Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BBH88_RS07540 Genome accession   NZ_CP016534
Coordinates   1510490..1511533 (+) Length   347 a.a.
NCBI ID   WP_065537033.1    Uniprot ID   -
Organism   Planococcus antarcticus DSM 14505     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1505490..1516533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBH88_RS07525 (BBH88_13950) - 1506810..1507217 (+) 408 WP_065537035.1 hypothetical protein -
  BBH88_RS07530 (BBH88_13945) - 1507210..1508805 (+) 1596 WP_065537034.1 VanW family protein -
  BBH88_RS07535 (BBH88_13940) - 1508828..1510486 (+) 1659 WP_197684184.1 GspE/PulE family protein -
  BBH88_RS07540 (BBH88_13935) pilT 1510490..1511533 (+) 1044 WP_065537033.1 type IV pilus twitching motility protein PilT Machinery gene
  BBH88_RS07545 (BBH88_13930) - 1511535..1512746 (+) 1212 WP_065537032.1 type II secretion system F family protein -
  BBH88_RS07550 (BBH88_13925) - 1512879..1513340 (+) 462 WP_065537031.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BBH88_RS07555 (BBH88_13920) pilM 1513376..1514314 (+) 939 WP_065537030.1 type IV pilus biogenesis protein PilM -
  BBH88_RS07560 (BBH88_13915) - 1514321..1515097 (+) 777 WP_065537029.1 PilN domain-containing protein -
  BBH88_RS07565 (BBH88_13910) - 1515094..1515849 (+) 756 WP_065537028.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38359.21 Da        Isoelectric Point: 7.0599

>NTDB_id=188875 BBH88_RS07540 WP_065537033.1 1510490..1511533(+) (pilT) [Planococcus antarcticus DSM 14505]
MPTTTFIDHVLTEAKERNVSDIHMTTGIPPIFRMNGTLIQFGDKKLMPEDTMAIAKALMPESLWDTFLEKGEMDYSYSIA
GVARYRVNSFHQRGSISHAFRTIPSKIPSIDDLQMPDTLKKLADTHQGLILVTGPTGSGKSTTLAAMIRYMNEHMTKHII
TLEDPIEYMHKHGTSVIDQREVGFDTNSFANGLRAALRQDPDVILVGEMRDLETITTAITAAETGHLVMGTLHTSSAAST
IERIIDVFPHEQHAQIRTQLGGIIKAVISQRLLPTADGKGRVAATEIMISNPAIANLIRSEKVHQIPNVILTNRASGMHM
METSVQELLKKGKITREIAQPYLKGAE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=188875 BBH88_RS07540 WP_065537033.1 1510490..1511533(+) (pilT) [Planococcus antarcticus DSM 14505]
ATGCCTACTACAACTTTCATCGATCATGTATTGACCGAAGCCAAAGAACGCAATGTCTCCGATATCCACATGACCACCGG
TATCCCGCCAATTTTCCGGATGAATGGCACCTTGATCCAGTTCGGCGACAAAAAACTGATGCCAGAAGACACCATGGCCA
TCGCCAAAGCGTTGATGCCGGAATCGCTATGGGACACGTTCCTTGAAAAAGGCGAGATGGATTATTCCTACTCAATCGCA
GGCGTGGCGCGTTACCGGGTCAACTCATTCCACCAGCGCGGCTCGATTTCGCACGCCTTCCGGACGATCCCTTCCAAAAT
CCCGTCAATCGACGACCTGCAGATGCCAGATACTTTGAAAAAGCTGGCCGATACCCATCAAGGGCTGATCCTCGTGACCG
GTCCAACCGGCTCTGGTAAGTCGACAACACTTGCCGCCATGATCCGCTATATGAATGAACACATGACCAAACACATTATC
ACACTCGAAGATCCGATCGAGTATATGCATAAGCATGGCACCTCGGTCATCGACCAGCGCGAAGTCGGCTTTGATACCAA
TTCGTTCGCCAACGGCCTGCGCGCCGCACTCAGGCAAGATCCCGATGTCATTCTGGTCGGAGAGATGCGGGATCTGGAAA
CGATCACCACCGCTATCACTGCCGCTGAAACCGGTCACTTGGTTATGGGCACCTTGCATACTTCGAGTGCCGCATCGACC
ATCGAGCGGATCATCGACGTGTTCCCGCACGAACAGCACGCCCAGATCCGGACACAGCTTGGCGGTATTATCAAAGCGGT
CATTTCCCAGCGCTTGCTACCTACCGCTGACGGCAAGGGGCGTGTGGCCGCAACCGAGATCATGATCTCCAATCCGGCCA
TCGCCAACTTGATCCGTTCCGAAAAAGTCCACCAAATCCCAAACGTTATTTTGACCAACCGGGCGTCAGGCATGCATATG
ATGGAAACCTCTGTACAAGAACTGCTGAAAAAAGGCAAGATCACGCGTGAAATCGCGCAGCCTTACTTGAAAGGAGCGGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.56

98.271

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.521

97.406

0.473
  pilT Vibrio cholerae strain A1552

48.521

97.406

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.801

98.271

0.47
  pilT Acinetobacter baumannii D1279779

47.507

98.271

0.467
  pilT Neisseria meningitidis 8013

47.507

98.271

0.467
  pilT Acinetobacter nosocomialis M2

47.507

98.271

0.467
  pilT Acinetobacter baumannii strain A118

47.507

98.271

0.467
  pilT Acinetobacter baylyi ADP1

47.507

98.271

0.467
  pilT Neisseria gonorrhoeae MS11

47.214

98.271

0.464
  pilT Pseudomonas aeruginosa PAK

47.633

97.406

0.464
  pilT Legionella pneumophila strain Lp02

47.748

95.965

0.458
  pilT Legionella pneumophila strain ERS1305867

47.748

95.965

0.458
  pilU Pseudomonas stutzeri DSM 10701

42.284

93.372

0.395
  pilU Vibrio cholerae strain A1552

38.643

97.695

0.378
  pilU Acinetobacter baylyi ADP1

40.063

91.354

0.366

Multiple sequence alignment