Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BBF96_RS14750 Genome accession   NZ_CP016379
Coordinates   3252469..3253551 (+) Length   360 a.a.
NCBI ID   WP_127017857.1    Uniprot ID   A0A3Q9HSE4
Organism   Anoxybacter fermentans strain DY22613     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3247469..3258551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBF96_RS14735 (BBF96_14535) - 3247861..3249843 (+) 1983 WP_127017854.1 methyl-accepting chemotaxis protein -
  BBF96_RS14740 (BBF96_14540) - 3249915..3250244 (+) 330 WP_257792030.1 L,D-transpeptidase -
  BBF96_RS14745 (BBF96_14545) gspE 3250782..3252452 (+) 1671 WP_127017856.1 type II secretion system ATPase GspE -
  BBF96_RS14750 (BBF96_14550) pilT 3252469..3253551 (+) 1083 WP_127017857.1 type IV pilus twitching motility protein PilT Machinery gene
  BBF96_RS14755 (BBF96_14555) - 3253777..3254985 (+) 1209 WP_127017858.1 type II secretion system F family protein -
  BBF96_RS14760 (BBF96_14560) - 3255084..3255461 (+) 378 WP_127018297.1 type II secretion system protein -
  BBF96_RS14765 (BBF96_14565) - 3255531..3255941 (+) 411 WP_127017859.1 hypothetical protein -
  BBF96_RS14770 (BBF96_14570) - 3255962..3256684 (+) 723 WP_127017860.1 type II secretion system protein -
  BBF96_RS14775 (BBF96_14575) - 3256705..3257790 (+) 1086 WP_127017861.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40412.57 Da        Isoelectric Point: 6.4999

>NTDB_id=187621 BBF96_RS14750 WP_127017857.1 3252469..3253551(+) (pilT) [Anoxybacter fermentans strain DY22613]
MINVDQLLCDVAFDPLISDLHLTVNSPPIVRINGDLQPLARYESVLSYKQLIEIAKYLMSEEQYREFEEKGEYDFSYSLE
SVGRFRVNAYFQRGTVSLALRVIPNKIPSIDDLGLPLVIKELAMKKNGLILCTGPTGSGKSTTLASMLDLINCTRTCHII
TLEDPIEYLHTHKKSLIHQREIGADTQSFASGLRAALRQDPDVILVGEMRDLETISIALEAAETGHLVLATLHTNNAPGA
IDRIIDVFPPNQQQQIRIQLATVIQGVIAQQLVKRVDRRGRVAAVEVMVATPAIRNLIRENKAHQIYSVIQTGARFGMQT
MDNCLIRLYRDGIISFEDGRAKAVNVEYFERLARRPDQDF

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=187621 BBF96_RS14750 WP_127017857.1 3252469..3253551(+) (pilT) [Anoxybacter fermentans strain DY22613]
ATGATTAACGTTGATCAATTATTGTGTGATGTTGCTTTTGATCCATTGATTTCAGACCTGCATTTAACTGTAAATTCTCC
TCCTATAGTAAGAATCAACGGGGATTTACAACCTTTGGCCAGATATGAATCTGTTTTAAGTTATAAGCAATTGATTGAGA
TAGCTAAATATTTGATGAGCGAGGAGCAATATCGGGAGTTTGAAGAAAAAGGGGAATATGATTTCTCCTATAGTCTTGAG
TCAGTTGGTCGTTTTCGGGTTAATGCCTATTTTCAGCGGGGAACAGTAAGTTTAGCTTTAAGGGTTATTCCTAACAAGAT
TCCTTCCATTGATGATTTGGGTTTACCTCTGGTAATTAAGGAATTGGCGATGAAAAAAAATGGATTGATTTTATGTACAG
GCCCAACAGGAAGCGGTAAATCAACTACTTTGGCTTCAATGCTGGATTTAATCAATTGTACCCGGACATGTCATATTATC
ACCTTAGAAGATCCGATAGAATATCTTCATACTCATAAAAAAAGTTTAATTCATCAACGTGAAATTGGAGCAGATACTCA
GTCTTTTGCCAGCGGTTTACGGGCAGCCCTGCGTCAAGACCCGGATGTAATTTTGGTAGGAGAAATGCGTGATTTAGAAA
CTATTTCCATTGCGCTTGAAGCTGCAGAAACAGGTCATCTGGTTTTGGCAACTTTACATACTAATAATGCACCGGGAGCC
ATTGATCGAATTATCGACGTTTTTCCTCCAAATCAACAACAGCAGATTAGAATTCAGTTAGCAACGGTTATTCAGGGAGT
TATAGCCCAACAGTTGGTTAAACGCGTTGACAGGCGCGGGCGAGTGGCCGCGGTAGAAGTAATGGTGGCTACTCCTGCTA
TTCGTAATTTGATTCGTGAAAACAAAGCACATCAGATCTATTCGGTTATACAGACCGGGGCCAGGTTTGGAATGCAAACA
ATGGATAATTGCCTTATCCGGCTCTATCGGGATGGAATTATCAGTTTTGAGGATGGCCGGGCTAAAGCTGTTAATGTGGA
ATATTTTGAGCGACTGGCTCGCCGACCAGATCAGGATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9HSE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.061

95.278

0.506
  pilT Legionella pneumophila strain Lp02

50.904

92.222

0.469
  pilT Legionella pneumophila strain ERS1305867

50.904

92.222

0.469
  pilT Pseudomonas aeruginosa PAK

50.904

92.222

0.469
  pilT Acinetobacter baumannii strain A118

50.602

92.222

0.467
  pilT Acinetobacter nosocomialis M2

50.602

92.222

0.467
  pilT Acinetobacter baumannii D1279779

50.602

92.222

0.467
  pilT Pseudomonas stutzeri DSM 10701

50.301

92.222

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.077

90.278

0.461
  pilT Vibrio cholerae strain A1552

51.077

90.278

0.461
  pilT Neisseria meningitidis 8013

49.245

91.944

0.453
  pilT Acinetobacter baylyi ADP1

49.096

92.222

0.453
  pilT Neisseria gonorrhoeae MS11

48.485

91.667

0.444
  pilU Pseudomonas stutzeri DSM 10701

44.86

89.167

0.4
  pilU Acinetobacter baylyi ADP1

43.119

90.833

0.392
  pilU Vibrio cholerae strain A1552

41.615

89.444

0.372

Multiple sequence alignment