Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BBI15_RS07225 Genome accession   NZ_CP016539
Coordinates   1424961..1426013 (+) Length   350 a.a.
NCBI ID   WP_068868947.1    Uniprot ID   A0A1C7E655
Organism   Planococcus plakortidis strain DSM 23997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1419961..1431013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBI15_RS07210 (BBI15_02665) - 1421433..1421843 (+) 411 WP_068868945.1 hypothetical protein -
  BBI15_RS07215 (BBI15_02670) - 1421845..1423278 (+) 1434 WP_068868946.1 VanW family protein -
  BBI15_RS07220 (BBI15_02675) - 1423301..1424959 (+) 1659 WP_208599452.1 GspE/PulE family protein -
  BBI15_RS07225 (BBI15_02680) pilT 1424961..1426013 (+) 1053 WP_068868947.1 type IV pilus twitching motility protein PilT Machinery gene
  BBI15_RS07230 (BBI15_02685) - 1426014..1427225 (+) 1212 WP_068868948.1 type II secretion system F family protein -
  BBI15_RS16755 (BBI15_02690) - 1427360..1427788 (+) 429 WP_068868949.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BBI15_RS07240 (BBI15_02695) pilM 1427814..1428749 (+) 936 WP_068868950.1 type IV pilus biogenesis protein PilM -
  BBI15_RS07245 (BBI15_02700) - 1428752..1429486 (+) 735 WP_068868951.1 PilN domain-containing protein -
  BBI15_RS07250 (BBI15_02705) - 1429483..1430238 (+) 756 WP_068868952.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38470.29 Da        Isoelectric Point: 6.9515

>NTDB_id=188987 BBI15_RS07225 WP_068868947.1 1424961..1426013(+) (pilT) [Planococcus plakortidis strain DSM 23997]
MTETAIDFIDKVLTAARELGVSDIHMTTGIPPVFRMHGSLKRFGEERLLPEDTESIAKALMPESLWDTFLQKGEMDYSYS
IPGVARFRVNSFHQRGSISHAFRTIPTVIPSIDDLKMPDTLKKLADTHQGLILVTGPTGSGKSTTLAAMIRHMNEHLTKH
IITLEDPIEYMHKHGSSVIDQREVGFDTKSFANGLRAALRQDPDVILVGEMRDLETITTAITAAETGHLVMGTLHTSSAA
STVERIIDVFPPEQHAQIRTQLGGILKAVISQRLLPTADGKGRVAATEIMIHNTAIANLIRTEKVHQIPNVILTNRAAGM
HLMQTSVQDLLNKGRITKQIAAPYLIGGEE

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=188987 BBI15_RS07225 WP_068868947.1 1424961..1426013(+) (pilT) [Planococcus plakortidis strain DSM 23997]
ATGACAGAAACCGCTATTGATTTTATCGATAAAGTATTGACCGCGGCGCGTGAACTGGGCGTCTCCGATATCCATATGAC
GACCGGCATCCCGCCGGTATTCCGCATGCACGGATCGCTCAAGCGCTTCGGGGAGGAGCGACTGTTGCCGGAAGATACCG
AATCAATCGCGAAAGCCTTGATGCCGGAAAGCCTGTGGGACACGTTCCTGCAAAAAGGCGAGATGGATTATTCCTACTCG
ATTCCTGGCGTCGCCCGTTTCCGCGTCAACTCATTCCACCAGCGCGGATCCATCTCCCATGCGTTCCGGACCATCCCGAC
GGTCATCCCATCGATCGATGATCTGAAAATGCCGGATACTTTAAAGAAACTGGCCGACACGCACCAAGGCCTGATCCTCG
TCACCGGCCCGACCGGTTCCGGGAAATCGACGACTTTGGCGGCGATGATCCGCCATATGAATGAACATTTGACGAAGCAC
ATCATCACGCTCGAAGACCCGATCGAGTATATGCATAAGCACGGTTCATCGGTCATCGACCAGCGCGAAGTCGGATTCGA
CACGAAATCATTCGCCAACGGCTTGCGCGCAGCACTGCGCCAGGATCCGGACGTCATTTTGGTCGGTGAGATGCGTGACC
TCGAGACGATCACGACCGCAATCACCGCAGCGGAAACCGGCCACTTGGTCATGGGGACGCTCCACACCTCGAGCGCCGCC
TCGACCGTTGAACGGATCATCGACGTCTTTCCACCGGAACAGCACGCCCAGATCCGCACGCAGCTCGGCGGCATCCTGAA
AGCGGTCATCTCGCAGCGCCTGCTTCCGACGGCGGACGGCAAAGGGCGCGTTGCCGCGACCGAGATCATGATCCACAACA
CGGCGATCGCGAACTTGATCCGCACCGAGAAAGTCCACCAGATCCCGAACGTCATCCTGACGAACCGGGCGGCGGGCATG
CATCTCATGCAGACCTCCGTCCAGGACTTGCTCAATAAAGGACGCATCACGAAACAGATCGCGGCGCCTTATTTGATCGG
AGGCGAAGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C7E655

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.898

95.429

0.486
  pilT Neisseria meningitidis 8013

50.625

91.429

0.463
  pilT Neisseria gonorrhoeae MS11

50.313

91.429

0.46
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.629

90.857

0.46
  pilT Vibrio cholerae strain A1552

50.629

90.857

0.46
  pilT Acinetobacter baumannii D1279779

48.048

95.143

0.457
  pilT Acinetobacter nosocomialis M2

48.048

95.143

0.457
  pilT Acinetobacter baumannii strain A118

48.048

95.143

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.447

95.143

0.451
  pilT Pseudomonas aeruginosa PAK

46.847

95.143

0.446
  pilT Acinetobacter baylyi ADP1

49.367

90.286

0.446
  pilT Legionella pneumophila strain ERS1305867

48.438

91.429

0.443
  pilT Legionella pneumophila strain Lp02

48.438

91.429

0.443
  pilU Pseudomonas stutzeri DSM 10701

41.742

95.143

0.397
  pilU Vibrio cholerae strain A1552

39.82

95.429

0.38
  pilU Acinetobacter baylyi ADP1

39.339

95.143

0.374

Multiple sequence alignment