Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BFG52_RS12475 Genome accession   NZ_CP016895
Coordinates   2797146..2798147 (+) Length   333 a.a.
NCBI ID   WP_067556760.1    Uniprot ID   -
Organism   Acinetobacter larvae strain BRTC-1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2792146..2803147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFG52_RS12465 (BFG52_12465) - 2794037..2796175 (+) 2139 WP_067556754.1 TonB-dependent siderophore receptor -
  BFG52_RS12470 (BFG52_12470) - 2796344..2797030 (-) 687 WP_067556757.1 YggS family pyridoxal phosphate-dependent enzyme -
  BFG52_RS12475 (BFG52_12475) pilT 2797146..2798147 (+) 1002 WP_067556760.1 type IV pilus twitching motility protein PilT Machinery gene
  BFG52_RS12480 (BFG52_12480) fur 2798253..2798690 (-) 438 WP_067556763.1 ferric iron uptake transcriptional regulator -
  BFG52_RS12485 (BFG52_12485) - 2798803..2799201 (+) 399 WP_067556765.1 outer membrane protein assembly factor BamE -
  BFG52_RS12490 (BFG52_12490) - 2799248..2799592 (-) 345 WP_067556767.1 RnfH family protein -
  BFG52_RS12495 (BFG52_12495) - 2799589..2800662 (-) 1074 WP_067556770.1 hypothetical protein -
  BFG52_RS12500 (BFG52_12500) - 2800815..2801663 (-) 849 WP_067556773.1 class II glutamine amidotransferase -
  BFG52_RS12505 (BFG52_12505) - 2801761..2802537 (-) 777 WP_067556775.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 36818.27 Da        Isoelectric Point: 7.2414

>NTDB_id=193497 BFG52_RS12475 WP_067556760.1 2797146..2798147(+) (pilT) [Acinetobacter larvae strain BRTC-1]
MQLYDLLKLGTEQGASDLHLSAGHPAMLRIDGRLQRSSSTYLTTSHITDYLAQILSAHQLQQFQQKQEIDCAIELPELGR
FRINVFQQQRGAALACRYIATQIKSLSALGLDEIFQEICQIKQGLVLVTGATGTGKSTTLAAMLDYINQTQAAHIITLED
PIEFIHHSQNCLIQQREIHTHSQSFNHALRAALREDPDIILIGELRDLETIRLALTAAETGHLVFASLHTASAAQSIHRL
IDVFPATEKQTVRHLLAESLQAVIAQTLLKKTTAGRIAVHEIMLAIPAIRHLIRDDKISQINSCIQTGAQYGMRSLAQSH
AQLRQQGIISSAL

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=193497 BFG52_RS12475 WP_067556760.1 2797146..2798147(+) (pilT) [Acinetobacter larvae strain BRTC-1]
ATGCAACTCTATGATTTATTGAAACTAGGAACTGAGCAAGGTGCATCCGACCTGCATTTAAGCGCAGGACATCCCGCCAT
GCTTAGAATCGATGGTCGTCTGCAACGTAGCAGCTCGACCTACTTAACAACAAGCCACATCACGGACTATCTGGCACAGA
TTTTATCTGCCCATCAATTGCAACAATTCCAACAAAAACAAGAAATTGATTGCGCTATTGAACTGCCCGAATTAGGTCGT
TTTCGCATCAATGTGTTCCAGCAACAACGGGGTGCCGCCTTGGCGTGTCGCTATATTGCAACGCAGATTAAAAGTTTATC
GGCCCTGGGCTTAGATGAAATATTTCAGGAGATCTGTCAGATAAAACAAGGACTAGTATTGGTTACAGGCGCCACAGGTA
CAGGGAAATCAACCACATTGGCGGCAATGCTGGACTATATTAACCAAACACAAGCTGCACATATTATCACGCTAGAAGAT
CCCATCGAGTTTATTCATCATAGTCAGAATTGTTTAATCCAGCAAAGAGAAATCCACACACATAGTCAGAGCTTCAATCA
TGCATTACGCGCAGCGCTACGCGAAGATCCAGACATTATCTTGATTGGAGAACTACGTGACTTAGAAACCATTCGTTTGG
CATTGACTGCTGCGGAGACTGGTCATTTGGTATTTGCCAGTTTGCATACCGCCTCAGCGGCACAAAGCATTCATCGCTTA
ATCGATGTATTCCCCGCCACAGAGAAACAAACGGTACGCCACCTCTTAGCGGAATCCTTACAAGCTGTAATTGCACAGAC
CTTGCTCAAGAAAACAACTGCTGGTCGCATCGCGGTCCATGAAATCATGCTCGCCATCCCTGCAATTCGTCATCTTATCC
GTGATGATAAAATTAGCCAGATTAATTCCTGCATCCAAACTGGTGCGCAATATGGTATGCGCAGTCTGGCACAAAGTCAT
GCTCAGTTACGCCAGCAAGGCATTATCAGCAGTGCCTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

57.576

99.099

0.571
  pilT Acinetobacter nosocomialis M2

56.364

99.099

0.559
  pilT Acinetobacter baumannii D1279779

56.193

99.399

0.559
  pilT Acinetobacter baumannii strain A118

56.193

99.399

0.559
  pilT Legionella pneumophila strain Lp02

53.939

99.099

0.535
  pilT Pseudomonas stutzeri DSM 10701

53.939

99.099

0.535
  pilT Legionella pneumophila strain ERS1305867

53.939

99.099

0.535
  pilT Vibrio cholerae strain A1552

53.776

99.399

0.535
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.776

99.399

0.535
  pilT Pseudomonas aeruginosa PAK

53.636

99.099

0.532
  pilT Neisseria meningitidis 8013

52.87

99.399

0.526
  pilT Neisseria gonorrhoeae MS11

52.568

99.399

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

95.195

0.42
  pilU Acinetobacter baylyi ADP1

39.564

96.396

0.381
  pilU Pseudomonas stutzeri DSM 10701

37.764

99.399

0.375
  pilU Vibrio cholerae strain A1552

39.032

93.093

0.363

Multiple sequence alignment