Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BHS06_RS27775 Genome accession   NZ_CP017171
Coordinates   6899650..6900768 (-) Length   372 a.a.
NCBI ID   WP_011555738.1    Uniprot ID   Q1D099
Organism   Myxococcus xanthus strain KF3.2.8c11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6894650..6905768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS06_RS27760 (BHS06_27220) - 6895312..6896748 (-) 1437 WP_140866016.1 sigma-54-dependent transcriptional regulator -
  BHS06_RS27765 (BHS06_27225) - 6896745..6898277 (-) 1533 WP_140871280.1 two-component system sensor histidine kinase NtrB -
  BHS06_RS27770 (BHS06_27230) - 6898339..6899592 (-) 1254 WP_140866019.1 type II secretion system F family protein -
  BHS06_RS27775 (BHS06_27235) pilT 6899650..6900768 (-) 1119 WP_011555738.1 type IV pilus twitching motility protein PilT Machinery gene
  BHS06_RS27780 (BHS06_27240) pilB 6900812..6902512 (-) 1701 WP_011555739.1 type IV-A pilus assembly ATPase PilB -
  BHS06_RS27785 (BHS06_27245) - 6902717..6903643 (-) 927 WP_140866022.1 bifunctional riboflavin kinase/FAD synthetase -
  BHS06_RS27790 (BHS06_27250) trmB 6903719..6904339 (+) 621 WP_140866025.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  BHS06_RS27795 (BHS06_27255) - 6904450..6905493 (+) 1044 WP_140866028.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40624.97 Da        Isoelectric Point: 8.2933

>NTDB_id=196279 BHS06_RS27775 WP_011555738.1 6899650..6900768(-) (pilT) [Myxococcus xanthus strain KF3.2.8c11]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQMSFVLEGVMSQALVAKAGGPGRILALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRLITQDEAFGRSSDAEELRNILATGGGGALPGMQRPGGGAGGR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=196279 BHS06_RS27775 WP_011555738.1 6899650..6900768(-) (pilT) [Myxococcus xanthus strain KF3.2.8c11]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
GCAGCTTCGCGTCGACGGCGAGCTCGTTCCCTTGAAGACGGCGCCGCTGACCCCGGTGGAGACGAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGACAACGAGCTGGACCTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGTGGCGCGGTCGCCGCGGCGTTCCGGACCATTCCCTTCAAGATCCTGACGTT
CCAGGAGCTGGGCCTTCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCCCGCGGGCTCATCCTGGTGACGGGCCCCACGG
GCTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCTATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTGAATCAGCGCGAGGTGGGGGCGGACACGCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTGGTCGGTGAGCTCCGCGACCTGGAGACGATTGAGG
CGGCGCTCACCATCGCGGAGACGGGTCACATCTGCTACGCGACGCTACACACCAATAGCGCGGTGCAGACCATCAACCGC
GTGTTGGACGTGTTCCCCCCGTACCAGCAGCCGCAAGTCCGCGCGCAGATGTCCTTCGTGCTGGAAGGCGTGATGAGTCA
GGCGCTGGTGGCGAAGGCGGGCGGCCCCGGCCGCATCCTGGCGCTGGAGGTGATGGTGCCCAACCCCGCGATCCGGAACC
TCATTCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTCGGCCAGGCGAAGTTCGGCATGCAGACCTTCAAT
CAGGCCCTGGCGGCGCTGCTGCTGCGGCGGCTCATCACCCAGGACGAGGCCTTCGGGCGTTCTAGCGACGCGGAGGAGTT
GCGCAACATCCTGGCCACGGGCGGCGGCGGGGCACTGCCCGGTATGCAGCGGCCAGGCGGGGGAGCGGGTGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1D099

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.975

96.505

0.492
  pilT Legionella pneumophila strain ERS1305867

52.836

90.054

0.476
  pilT Legionella pneumophila strain Lp02

52.836

90.054

0.476
  pilT Pseudomonas aeruginosa PAK

52.239

90.054

0.47
  pilT Pseudomonas stutzeri DSM 10701

52.239

90.054

0.47
  pilT Acinetobacter baumannii D1279779

51.964

88.978

0.462
  pilT Acinetobacter baumannii strain A118

51.964

88.978

0.462
  pilT Acinetobacter nosocomialis M2

51.964

88.978

0.462
  pilT Acinetobacter baylyi ADP1

52.121

88.71

0.462
  pilT Vibrio cholerae strain A1552

48.503

89.785

0.435
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

89.785

0.435
  pilT Neisseria meningitidis 8013

44.868

91.667

0.411
  pilT Neisseria gonorrhoeae MS11

44.868

91.667

0.411
  pilU Vibrio cholerae strain A1552

43.059

94.892

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.026

94.355

0.387
  pilU Acinetobacter baylyi ADP1

39.481

93.28

0.368

Multiple sequence alignment