Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BHT24_RS16910 Genome accession   NZ_CP017220
Coordinates   3249493..3250473 (-) Length   326 a.a.
NCBI ID   WP_072048899.1    Uniprot ID   -
Organism   Escherichia coli strain FAM21845     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3244493..3255473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHT24_RS16880 (BHT24_16195) yggI 3245157..3245654 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  BHT24_RS16885 (BHT24_16200) endA 3245749..3246456 (+) 708 WP_081178194.1 deoxyribonuclease I -
  BHT24_RS16890 (BHT24_16205) rsmE 3246536..3247267 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BHT24_RS16895 (BHT24_16210) gshB 3247280..3248230 (+) 951 WP_040062533.1 glutathione synthase -
  BHT24_RS16900 (BHT24_16215) yqgE 3248339..3248902 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  BHT24_RS16905 (BHT24_16220) ruvX 3248902..3249318 (+) 417 WP_044863764.1 Holliday junction resolvase RuvX -
  BHT24_RS16910 (BHT24_16225) pilT 3249493..3250473 (-) 981 WP_072048899.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BHT24_RS16915 (BHT24_16230) yggS 3250491..3251195 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  BHT24_RS16920 (BHT24_16235) yggT 3251213..3251779 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  BHT24_RS16925 (BHT24_16240) yggU 3251776..3252066 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  BHT24_RS16930 (BHT24_16245) rdgB 3252074..3252667 (+) 594 WP_001174747.1 XTP/dITP diphosphatase -
  BHT24_RS16935 (BHT24_16250) hemW 3252660..3253796 (+) 1137 WP_000239924.1 radical SAM family heme chaperone HemW -
  BHT24_RS16940 (BHT24_16255) yggM 3253865..3254872 (-) 1008 WP_000745244.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35970.22 Da        Isoelectric Point: 5.9975

>NTDB_id=196651 BHT24_RS16910 WP_072048899.1 3249493..3250473(-) (pilT) [Escherichia coli strain FAM21845]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAEKQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=196651 BHT24_RS16910 WP_072048899.1 3249493..3250473(-) (pilT) [Escherichia coli strain FAM21845]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAAACAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCTCCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGAGCGACAGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTAACAG
CGGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACACGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCAGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.578

98.773

0.371

Multiple sequence alignment