Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BHS07_RS30290 Genome accession   NZ_CP017172
Coordinates   7342612..7343730 (-) Length   372 a.a.
NCBI ID   WP_140794794.1    Uniprot ID   -
Organism   Myxococcus xanthus strain KF4.3.9c1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7337612..7348730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS07_RS30275 (BHS07_29505) - 7338274..7339710 (-) 1437 WP_140799699.1 sigma-54-dependent transcriptional regulator -
  BHS07_RS30280 (BHS07_29510) - 7339707..7341239 (-) 1533 WP_140878511.1 two-component system sensor histidine kinase NtrB -
  BHS07_RS30285 (BHS07_29515) - 7341301..7342554 (-) 1254 WP_140794793.1 type II secretion system F family protein -
  BHS07_RS30290 (BHS07_29520) pilT 7342612..7343730 (-) 1119 WP_140794794.1 type IV pilus twitching motility protein PilT Machinery gene
  BHS07_RS30295 (BHS07_29525) pilB 7343774..7345474 (-) 1701 WP_011555739.1 type IV-A pilus assembly ATPase PilB -
  BHS07_RS30300 (BHS07_29530) - 7345679..7346605 (-) 927 WP_140794795.1 bifunctional riboflavin kinase/FAD synthetase -
  BHS07_RS30305 (BHS07_29535) trmB 7346681..7347301 (+) 621 WP_140876938.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  BHS07_RS30310 (BHS07_29540) - 7347413..7348456 (+) 1044 WP_140794797.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40653.03 Da        Isoelectric Point: 8.2933

>NTDB_id=196291 BHS07_RS30290 WP_140794794.1 7342612..7343730(-) (pilT) [Myxococcus xanthus strain KF4.3.9c1]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDIVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQMSFVLEGVMSQALVAKAGGPGRILALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRLITQDEAFGRSSDAEELRNILATGGGGALPGMQRPGGGAGGR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=196291 BHS07_RS30290 WP_140794794.1 7342612..7343730(-) (pilT) [Myxococcus xanthus strain KF4.3.9c1]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
GCAGCTTCGCGTCGACGGCGAGCTCGTTCCCTTGAAGACGGCGCCGCTGACCCCGGTGGAGACGAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACTTGTCCTTCGGTGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGTGGCGCGGTCGCCGCGGCGTTCCGGACCATTCCCTTCAAGATTCTGACGTT
CCAGGAGCTGGGGCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGGCTCATCCTGGTGACGGGCCCCACGG
GTTCAGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTGAATCAGCGCGAGGTGGGAGCGGACACGCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACATCGTGCTCGTCGGTGAGCTTCGCGACCTGGAGACGATTGAGG
CGGCGCTCACCATCGCCGAGACGGGTCACATCTGCTACGCGACGCTACACACCAATAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTCCGCGCGCAGATGTCCTTCGTGCTGGAAGGCGTGATGAGCCA
GGCGCTGGTCGCGAAGGCGGGCGGCCCTGGCCGCATCCTGGCGCTGGAGGTGATGGTGCCCAACCCCGCAATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTCGGCCAGGCGAAGTTCGGCATGCAGACCTTCAAC
CAGGCTCTGGCGGCGCTGCTGCTGCGGCGGCTCATCACCCAGGACGAGGCCTTCGGGCGCTCTAGCGACGCGGAGGAGCT
GCGCAACATCCTGGCCACGGGCGGCGGCGGGGCCCTGCCCGGGATGCAGCGACCAGGCGGGGGAGCGGGTGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.696

96.505

0.489
  pilT Legionella pneumophila strain ERS1305867

53.134

90.054

0.478
  pilT Legionella pneumophila strain Lp02

53.134

90.054

0.478
  pilT Pseudomonas aeruginosa PAK

52.537

90.054

0.473
  pilT Pseudomonas stutzeri DSM 10701

52.537

90.054

0.473
  pilT Acinetobacter baumannii D1279779

52.266

88.978

0.465
  pilT Acinetobacter baumannii strain A118

52.266

88.978

0.465
  pilT Acinetobacter nosocomialis M2

52.266

88.978

0.465
  pilT Acinetobacter baylyi ADP1

52.424

88.71

0.465
  pilT Vibrio cholerae strain A1552

48.204

89.785

0.433
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.204

89.785

0.433
  pilT Neisseria meningitidis 8013

44.575

91.667

0.409
  pilT Neisseria gonorrhoeae MS11

44.575

91.667

0.409
  pilU Vibrio cholerae strain A1552

43.059

94.892

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.355

0.384
  pilU Acinetobacter baylyi ADP1

39.193

93.28

0.366

Multiple sequence alignment