Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   A9P85_RS09660 Genome accession   NZ_CP016029
Coordinates   2165466..2165879 (-) Length   137 a.a.
NCBI ID   WP_042754743.1    Uniprot ID   -
Organism   Legionella pneumophila strain Pontiac     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2160466..2170879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9P85_RS09645 (A9P85_09645) letS 2160780..2163512 (+) 2733 WP_011946809.1 two-component system sensor histidine kinase LetS Regulator
  A9P85_RS09650 (A9P85_09650) - 2163610..2164854 (+) 1245 WP_011946810.1 6-phosphofructokinase -
  A9P85_RS09655 (A9P85_09655) pilA2 2164851..2165264 (-) 414 WP_011946811.1 pilin Machinery gene
  A9P85_RS09660 (A9P85_09660) pilA2 2165466..2165879 (-) 414 WP_042754743.1 pilin Machinery gene
  A9P85_RS09665 (A9P85_09665) - 2166182..2166499 (+) 318 WP_042754744.1 BolA family protein -
  A9P85_RS09670 (A9P85_09670) - 2166574..2167995 (+) 1422 WP_042754745.1 APC family permease -
  A9P85_RS09675 (A9P85_09675) - 2168126..2169556 (-) 1431 WP_042754746.1 cellulase family glycosylhydrolase -
  A9P85_RS09680 (A9P85_09680) kdsB 2169803..2170555 (-) 753 WP_027220383.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  A9P85_RS09685 (A9P85_09685) - 2170552..2170731 (-) 180 WP_010947637.1 Trm112 family protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14023.23 Da        Isoelectric Point: 8.4715

>NTDB_id=185495 A9P85_RS09660 WP_042754743.1 2165466..2165879(-) (pilA2) [Legionella pneumophila strain Pontiac]
MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVSEGLQMASSAKLAVSETAITNNALPPTQAATGYVSPAPTANV
TGIAIGANGVITITYTAAAGGGTIIMTPTLDANNGDVTWNCTGGSLLPKYRPASCRP

Nucleotide


Download         Length: 414 bp        

>NTDB_id=185495 A9P85_RS09660 WP_042754743.1 2165466..2165879(-) (pilA2) [Legionella pneumophila strain Pontiac]
ATGAGACAAAAGGGTTTTACATTAATTGAATTGATGATAGTCGTAGCGATTATCGGTATTTTGGCAGCAATCGCTATACC
AGCCTATCAAGATTACACCATACGAGCTCGTGTAAGCGAGGGGCTACAGATGGCAAGCTCAGCCAAACTTGCCGTATCTG
AAACGGCTATTACAAACAATGCGTTACCACCTACTCAGGCTGCAACGGGCTACGTGTCTCCTGCCCCCACGGCTAATGTG
ACAGGTATTGCAATAGGTGCTAACGGCGTGATTACCATCACGTATACCGCTGCAGCAGGTGGTGGTACGATTATTATGAC
TCCTACACTGGATGCCAACAACGGTGATGTTACCTGGAACTGCACCGGTGGTAGCTTATTGCCTAAATACCGGCCTGCCA
GTTGCAGACCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

89.781

100

0.898
  pilA2 Legionella pneumophila str. Paris

89.051

100

0.891
  pilA Ralstonia pseudosolanacearum GMI1000

47.826

100

0.562
  comP Acinetobacter baylyi ADP1

52.055

100

0.555
  pilE Neisseria gonorrhoeae MS11

39.241

100

0.453
  pilA Pseudomonas aeruginosa PAK

40.94

100

0.445
  pilA Haemophilus influenzae 86-028NP

41.096

100

0.438
  pilE Neisseria gonorrhoeae strain FA1090

37.58

100

0.431
  pilA/pilA1 Eikenella corrodens VA1

38.312

100

0.431
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.176

99.27

0.409
  pilA Glaesserella parasuis strain SC1401

40.441

99.27

0.401
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.849

100

0.394
  pilA Vibrio parahaemolyticus RIMD 2210633

40.8

91.241

0.372
  pilA Vibrio cholerae C6706

37.226

100

0.372
  pilA Vibrio cholerae strain A1552

37.226

100

0.372
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.226

100

0.372

Multiple sequence alignment