RS25345 Genome accession   NZ_CP018049
Coordinates   5594002..5595036 (-) Length   344 a.a.
NCBI ID   WP_104505014.1    Uniprot ID   A0A2L0S2T9
Organism   Pseudomonas orientalis strain F9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5589002..5600036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BOP93_RS25315 (BOP93_24520) ruvX 5589088..5589525 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  BOP93_RS25320 (BOP93_24525) pyrR 5589673..5590179 (+) 507 WP_065891571.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  BOP93_RS25325 (BOP93_24530) - 5590204..5591208 (+) 1005 WP_057723520.1 aspartate carbamoyltransferase catalytic subunit -
  BOP93_RS25330 (BOP93_24535) - 5591205..5592476 (+) 1272 WP_065891572.1 dihydroorotase -
  BOP93_RS25335 (BOP93_24540) - 5592549..5592977 (-) 429 WP_065896144.1 TM2 domain-containing protein -
  BOP93_RS25340 (BOP93_24545) - 5593227..5593865 (+) 639 WP_065885353.1 C40 family peptidase -
  BOP93_RS25345 (BOP93_24550) pilT 5594002..5595036 (-) 1035 WP_104505014.1 type IV pilus twitching motility protein PilT Machinery gene
  BOP93_RS25350 (BOP93_24555) - 5595094..5595780 (+) 687 WP_104505015.1 YggS family pyridoxal phosphate-dependent enzyme -
  BOP93_RS25355 (BOP93_24560) proC 5595814..5596632 (+) 819 WP_065885350.1 pyrroline-5-carboxylate reductase -
  BOP93_RS25360 (BOP93_24565) - 5596658..5597245 (+) 588 WP_057723513.1 YggT family protein -
  BOP93_RS25365 (BOP93_24570) - 5597450..5598592 (+) 1143 WP_065885349.1 homoserine O-acetyltransferase -
  BOP93_RS25370 (BOP93_24575) metW 5598618..5599238 (+) 621 WP_016972957.1 methionine biosynthesis protein MetW -
  BOP93_RS25375 (BOP93_24580) - 5599260..5599694 (+) 435 WP_065891576.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37394.14 Da        Isoelectric Point: 6.8968

>NTDB_id=205366 BOP93_RS25345 WP_104505014.1 5594002..5595036(-) (pilT) [Pseudomonas orientalis strain F9]
MDITELLTASVRRGASDLHLSAGLAPMLRIDGEVWPLDGPVLAGSQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRVNVFQQARGVGAVFRTIPSDIQSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAALIDYLNRHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRL
VDVFPGGEKAMVRSMLSESLQAVVSQVLVRKIGGGRVAAHEIMLGTPAIRHLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVSEGVISQEEAREKARVPADL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=205366 BOP93_RS25345 WP_104505014.1 5594002..5595036(-) (pilT) [Pseudomonas orientalis strain F9]
ATGGATATCACTGAATTACTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCACCTGTCGGCCGGCCTGGCGCCGAT
GTTGCGCATTGATGGCGAGGTCTGGCCGCTGGATGGCCCGGTGCTGGCCGGGTCGCAGGTAGCGGATTTATTGAGCCCTT
TGCTCAACCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACAGACTTTGCCTTCGAACTGCCCGGCGTGGCGCGC
TTTCGCGTGAATGTGTTCCAGCAGGCGCGGGGCGTGGGGGCGGTGTTTCGCACCATCCCCAGCGACATCCAGAGCCTGGA
AAGCCTTGGCCTGGGTGAGGTGTTCCAGCGTATCGCGCAGCTGCCGCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGCT
CGGGCAAGTCCACTACTTTGGCGGCGCTGATCGATTATCTGAATCGGCATCGACGCCAGCACATCCTTACGCTGGAAGAC
CCCATCGAATTTATCCACCGGCCGAAAATGGCGCTGATCAACCAGCGCCAGGTGCATCGCGATACCCACAGCTTCTCGGT
GGCGCTGCGCTCGGCATTGCGCGAAGACCCGGACGTCATCCTGGTGGGCGAGCTACGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCTGAGACGGGGCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCAAAGACGGTAGACCGGCTG
GTGGACGTGTTCCCGGGCGGGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGGTGTCCCAGGT
GCTGGTCAGGAAGATCGGCGGCGGACGTGTCGCGGCTCACGAAATCATGCTGGGCACGCCGGCCATTCGTCATCTGATCC
GCGAAGACAAAGTGGCGCAGATGGTCTCGGCGATCCAGACCGGCGGGGCGCTGGGGATGAAAACGCTGGATATGAGCTTG
AAGGCGCTGGTGAGCGAGGGGGTAATCAGCCAAGAGGAGGCGCGGGAGAAGGCGAGGGTGCCGGCAGACCTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0S2T9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.674

100

0.727
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter nosocomialis M2

69.795

99.128

0.692
  pilT Acinetobacter baumannii D1279779

69.501

99.128

0.689
  pilT Acinetobacter baumannii strain A118

69.501

99.128

0.689
  pilT Acinetobacter baylyi ADP1

68.915

99.128

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.965

0.645
  pilT Vibrio cholerae strain A1552

65.875

97.965

0.645
  pilT Legionella pneumophila strain Lp02

63.905

98.256

0.628
  pilT Legionella pneumophila strain ERS1305867

63.905

98.256

0.628
  pilT Neisseria meningitidis 8013

61.176

98.837

0.605
  pilT Neisseria gonorrhoeae MS11

60.882

98.837

0.602
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392

Multiple sequence alignment