Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WM95_RS20535 Genome accession   NZ_CP017990
Coordinates   4156160..4157140 (-) Length   326 a.a.
NCBI ID   WP_047743817.1    Uniprot ID   A0A1Z3MZT9
Organism   Enterobacter cloacae complex sp. ECNIH7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4151160..4162140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM95_RS20505 (WM95_19975) - 4152036..4152533 (+) 498 WP_045888327.1 SprT family zinc-dependent metalloprotease -
  WM95_RS20510 (WM95_19980) endA 4152628..4153335 (+) 708 WP_063409118.1 deoxyribonuclease I -
  WM95_RS20515 (WM95_19985) rsmE 4153387..4154118 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WM95_RS20520 (WM95_19990) gshB 4154138..4155085 (+) 948 WP_029739222.1 glutathione synthase -
  WM95_RS20525 (WM95_19995) - 4155173..4155733 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  WM95_RS20530 (WM95_20000) ruvX 4155733..4156149 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  WM95_RS20535 (WM95_20005) pilT 4156160..4157140 (-) 981 WP_047743817.1 type IV pilus twitching motility protein PilT Machinery gene
  WM95_RS20540 (WM95_20010) - 4157158..4157859 (+) 702 WP_063409117.1 YggS family pyridoxal phosphate-dependent enzyme -
  WM95_RS20545 (WM95_20015) - 4157881..4158447 (+) 567 WP_063409116.1 YggT family protein -
  WM95_RS20550 (WM95_20020) yggU 4158444..4158740 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  WM95_RS20555 (WM95_20025) - 4158744..4159337 (+) 594 WP_063409115.1 XTP/dITP diphosphatase -
  WM95_RS20560 (WM95_20030) hemW 4159330..4160472 (+) 1143 WP_023309133.1 radical SAM family heme chaperone HemW -
  WM95_RS20565 (WM95_20035) - 4160536..4160874 (+) 339 WP_023309134.1 endonuclease domain-containing protein -
  WM95_RS20570 (WM95_20040) - 4160957..4161673 (-) 717 WP_023309135.1 DUF2884 domain-containing protein -
  WM95_RS20575 (WM95_20045) - 4161730..4162056 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35519.56 Da        Isoelectric Point: 6.9108

>NTDB_id=204879 WM95_RS20535 WP_047743817.1 4156160..4157140(-) (pilT) [Enterobacter cloacae complex sp. ECNIH7]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=204879 WM95_RS20535 WP_047743817.1 4156160..4157140(-) (pilT) [Enterobacter cloacae complex sp. ECNIH7]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGTCGGCTTGAGCCTGCGCCTTTTCCGCCTCCCGATGTCGGAGCGTTATTGAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATCTGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTTACGCTGCGGCTGCTGCCGCGTAGCTGCCCACAGCTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTGACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGTCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACACCTGGTACTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCTGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGCCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3MZT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment