Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BEN51_RS05820 Genome accession   NZ_CP016786
Coordinates   1222974..1224029 (-) Length   351 a.a.
NCBI ID   WP_119865138.1    Uniprot ID   A0A343JBU0
Organism   Clostridium isatidis strain DSM 15098     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1217974..1229029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN51_RS05795 (BEN51_05750) - 1218474..1218797 (-) 324 WP_119865133.1 YlmC/YmxH family sporulation protein -
  BEN51_RS05800 (BEN51_05755) sigG 1218845..1219618 (-) 774 WP_119865134.1 RNA polymerase sporulation sigma factor SigG -
  BEN51_RS05805 (BEN51_05760) sigE 1219699..1220406 (-) 708 WP_119865135.1 RNA polymerase sporulation sigma factor SigE -
  BEN51_RS05810 (BEN51_05765) - 1220415..1221212 (-) 798 WP_119865136.1 sigma-E processing peptidase SpoIIGA -
  BEN51_RS05815 (BEN51_05770) ftsZ 1221411..1222532 (-) 1122 WP_119865137.1 cell division protein FtsZ -
  BEN51_RS05820 (BEN51_05775) pilT 1222974..1224029 (-) 1056 WP_119865138.1 type IV pilus twitching motility protein PilT Machinery gene
  BEN51_RS05825 (BEN51_05780) sigK 1224135..1224830 (-) 696 WP_119865139.1 RNA polymerase sporulation sigma factor SigK -
  BEN51_RS05830 (BEN51_05785) - 1224989..1226614 (-) 1626 WP_119865140.1 penicillin-binding transpeptidase domain-containing protein -
  BEN51_RS05835 (BEN51_05790) udk 1226671..1227288 (-) 618 WP_119865141.1 uridine kinase -
  BEN51_RS05840 (BEN51_05795) - 1227314..1228549 (-) 1236 WP_119865142.1 peptidase U32 family protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38924.04 Da        Isoelectric Point: 8.3354

>NTDB_id=192228 BEN51_RS05820 WP_119865138.1 1222974..1224029(-) (pilT) [Clostridium isatidis strain DSM 15098]
MYALKDLLSKTIEIGASDLHLTVGLAPTVRKNGTLEKVGDHKLTPSELREFAKEILAEKYAEYNEVGEIDTSYSISGIGR
FRVNIFKQRNSDAIAIRVIALKIPTLDELKHPKVIKEILKKQRGLVLVTGPTGSGKSTTLAAIINEINNTREAHIITLED
PIEYLHKHNKSIINQREIGKDSRTYESALKAILREDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPHQQQQIRIQLAAVLQGVISQQLVPTQKNNGRVAALEIMVTTPGIQNLIREGKTYQIESAIQTGGKYGMKTMDMA
LAELYKTGQIDLDTALSYSVDRETLKRIIMI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=192228 BEN51_RS05820 WP_119865138.1 1222974..1224029(-) (pilT) [Clostridium isatidis strain DSM 15098]
ATGTATGCATTAAAAGATCTATTAAGCAAAACTATAGAGATAGGAGCATCAGACTTACATTTAACAGTTGGATTAGCTCC
AACTGTAAGAAAAAATGGTACTTTAGAGAAAGTAGGTGATCATAAATTAACTCCTAGTGAATTAAGAGAATTTGCAAAAG
AAATATTAGCAGAAAAGTATGCTGAATACAATGAAGTGGGAGAAATCGATACTTCCTACTCAATTTCTGGAATTGGAAGA
TTTAGGGTAAATATATTTAAACAAAGAAATAGTGATGCTATTGCAATAAGAGTAATTGCATTAAAAATTCCTACTCTAGA
TGAATTAAAACATCCAAAAGTTATAAAAGAAATATTAAAAAAACAAAGGGGATTAGTTTTAGTAACAGGGCCAACTGGTA
GTGGTAAAAGTACTACCCTTGCGGCTATAATAAATGAAATAAATAATACAAGAGAAGCACATATTATAACGTTAGAGGAT
CCTATCGAATATCTTCATAAGCATAATAAATCAATTATTAATCAAAGAGAAATAGGAAAAGATAGTAGAACTTATGAAAG
TGCATTAAAAGCTATTTTAAGAGAAGATCCTGATGTTATCCTTGTAGGGGAGATGAGAGACCTGGAAACAATATCTATTG
CCTTAACAGCGGCAGAAACAGGACACTTAGTTTTCTCTACATTACATACAATAGGGGCAGCTAAGACAATAGATAGAATA
ATAGATGTTTTTCCGCCGCACCAACAGCAACAAATTAGAATCCAGCTTGCAGCAGTTTTACAAGGAGTAATATCGCAACA
ATTAGTTCCTACTCAAAAGAATAATGGAAGAGTTGCTGCTTTAGAAATTATGGTCACAACTCCAGGTATTCAAAATTTAA
TAAGAGAAGGAAAAACATATCAAATAGAATCAGCTATTCAAACTGGTGGAAAATATGGTATGAAAACAATGGATATGGCT
CTAGCTGAACTATATAAAACAGGACAAATAGATTTAGATACTGCTTTAAGTTATTCTGTAGATAGGGAAACTTTAAAAAG
AATTATAATGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A343JBU0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

49.271

97.721

0.481
  pilT Pseudomonas aeruginosa PAK

49.85

94.872

0.473
  pilT Vibrio cholerae strain A1552

50.152

93.732

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

93.732

0.47
  pilT Legionella pneumophila strain ERS1305867

49.107

95.726

0.47
  pilT Legionella pneumophila strain Lp02

49.107

95.726

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.399

98.575

0.467
  pilT Acinetobacter nosocomialis M2

47.813

97.721

0.467
  pilT Acinetobacter baumannii D1279779

47.813

97.721

0.467
  pilT Acinetobacter baumannii strain A118

47.813

97.721

0.467
  pilT Pseudomonas stutzeri DSM 10701

48.949

94.872

0.464
  pilT Neisseria meningitidis 8013

47.23

97.721

0.462
  pilT Neisseria gonorrhoeae MS11

47.23

97.721

0.462
  pilU Pseudomonas stutzeri DSM 10701

40.922

98.86

0.405
  pilU Acinetobacter baylyi ADP1

41.124

96.296

0.396
  pilU Vibrio cholerae strain A1552

39.286

95.726

0.376

Multiple sequence alignment