Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BJK05_RS12665 Genome accession   NZ_CP017482
Coordinates   2789897..2790913 (-) Length   338 a.a.
NCBI ID   WP_095700495.1    Uniprot ID   -
Organism   Pectobacterium polaris strain NIBIO1392     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2784897..2795913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJK05_RS12650 (BJK05_12220) nsrR 2785246..2785671 (-) 426 WP_005974824.1 nitric oxide-sensing transcriptional repressor NsrR -
  BJK05_RS12655 (BJK05_12225) - 2785999..2787570 (+) 1572 WP_095700493.1 methyl-accepting chemotaxis protein -
  BJK05_RS12660 (BJK05_12230) - 2788096..2789928 (+) 1833 WP_095700494.1 diguanylate cyclase -
  BJK05_RS12665 (BJK05_12235) pilT 2789897..2790913 (-) 1017 WP_095700495.1 type IV pilus twitching motility protein PilT Machinery gene
  BJK05_RS12670 (BJK05_12240) - 2790934..2791647 (+) 714 WP_095700496.1 YggS family pyridoxal phosphate-dependent enzyme -
  BJK05_RS12675 (BJK05_12245) proC 2791723..2792544 (+) 822 WP_095701961.1 pyrroline-5-carboxylate reductase -
  BJK05_RS12680 (BJK05_12250) - 2792594..2793148 (+) 555 WP_039486302.1 YggT family protein -
  BJK05_RS12685 (BJK05_12255) yggU 2793145..2793435 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  BJK05_RS12690 (BJK05_12260) - 2793453..2794046 (+) 594 WP_095700497.1 XTP/dITP diphosphatase -
  BJK05_RS12695 (BJK05_12265) hemW 2794039..2795178 (+) 1140 WP_095700498.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36758.97 Da        Isoelectric Point: 6.5682

>NTDB_id=198693 BJK05_RS12665 WP_095700495.1 2789897..2790913(-) (pilT) [Pectobacterium polaris strain NIBIO1392]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLEALHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSVSFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLSAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=198693 BJK05_RS12665 WP_095700495.1 2789897..2790913(-) (pilT) [Pectobacterium polaris strain NIBIO1392]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCAGT
GTTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGCGCGTTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGTGAAGGGCTGTCTGCGGCTCTGCGTATTATCCCGTCCGCTCAGCCTTCATT
GGAAGCGCTACATGCGCCACCCGTTCTATCGACGCTGCTGGAGAAGCCGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAATGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGTCGACGCTGCCTGATTCAGCAGCGGGAGATTGGTGCGCACAGTGTGTCGTTTGC
GCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACTGCGGCGGAAACCGGTCATTTGGTGTTGTCGACGCTGCATACACGCAGTGCATCGCAGGCGGTTGATCGT
TTGATTGATGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGCTAGCTACCTGTTTGCAGGCGGTGGTGACGCA
AAGGCTGTTGTCCGCTGCGCAAGGTGGCCGAATCGCGCTTTATGAAGTGCTGACGGCGACTGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.904

98.225

0.5
  pilT Neisseria gonorrhoeae MS11

50.602

98.225

0.497
  pilT Pseudomonas stutzeri DSM 10701

49.547

97.929

0.485
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Legionella pneumophila strain Lp02

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.84

99.704

0.417
  pilU Vibrio cholerae strain A1552

39.359

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.24

98.521

0.396
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment