Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BBI11_RS06970 Genome accession   NZ_CP016538
Coordinates   1389504..1390544 (+) Length   346 a.a.
NCBI ID   WP_068461821.1    Uniprot ID   -
Organism   Planococcus maritimus strain DSM 17275     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1384504..1395544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBI11_RS06945 (BBI11_07805) - 1384654..1385205 (-) 552 WP_068461814.1 PulJ/GspJ family protein -
  BBI11_RS06950 (BBI11_07810) - 1385189..1385596 (-) 408 WP_068461816.1 type IV pilus modification PilV family protein -
  BBI11_RS06955 (BBI11_07815) - 1386023..1386382 (+) 360 WP_068461817.1 hypothetical protein -
  BBI11_RS06960 (BBI11_07820) - 1386384..1387817 (+) 1434 WP_068461820.1 VanW family protein -
  BBI11_RS06965 (BBI11_07825) - 1387838..1389499 (+) 1662 WP_208597182.1 GspE/PulE family protein -
  BBI11_RS06970 (BBI11_07830) pilT 1389504..1390544 (+) 1041 WP_068461821.1 type IV pilus twitching motility protein PilT Machinery gene
  BBI11_RS06975 (BBI11_07835) - 1390545..1391756 (+) 1212 WP_068461823.1 type II secretion system F family protein -
  BBI11_RS06980 (BBI11_07840) - 1391886..1392335 (+) 450 WP_068461826.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BBI11_RS06985 (BBI11_07845) pilM 1392361..1393299 (+) 939 WP_068461828.1 type IV pilus biogenesis protein PilM -
  BBI11_RS06990 (BBI11_07850) - 1393300..1394040 (+) 741 WP_237150329.1 fimbrial assembly protein -
  BBI11_RS06995 (BBI11_07855) - 1394037..1394792 (+) 756 WP_068461831.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37925.52 Da        Isoelectric Point: 6.8325

>NTDB_id=188949 BBI11_RS06970 WP_068461821.1 1389504..1390544(+) (pilT) [Planococcus maritimus strain DSM 17275]
MPTTYIDKVLTQARNLNVSDIHLTTGIPPVFRMHGTLKRYGDENLTPDMTKEIAKALMPELLWQQFLDKGEMDFSYSLSG
VARFRVNSFHQRGSISHAFRTIPTEIPTIADLNMPKILANLAETQQGLILVTGPTGSGKSTTLAAMIRHINENLTKHIIT
LEDPIEYLHRHGSSIIDQREVGFDTDSFAAGLRAALRQDPDVVLVGEMRDLETISTAITAAETGHLVMATLHTSSAASTI
ERIIDVFPHGQQAQVRTQLAGILKAVVSQRLLPTADGTGRIAATEILINNPAVANLIRTEKVHQIPNVILTNRSFGMHMM
VSSVQELLATGKISRQTAQPFLEGGE

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=188949 BBI11_RS06970 WP_068461821.1 1389504..1390544(+) (pilT) [Planococcus maritimus strain DSM 17275]
ATGCCAACCACTTATATAGATAAAGTCCTGACGCAAGCACGCAACTTAAATGTTTCGGATATTCATTTGACGACCGGCAT
TCCGCCCGTTTTCCGCATGCATGGCACCTTAAAGCGCTACGGTGATGAAAACTTGACGCCCGACATGACCAAGGAAATCG
CCAAAGCGTTAATGCCGGAACTTCTTTGGCAGCAATTTCTAGATAAAGGCGAGATGGATTTTTCCTATTCGCTTTCTGGA
GTTGCCCGTTTCCGAGTCAATTCCTTCCACCAGCGCGGCTCGATTTCGCATGCGTTCCGAACTATTCCAACCGAAATACC
GACAATCGCTGATTTGAATATGCCGAAAATCTTGGCGAATTTGGCAGAAACCCAGCAAGGACTTATTTTAGTGACAGGGC
CGACTGGGTCAGGAAAGTCGACCACCTTGGCAGCAATGATTCGCCACATTAATGAAAATTTAACTAAACACATCATCACA
TTAGAAGACCCAATTGAGTATTTGCACCGCCATGGTTCATCTATTATTGACCAGCGCGAAGTTGGTTTTGACACGGATTC
ATTTGCAGCCGGTTTGCGTGCAGCTTTGCGCCAAGATCCAGATGTCGTTTTGGTAGGTGAAATGCGTGATCTGGAAACAA
TTTCGACCGCCATCACCGCCGCGGAAACCGGTCACCTTGTGATGGCTACCCTGCATACGTCCAGTGCAGCTTCGACAATC
GAGCGAATCATCGATGTGTTTCCGCACGGACAGCAAGCGCAAGTGCGCACGCAACTCGCCGGAATTTTAAAAGCGGTCGT
GTCCCAGCGTTTGCTGCCGACCGCTGACGGCACAGGACGAATCGCTGCAACAGAAATTCTCATTAACAACCCTGCGGTGG
CGAACTTAATTCGCACTGAAAAAGTTCATCAAATTCCGAATGTTATTTTGACGAACCGTTCATTCGGAATGCATATGATG
GTGTCGTCTGTTCAAGAATTATTGGCGACTGGCAAAATCAGCCGCCAGACCGCCCAACCGTTCCTTGAAGGGGGTGAGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.651

91.04

0.488
  pilT Pseudomonas stutzeri DSM 10701

47.801

98.555

0.471
  pilT Vibrio cholerae strain A1552

49.541

94.509

0.468
  pilT Neisseria meningitidis 8013

49.541

94.509

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

94.509

0.468
  pilT Neisseria gonorrhoeae MS11

49.235

94.509

0.465
  pilT Pseudomonas aeruginosa PAK

46.628

98.555

0.46
  pilT Acinetobacter baumannii D1279779

49.221

92.775

0.457
  pilT Acinetobacter baumannii strain A118

49.221

92.775

0.457
  pilT Acinetobacter nosocomialis M2

48.91

92.775

0.454
  pilT Acinetobacter baylyi ADP1

48.91

92.775

0.454
  pilT Legionella pneumophila strain ERS1305867

47.84

93.642

0.448
  pilT Legionella pneumophila strain Lp02

47.84

93.642

0.448
  pilU Pseudomonas stutzeri DSM 10701

44.062

92.486

0.408
  pilU Vibrio cholerae strain A1552

40.419

96.532

0.39
  pilU Acinetobacter baylyi ADP1

40.81

92.775

0.379

Multiple sequence alignment