Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BJJ97_RS00975 Genome accession   NZ_CP017481
Coordinates   219430..220446 (-) Length   338 a.a.
NCBI ID   WP_095992816.1    Uniprot ID   -
Organism   Pectobacterium polaris strain NIBIO1006     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214430..225446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJJ97_RS00960 (BJJ97_00940) nsrR 214777..215202 (-) 426 WP_005974824.1 nitric oxide-sensing transcriptional repressor NsrR -
  BJJ97_RS00965 (BJJ97_00945) - 215530..217101 (+) 1572 WP_095992814.1 methyl-accepting chemotaxis protein -
  BJJ97_RS00970 (BJJ97_00950) - 217629..219461 (+) 1833 WP_095992815.1 diguanylate cyclase -
  BJJ97_RS00975 (BJJ97_00955) pilT 219430..220446 (-) 1017 WP_095992816.1 type IV pilus twitching motility protein PilT Machinery gene
  BJJ97_RS00980 (BJJ97_00960) - 220467..221180 (+) 714 WP_095992817.1 YggS family pyridoxal phosphate-dependent enzyme -
  BJJ97_RS00985 (BJJ97_00965) proC 221297..222118 (+) 822 WP_095995300.1 pyrroline-5-carboxylate reductase -
  BJJ97_RS00990 (BJJ97_00970) - 222168..222722 (+) 555 WP_039486302.1 YggT family protein -
  BJJ97_RS00995 (BJJ97_00975) yggU 222719..223009 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  BJJ97_RS01000 (BJJ97_00980) - 223027..223620 (+) 594 WP_095992818.1 XTP/dITP diphosphatase -
  BJJ97_RS01005 (BJJ97_00985) hemW 223613..224755 (+) 1143 WP_095992819.1 radical SAM family heme chaperone HemW -
  BJJ97_RS01010 - 224985..225245 (+) 261 WP_095995301.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36747.98 Da        Isoelectric Point: 6.4958

>NTDB_id=198660 BJJ97_RS00975 WP_095992816.1 219430..220446(-) (pilT) [Pectobacterium polaris strain NIBIO1006]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLEALQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSSSFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAVQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=198660 BJJ97_RS00975 WP_095992816.1 219430..220446(-) (pilT) [Pectobacterium polaris strain NIBIO1006]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGCGCGTTAATGCTGCCCGATGGGCAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGTTGTCTGCGGCGCTGCGCATTATCCCGTCCGCTCAGCCTTCATT
GGAAGCGCTACAGGCGCCACCCGTTCTATCGACGCTGCTGGAGAAACCGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGTGATCGCCATGTGATTACGCTGGAA
GACCCGATCGAGTTTATCCATGCCAGCCGGCGCTGCCTGATTCAACAGCGGGAGATTGGTGCGCACAGTTCATCGTTTGC
ACAGGCGCTACGAGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGTCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGACCGT
TTGATTGATGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCGGTGGTGACGCA
AAAATTATTACCCGCCGTGCAAGGTGGGCGAATCGCACTGTATGAAGTGCTGACTGCGACTGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

97.633

0.509
  pilT Vibrio cholerae strain A1552

52.121

97.633

0.509
  pilT Neisseria meningitidis 8013

50.904

98.225

0.5
  pilT Neisseria gonorrhoeae MS11

50.602

98.225

0.497
  pilT Pseudomonas stutzeri DSM 10701

49.849

97.929

0.488
  pilT Legionella pneumophila strain Lp02

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.84

99.704

0.417
  pilU Pseudomonas stutzeri DSM 10701

40.541

98.521

0.399
  pilU Vibrio cholerae strain A1552

39.359

100

0.399
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment