Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A9C19_RS05525 Genome accession   NZ_CP016020
Coordinates   1174734..1175774 (+) Length   346 a.a.
NCBI ID   WP_072579013.1    Uniprot ID   A0A1L3MPE3
Organism   Bacillus weihaiensis strain Alg07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1169734..1180774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9C19_RS05510 (A9C19_05380) - 1170896..1171687 (+) 792 WP_072579010.1 PRC-barrel domain-containing protein -
  A9C19_RS05515 (A9C19_05385) - 1171704..1173038 (+) 1335 WP_072579011.1 VanW family protein -
  A9C19_RS05520 (A9C19_05390) - 1173063..1174724 (+) 1662 WP_072579012.1 GspE/PulE family protein -
  A9C19_RS05525 (A9C19_05395) pilT 1174734..1175774 (+) 1041 WP_072579013.1 type IV pilus twitching motility protein PilT Machinery gene
  A9C19_RS05530 (A9C19_05400) - 1175774..1176982 (+) 1209 WP_072579014.1 type II secretion system F family protein -
  A9C19_RS05535 (A9C19_05405) - 1177078..1177545 (+) 468 WP_072579015.1 type II secretion system protein -
  A9C19_RS05540 (A9C19_05410) - 1177616..1178392 (+) 777 WP_072579016.1 prepilin peptidase -
  A9C19_RS05545 (A9C19_05415) pilM 1178408..1179406 (+) 999 WP_072579017.1 type IV pilus biogenesis protein PilM -
  A9C19_RS05550 (A9C19_05420) - 1179412..1179996 (+) 585 WP_145925779.1 hypothetical protein -
  A9C19_RS05555 (A9C19_05425) - 1179993..1180766 (+) 774 WP_072579019.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38142.08 Da        Isoelectric Point: 7.5474

>NTDB_id=185298 A9C19_RS05525 WP_072579013.1 1174734..1175774(+) (pilT) [Bacillus weihaiensis strain Alg07]
MKSKIDTILKFAFEKNASDIHLSVGVPPIMRVNGDLVKIGEDFLMPADTEGMAKAMISQAKWPMFEEKGELDFSYGIPGV
SRFRVNAFRQRSCISLAIRIVPRNIPSMDDLHLPPTLKNIVEKPHGLILVTGPTGSGKSTTLASMINFMNTTMKRHIITL
EDPIEYLHKHQLCVVDQREVGFDTMSFASGLKSALRQDPDVILVGEMRDLETIHTAITAAETGHLVLGTLHTSSAASTID
RMIDVFPANQQPQIRLQLASVLVGVISQRLFPTIDRSGRRAVIEILLNNSAVSSLIRNEKTYQIPSVIQTSRAQGMQSFE
SSLSEYVKAGVIASEAALPYQQRASE

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=185298 A9C19_RS05525 WP_072579013.1 1174734..1175774(+) (pilT) [Bacillus weihaiensis strain Alg07]
ATGAAATCAAAAATTGATACTATTTTAAAGTTTGCTTTTGAAAAGAATGCATCTGACATTCACCTATCCGTCGGAGTTCC
ACCGATTATGAGGGTGAATGGAGACCTAGTTAAAATCGGCGAAGATTTCTTAATGCCAGCAGATACAGAAGGAATGGCAA
AAGCAATGATTTCTCAGGCAAAATGGCCAATGTTCGAAGAAAAAGGGGAGCTTGATTTCTCTTATGGTATTCCGGGGGTT
TCTCGCTTTCGAGTAAATGCCTTTCGTCAACGCTCGTGCATTTCCCTTGCCATACGTATTGTTCCGAGAAACATACCTTC
AATGGATGATTTGCATTTACCACCAACCTTAAAAAATATCGTAGAAAAGCCCCATGGACTTATTTTGGTAACAGGTCCAA
CGGGAAGTGGTAAATCAACGACATTAGCGTCTATGATTAACTTCATGAACACGACAATGAAACGCCATATTATTACGTTA
GAGGACCCTATTGAGTATTTGCACAAGCATCAATTATGTGTCGTTGATCAACGTGAGGTTGGTTTTGATACAATGAGCTT
TGCATCAGGCTTAAAATCAGCACTCAGACAGGATCCAGATGTCATACTAGTAGGTGAAATGCGTGATCTAGAAACAATCC
ATACAGCGATTACAGCTGCTGAGACAGGACATTTAGTGCTTGGAACCTTACACACGTCCTCAGCTGCATCAACAATTGAT
CGGATGATTGATGTATTTCCAGCTAATCAGCAGCCACAAATTCGATTGCAGCTTGCATCTGTTCTTGTTGGTGTCATTTC
ACAAAGACTCTTCCCTACGATTGATCGATCAGGACGTCGAGCTGTAATTGAAATCCTTTTAAATAATTCAGCTGTGTCAA
GCTTAATACGAAATGAAAAAACGTATCAAATTCCTAGTGTGATACAAACGAGTCGCGCACAAGGGATGCAATCGTTTGAA
AGCAGCTTATCTGAATACGTTAAAGCAGGAGTAATTGCTTCTGAAGCTGCATTACCTTATCAGCAAAGGGCGTCTGAATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L3MPE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.882

98.266

0.5
  pilT Legionella pneumophila strain Lp02

47.929

97.688

0.468
  pilT Neisseria gonorrhoeae MS11

48.503

96.532

0.468
  pilT Legionella pneumophila strain ERS1305867

47.929

97.688

0.468
  pilT Pseudomonas stutzeri DSM 10701

48.071

97.399

0.468
  pilT Neisseria meningitidis 8013

48.204

96.532

0.465
  pilT Pseudomonas aeruginosa PAK

48.048

96.243

0.462
  pilT Vibrio cholerae strain A1552

48.338

95.665

0.462
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.338

95.665

0.462
  pilT Acinetobacter baumannii D1279779

47.748

96.243

0.46
  pilT Acinetobacter baumannii strain A118

47.748

96.243

0.46
  pilT Acinetobacter nosocomialis M2

47.748

96.243

0.46
  pilT Acinetobacter baylyi ADP1

45.732

94.798

0.434
  pilU Pseudomonas stutzeri DSM 10701

40.816

99.133

0.405
  pilU Acinetobacter baylyi ADP1

40.653

97.399

0.396
  pilU Vibrio cholerae strain A1552

40.994

93.064

0.382

Multiple sequence alignment