Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BHS04_RS29810 Genome accession   NZ_CP017169
Coordinates   7266033..7267148 (-) Length   371 a.a.
NCBI ID   WP_140856701.1    Uniprot ID   -
Organism   Myxococcus xanthus strain GH3.5.6c2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7261033..7272148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS04_RS29795 (BHS04_29050) - 7261695..7263131 (-) 1437 WP_140856697.1 sigma-54-dependent transcriptional regulator -
  BHS04_RS29800 (BHS04_29055) - 7263128..7264660 (-) 1533 WP_140858538.1 two-component system sensor histidine kinase NtrB -
  BHS04_RS29805 (BHS04_29060) - 7264722..7265975 (-) 1254 WP_140856699.1 type II secretion system F family protein -
  BHS04_RS29810 (BHS04_29065) pilT 7266033..7267148 (-) 1116 WP_140856701.1 type IV pilus twitching motility protein PilT Machinery gene
  BHS04_RS29815 (BHS04_29070) pilB 7267192..7268892 (-) 1701 WP_011555739.1 type IV-A pilus assembly ATPase PilB -
  BHS04_RS29820 (BHS04_29075) - 7269097..7270023 (-) 927 WP_140856703.1 bifunctional riboflavin kinase/FAD synthetase -
  BHS04_RS29825 (BHS04_29080) trmB 7270099..7270719 (+) 621 WP_140794796.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  BHS04_RS29830 (BHS04_29085) - 7270831..7271874 (+) 1044 WP_140794797.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40642.00 Da        Isoelectric Point: 8.2933

>NTDB_id=196255 BHS04_RS29810 WP_140856701.1 7266033..7267148(-) (pilT) [Myxococcus xanthus strain GH3.5.6c2]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPPVVAELVKKSRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDIVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQMSFVLEGVMSQALVAKAGGPGRILALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRLITQDEAFGRSTDPEELRNILATGGGTLPGMQRPGGGAGGR

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=196255 BHS04_RS29810 WP_140856701.1 7266033..7267148(-) (pilT) [Myxococcus xanthus strain GH3.5.6c2]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
GCAGCTTCGCGTCGACGGCGAGCTCGTTCCCTTGAAGACGGCGCCGCTGACCCCGGTGGAGACGAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAAGAGGATAACGAGCTGGACCTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGTGGCGCGGTCGCCGCGGCGTTCCGGACCATTCCCTTCAAGATTCTGACGTT
CCAGGAGCTGGGGCTTCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGTCGCGCGGGCTCATCCTGGTGACGGGCCCCACGG
GCTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTGAATCAGCGCGAGGTGGGCGCGGACACGCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGACAGGACCCGGACATCGTGCTCGTCGGTGAGCTCCGCGACCTGGAGACCATCGAAG
CGGCGCTCACCATCGCTGAGACGGGTCACATCTGCTACGCAACGCTACACACCAATAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTCCGCGCGCAGATGTCCTTCGTGCTGGAGGGCGTGATGAGCCA
GGCGCTGGTGGCGAAGGCGGGCGGCCCTGGCCGCATCCTGGCGCTGGAGGTGATGGTGCCCAACCCCGCAATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTCGGCCAGGCGAAGTTCGGCATGCAGACCTTCAAC
CAGGCCCTGGCGGCGCTGCTGCTGCGGCGGCTCATCACCCAGGACGAGGCCTTCGGGCGTTCTACTGATCCTGAGGAACT
GCGCAACATCCTGGCCACGGGCGGCGGGACCCTGCCCGGGATGCAGCGACCAGGCGGGGGAGCGGGTGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.433

90.296

0.482
  pilT Legionella pneumophila strain Lp02

53.433

90.296

0.482
  pilT Pseudomonas stutzeri DSM 10701

51.304

92.992

0.477
  pilT Pseudomonas aeruginosa PAK

51.304

92.992

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

92.992

0.474
  pilT Acinetobacter baylyi ADP1

50.725

92.992

0.472
  pilT Acinetobacter baumannii D1279779

50.725

92.992

0.472
  pilT Acinetobacter baumannii strain A118

50.725

92.992

0.472
  pilT Acinetobacter nosocomialis M2

50.725

92.992

0.472
  pilT Vibrio cholerae strain A1552

48.204

90.027

0.434
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.204

90.027

0.434
  pilT Neisseria meningitidis 8013

44.282

91.914

0.407
  pilT Neisseria gonorrhoeae MS11

44.282

91.914

0.407
  pilU Vibrio cholerae strain A1552

42.776

95.148

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.678

95.418

0.388
  pilU Acinetobacter baylyi ADP1

39.481

93.531

0.369

Multiple sequence alignment