Detailed information NTG0188913

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: BBI08_RS06995
• Genome accession: NZ_CP016537
• Coordinates: 1396862..1397905 (+)
• Length: 347 a.a.
• NCBI ID: WP_008498913.1
• Uniprot ID: -
• Organism: Planococcus halocryophilus strain DSM 24743
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1393735..1402227	1396862..1397905	within	0

Gene organization within MGE regions

Location: 1393735..1402227

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BBI08_RS06985 (BBI08_02655)	-	1393735..1395177 (+)	1443	WP_065527899.1	VanW family protein	-
BBI08_RS06990 (BBI08_02660)	-	1395200..1396858 (+)	1659	WP_008498912.1	GspE/PulE family protein	-
BBI08_RS06995 (BBI08_02665)	pilT	1396862..1397905 (+)	1044	WP_008498913.1	type IV pilus twitching motility protein PilT	Machinery gene
BBI08_RS07000 (BBI08_02670)	-	1397907..1399118 (+)	1212	WP_008498914.1	type II secretion system F family protein	-
BBI08_RS07005 (BBI08_02675)	-	1399250..1399699 (+)	450	WP_008498915.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
BBI08_RS07010 (BBI08_02680)	pilM	1399751..1400689 (+)	939	WP_008498916.1	type IV pilus biogenesis protein PilM	-
BBI08_RS07015 (BBI08_02685)	-	1400858..1401469 (+)	612	WP_237146580.1	fimbrial assembly protein	-
BBI08_RS07020 (BBI08_02690)	-	1401466..1402227 (+)	762	WP_065527900.1	pilus assembly protein PilO	-

Sequence

Protein

• Length: 347 a.a.
• Molecular weight: 38441.28 Da
• Isoelectric Point: 7.0599

>NTDB_id=188913 BBI08_RS06995 WP_008498913.1 1396862..1397905(+) (pilT) [Planococcus halocryophilus strain DSM 24743]
MPTTTFIDHILTEAKERNVSDIHMTTGIPPIFRMNGTLIQFGDKKLMPDDTMAIAKALMPESLWDTFLEKGEMDYSYSIP
GVARYRVNSFHQRGSISHAFRTIPSRIPSIDDLQMPDTLKKLADTHQGLILVTGPTGSGKSTTLAAMIRYMNEHMTKHII
TLEDPIEYMHRHGTSVIDQREVGFDTNSFANGLRAALRQDPDVILVGEMRDLETITTAITAAETGHLVMGTLHTSSAAST
IERIIDVFPHEQHAQIRTQLGGIIKAVISQRLLPTADGKGRVAATEIMISNPAIANLIRSEKVHQIPNVILTNRASGMHM
METSVQELLKKGKITREIAQPYLKGAE

Nucleotide

• Length: 1044 bp

>NTDB_id=188913 BBI08_RS06995 WP_008498913.1 1396862..1397905(+) (pilT) [Planococcus halocryophilus strain DSM 24743]
ATGCCTACTACAACATTTATCGATCACATATTGACTGAAGCCAAAGAACGCAATGTCTCCGATATCCACATGACCACCGG
CATCCCGCCTATTTTCCGGATGAACGGCACCTTGATCCAGTTTGGCGACAAAAAGCTGATGCCGGACGACACGATGGCCA
TCGCCAAAGCGTTGATGCCGGAATCCTTGTGGGACACCTTCCTGGAAAAAGGCGAGATGGATTATTCCTACTCGATTCCC
GGCGTAGCACGTTACCGCGTCAACTCATTCCATCAGCGCGGTTCGATTTCGCACGCCTTCCGGACGATCCCTTCTCGAAT
CCCGTCAATCGACGACCTGCAGATGCCGGATACCTTGAAAAAGCTTGCCGATACGCATCAAGGGCTGATTCTCGTGACGG
GTCCGACCGGTTCCGGTAAGTCGACAACGCTCGCCGCCATGATCCGTTATATGAATGAACACATGACCAAACACATCATC
ACCTTGGAAGATCCGATCGAGTACATGCACCGCCATGGCACATCGGTCATCGACCAGCGCGAAGTCGGCTTTGATACCAA
CTCGTTTGCCAATGGCTTGCGTGCGGCATTGCGGCAGGATCCCGATGTCATCCTGGTAGGTGAGATGCGGGATCTGGAAA
CGATCACTACCGCCATCACCGCTGCTGAAACCGGCCACTTGGTCATGGGCACGCTGCATACTTCGAGCGCCGCTTCTACC
ATCGAGCGGATTATAGACGTTTTCCCGCATGAACAACATGCCCAGATTCGGACGCAGCTCGGCGGTATCATCAAAGCCGT
CATTTCCCAGCGTTTGTTGCCGACGGCTGACGGCAAAGGGCGGGTTGCTGCAACCGAAATCATGATTTCCAATCCAGCCA
TCGCCAACCTGATCCGCTCCGAGAAAGTCCACCAGATCCCGAACGTCATCCTGACCAACCGGGCGTCCGGCATGCACATG
ATGGAAACGTCGGTTCAGGAGCTGCTGAAAAAAGGCAAGATCACACGTGAAATCGCCCAGCCATACTTGAAAGGAGCGGA
ATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	49.853	98.271	0.49
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.817	97.406	0.476
pilT	Vibrio cholerae strain A1552	48.817	97.406	0.476
pilT	Pseudomonas stutzeri DSM 10701	47.801	98.271	0.47
pilT	Acinetobacter baylyi ADP1	47.801	98.271	0.47
pilT	Acinetobacter baumannii D1279779	47.507	98.271	0.467
pilT	Neisseria meningitidis 8013	47.507	98.271	0.467
pilT	Acinetobacter nosocomialis M2	47.507	98.271	0.467
pilT	Acinetobacter baumannii strain A118	47.507	98.271	0.467
pilT	Neisseria gonorrhoeae MS11	47.214	98.271	0.464
pilT	Pseudomonas aeruginosa PAK	47.633	97.406	0.464
pilT	Legionella pneumophila strain ERS1305867	47.447	95.965	0.455
pilT	Legionella pneumophila strain Lp02	47.447	95.965	0.455
pilU	Pseudomonas stutzeri DSM 10701	41.975	93.372	0.392
pilU	Vibrio cholerae strain A1552	38.938	97.695	0.38
pilU	Acinetobacter baylyi ADP1	40.063	91.354	0.366