Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BER93_RS13485 Genome accession   NZ_CP016833
Coordinates   2946330..2947367 (-) Length   345 a.a.
NCBI ID   WP_002802057.1    Uniprot ID   -
Organism   Xanthomonas fragariae isolate Fap29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2941330..2952367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BER93_RS13450 (BER93_13450) ruvX 2941954..2942421 (-) 468 WP_002802053.1 Holliday junction resolvase RuvX -
  BER93_RS13455 (BER93_13455) - 2942414..2942980 (-) 567 WP_002802054.1 YqgE/AlgH family protein -
  BER93_RS13465 (BER93_13465) - 2943276..2943833 (+) 558 WP_002802055.1 DNA-3-methyladenine glycosylase I -
  BER93_RS13475 (BER93_13475) - 2944034..2944933 (+) 900 Protein_2701 DUF72 domain-containing protein -
  BER93_RS13480 (BER93_13480) pilU 2945093..2946223 (-) 1131 WP_002802056.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BER93_RS13485 (BER93_13485) pilT 2946330..2947367 (-) 1038 WP_002802057.1 type IV pilus twitching motility protein PilT Machinery gene
  BER93_RS13490 (BER93_13490) - 2947721..2948413 (+) 693 WP_002802058.1 YggS family pyridoxal phosphate-dependent enzyme -
  BER93_RS13495 (BER93_13495) proC 2948471..2949325 (+) 855 WP_002802060.1 pyrroline-5-carboxylate reductase -
  BER93_RS13500 (BER93_13500) - 2949888..2950313 (+) 426 WP_040761922.1 HU family DNA-binding protein -
  BER93_RS13505 (BER93_13505) - 2950420..2951061 (+) 642 Protein_2707 M23 family metallopeptidase -
  BER93_RS13510 (BER93_13510) - 2951153..2951461 (+) 309 WP_002802064.1 DUF3861 family protein -
  BER93_RS13515 (BER93_13515) - 2951574..2952170 (+) 597 WP_002802066.1 DUF1439 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38688.34 Da        Isoelectric Point: 6.6652

>NTDB_id=192554 BER93_RS13485 WP_002802057.1 2946330..2947367(-) (pilT) [Xanthomonas fragariae isolate Fap29]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIQSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLIKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=192554 BER93_RS13485 WP_002802057.1 2946330..2947367(-) (pilT) [Xanthomonas fragariae isolate Fap29]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCGTCGGACCTGCACCTGTCTGCAGGCCTGCCGCCGAT
GATCCGAGTTGATGGCGATGTCCGCCGCATCAATATTCCGGCCCTGGATCACAAGCAGGTGCATGCGCTGGTCTACGACA
TTATGTCGGACAAGCAGCGCCGCGATTACGAAGAATTCCTCGAAGTCGACTTCTCGTTCGAGATCCAGTCGCTGGCACGC
TTCCGCGTCAATGCGTTCAACCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTTGGCTGCCCACCGATTTTCCGGCAGCTGATCGATCAGCCGCAGGGCTTGATCCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACTGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCATCGCGACACGCACGGCTTCAACGA
AGCATTGCGCTCTGCATTGCGTGAAGATCCGGATATCATCCTGGTTGGCGAGTTGCGCGATCTTGAAACCATCCGCTTGG
CACTCACCGCTGCGGAAACCGGCCATCTGGTGTTCGGTACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGATGTCTTCCCTGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCCTTGCGCGCGGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGTGGACGCACCGCCGCCTGGGAAATCATGGTTGGCACCCCGGCCATCCGTAACCTGATCC
GGGAGGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACCGGCCAGCAGTACGGCATGCAGACGCTGGATCAGCATTTA
CAGGACCTGATCAAACGCAGCCTGATCACGCGCAACCAAGCACGCGAATACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment