Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I858_RS06905 Genome accession   NZ_CP016540
Coordinates   1364653..1365693 (+) Length   346 a.a.
NCBI ID   WP_065524224.1    Uniprot ID   A0A1B1RXI6
Organism   Planococcus versutus strain L10.15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1359653..1370693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I858_RS06880 (I858_000985) - 1359830..1360375 (-) 546 WP_065524228.1 PulJ/GspJ family protein -
  I858_RS06885 (I858_000980) - 1360368..1360784 (-) 417 WP_065524886.1 type IV pilus modification PilV family protein -
  I858_RS06890 (I858_000975) - 1361099..1361497 (+) 399 WP_065524227.1 hypothetical protein -
  I858_RS06895 (I858_000970) - 1361490..1362968 (+) 1479 WP_065524226.1 VanW family protein -
  I858_RS06900 (I858_000965) - 1362990..1364648 (+) 1659 WP_065524225.1 GspE/PulE family protein -
  I858_RS06905 (I858_000960) pilT 1364653..1365693 (+) 1041 WP_065524224.1 type IV pilus twitching motility protein PilT Machinery gene
  I858_RS06910 (I858_000955) - 1365695..1366906 (+) 1212 WP_065524223.1 type II secretion system F family protein -
  I858_RS06915 (I858_000950) - 1367038..1367511 (+) 474 WP_065524222.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I858_RS06920 (I858_000945) pilM 1367558..1368484 (+) 927 WP_065524221.1 type IV pilus biogenesis protein PilM -
  I858_RS06925 (I858_000940) - 1368487..1369206 (+) 720 WP_065524220.1 fimbrial assembly protein -
  I858_RS06930 (I858_000935) - 1369203..1369997 (+) 795 WP_065524219.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38079.63 Da        Isoelectric Point: 7.0244

>NTDB_id=189027 I858_RS06905 WP_065524224.1 1364653..1365693(+) (pilT) [Planococcus versutus strain L10.15]
MSIPYIDEILTAAINLDASDIHLTVGIPPVFRLNGKLKRYGEEIVTYEQTREISKLLIPERLFERFLEKGEMDYSYSLPG
VGRFRVNTFHQRGSDSHAFRTITTGVPTIDQLNMPDVLKILSESQQGLILVTGPTGSGKSTTLAAMIRHINDHMAKHIIT
LEDPIEYLHRHGSSIINQREIGSDTHSFANGLRAALRQDPDVILVGEMRDLETITTAITAAETGHLVMATLHTSSAASTI
ERIIDVFPAGQQPQVRTQLAGVLKAVISQRLLPTSDGQGRVAATEIMINNTAISNLIRTEKVHQIPNVILTNRAAGMNMM
ATSIQELLATNKISRHTAHPYLKGEG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=189027 I858_RS06905 WP_065524224.1 1364653..1365693(+) (pilT) [Planococcus versutus strain L10.15]
ATGTCAATTCCATATATCGATGAAATTTTAACCGCTGCCATTAATTTGGATGCGTCCGATATCCATTTAACAGTAGGAAT
TCCACCCGTTTTTCGATTGAATGGCAAACTAAAGCGGTACGGTGAAGAAATTGTGACTTACGAGCAAACGAGAGAAATTA
GCAAGTTGTTGATTCCGGAGAGGTTGTTTGAACGCTTTTTAGAAAAAGGAGAAATGGATTACTCTTATTCGCTACCCGGT
GTAGGCAGGTTTCGTGTCAATACATTTCATCAGCGAGGATCGGATTCCCATGCGTTTCGAACGATCACTACGGGAGTGCC
GACAATTGATCAGTTGAACATGCCAGATGTTTTGAAAATTCTTTCTGAATCACAACAAGGTCTCATTTTAGTCACAGGGC
CTACTGGGTCTGGCAAATCAACAACTTTAGCCGCTATGATTCGCCATATAAACGATCATATGGCAAAACACATCATCACT
TTAGAAGATCCGATTGAGTACTTGCATAGGCATGGCAGTTCGATTATAAACCAACGTGAAATCGGGTCGGATACCCATTC
TTTTGCGAACGGCTTACGCGCAGCTTTGCGGCAAGATCCGGATGTTATTTTAGTCGGTGAAATGCGTGACTTAGAAACCA
TTACGACAGCTATCACAGCTGCCGAAACTGGCCATTTGGTTATGGCCACTTTGCACACATCAAGTGCTGCATCAACAATT
GAACGAATTATTGATGTGTTTCCAGCAGGTCAGCAGCCTCAAGTTCGTACACAATTAGCAGGTGTCTTAAAAGCAGTCAT
TTCTCAGCGGTTATTGCCAACAAGTGACGGGCAAGGACGAGTGGCCGCAACGGAAATTATGATCAACAATACAGCTATTT
CCAATTTAATCCGTACAGAAAAAGTGCATCAAATTCCAAACGTTATTTTAACCAATCGTGCAGCGGGGATGAATATGATG
GCCACTTCTATACAAGAGCTATTAGCAACGAATAAAATTTCTCGCCATACCGCTCATCCATACTTGAAAGGAGAAGGGTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B1RXI6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.632

93.353

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.235

93.642

0.48
  pilT Vibrio cholerae strain A1552

51.235

93.642

0.48
  pilT Acinetobacter baylyi ADP1

48.521

97.688

0.474
  pilT Acinetobacter baumannii strain A118

47.929

97.688

0.468
  pilT Pseudomonas stutzeri DSM 10701

47.929

97.688

0.468
  pilT Acinetobacter baumannii D1279779

47.929

97.688

0.468
  pilT Pseudomonas aeruginosa PAK

47.633

97.688

0.465
  pilT Acinetobacter nosocomialis M2

47.633

97.688

0.465
  pilT Neisseria meningitidis 8013

49.536

93.353

0.462
  pilT Neisseria gonorrhoeae MS11

49.226

93.353

0.46
  pilT Legionella pneumophila strain Lp02

46.407

96.532

0.448
  pilT Legionella pneumophila strain ERS1305867

46.407

96.532

0.448
  pilU Pseudomonas stutzeri DSM 10701

41.141

96.243

0.396
  pilU Vibrio cholerae strain A1552

39.82

96.532

0.384
  pilU Acinetobacter baylyi ADP1

39.394

95.376

0.376

Multiple sequence alignment