Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EnteroDNA1_RS10260 Genome accession   NZ_CP017087
Coordinates   2186415..2187395 (-) Length   326 a.a.
NCBI ID   WP_069303286.1    Uniprot ID   -
Organism   Enterobacter sp. HK169     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2181415..2192395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EnteroDNA1_RS10230 (EnteroDNA1_02034) - 2182291..2182788 (+) 498 WP_045888327.1 SprT family zinc-dependent metalloprotease -
  EnteroDNA1_RS10235 (EnteroDNA1_02035) endA 2182883..2183590 (+) 708 WP_063439816.1 deoxyribonuclease I -
  EnteroDNA1_RS10240 (EnteroDNA1_02036) rsmE 2183642..2184373 (+) 732 WP_058841908.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EnteroDNA1_RS10245 (EnteroDNA1_02037) gshB 2184393..2185340 (+) 948 WP_029739222.1 glutathione synthase -
  EnteroDNA1_RS10250 (EnteroDNA1_02038) - 2185428..2185988 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  EnteroDNA1_RS10255 (EnteroDNA1_02039) ruvX 2185988..2186404 (+) 417 WP_069303285.1 Holliday junction resolvase RuvX -
  EnteroDNA1_RS10260 (EnteroDNA1_02040) pilT 2186415..2187395 (-) 981 WP_069303286.1 type IV pilus twitching motility protein PilT Machinery gene
  EnteroDNA1_RS10265 (EnteroDNA1_02041) - 2187413..2188114 (+) 702 WP_069303287.1 YggS family pyridoxal phosphate-dependent enzyme -
  EnteroDNA1_RS10270 (EnteroDNA1_02042) - 2188136..2188702 (+) 567 WP_033146608.1 YggT family protein -
  EnteroDNA1_RS10275 (EnteroDNA1_02043) yggU 2188699..2188995 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  EnteroDNA1_RS10280 (EnteroDNA1_02044) - 2188999..2189592 (+) 594 WP_064673281.1 XTP/dITP diphosphatase -
  EnteroDNA1_RS10285 (EnteroDNA1_02045) hemW 2189585..2190727 (+) 1143 WP_064673282.1 radical SAM family heme chaperone HemW -
  EnteroDNA1_RS21830 - 2190791..2191153 (+) 363 WP_071891574.1 endonuclease domain-containing protein -
  EnteroDNA1_RS10295 (EnteroDNA1_02046) - 2191214..2191930 (-) 717 WP_069303288.1 DUF2884 domain-containing protein -
  EnteroDNA1_RS10300 (EnteroDNA1_02047) - 2191987..2192313 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35437.45 Da        Isoelectric Point: 6.6964

>NTDB_id=195358 EnteroDNA1_RS10260 WP_069303286.1 2186415..2187395(-) (pilT) [Enterobacter sp. HK169]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAKGQVDFAATVTGGQRLRG
SAFKQMSGVSLTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=195358 EnteroDNA1_RS10260 WP_069303286.1 2186415..2187395(-) (pilT) [Enterobacter sp. HK169]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGTCGGCTTGAACCCGCTCCCTTTCCGCCTCCCGATGTCGGAGCGTTATTAAAAGCATGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGTGGTGTCTCTTTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCACAGCTCTCTTCGCTGGG
CGCGCCGCGGGCTATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAATCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGTGAGATAGGCCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGACTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAGACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment