Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A8L59_RS24925 Genome accession   NZ_CP015852
Coordinates   5556041..5557075 (-) Length   344 a.a.
NCBI ID   WP_003229114.1    Uniprot ID   A0A031GC41
Organism   Pseudomonas koreensis strain CRS05-R5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5551041..5562075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8L59_RS24900 (A8L59_24870) - 5551505..5552776 (+) 1272 WP_064589637.1 dihydroorotase -
  A8L59_RS24905 (A8L59_24875) - 5553099..5554085 (+) 987 WP_064589638.1 lipid II-degrading bacteriocin -
  A8L59_RS24910 (A8L59_24880) - 5554126..5554530 (+) 405 WP_064589639.1 hypothetical protein -
  A8L59_RS24915 (A8L59_24885) - 5554608..5555042 (-) 435 WP_064589640.1 NINE protein -
  A8L59_RS24920 (A8L59_24890) - 5555291..5555917 (+) 627 WP_064589641.1 C40 family peptidase -
  A8L59_RS24925 (A8L59_24895) pilT 5556041..5557075 (-) 1035 WP_003229114.1 type IV pilus twitching motility protein PilT Machinery gene
  A8L59_RS24930 (A8L59_24900) - 5557132..5557818 (+) 687 WP_064589642.1 YggS family pyridoxal phosphate-dependent enzyme -
  A8L59_RS24935 (A8L59_24905) proC 5557852..5558670 (+) 819 WP_064589643.1 pyrroline-5-carboxylate reductase -
  A8L59_RS24940 (A8L59_24910) - 5558681..5559271 (+) 591 WP_064589644.1 YggT family protein -
  A8L59_RS24945 (A8L59_24915) - 5559268..5559558 (+) 291 WP_064589645.1 DUF167 domain-containing protein -
  A8L59_RS24950 (A8L59_24920) metX 5559791..5560930 (+) 1140 WP_064589646.1 homoserine O-succinyltransferase MetX -
  A8L59_RS24955 (A8L59_24925) metW 5560938..5561558 (+) 621 WP_064589647.1 methionine biosynthesis protein MetW -
  A8L59_RS24960 (A8L59_24930) - 5561585..5562019 (+) 435 WP_064589648.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0158

>NTDB_id=182514 A8L59_RS24925 WP_003229114.1 5556041..5557075(-) (pilT) [Pseudomonas koreensis strain CRS05-R5]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KELVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=182514 A8L59_RS24925 WP_003229114.1 5556041..5557075(-) (pilT) [Pseudomonas koreensis strain CRS05-R5]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGAATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACACCTGGAAACGGATTTCTCGTTCGAAGTCCCCGGGGTGGCGCGC
TTTCGGGTCAATGCGTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGAAAGTGCTGAGCATGGA
AGACCTCGGCATGGGCGATGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGATTACCTCAACACCCATCGCCATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTTCACGAATCGCGCAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCGCAGCTTCGCCAC
CGCCCTGCGTTCAGCACTTCGCGAAGACCCGGACGTGATTCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCTGCCGAAACCGGGCATTTGGTGTTCGGCACCCTGCACACCACGTCAGCGGCGAAAACCATCGACCGGGTG
GTGGATGTGTTTCCGGGGGATGAGAAATCCATGGTGCGTTCGATGTTGTCCGAGTCACTGCTGGCCGTGGTCTCGCAGAC
TCTGATCAAGAAAATCGGCGGTGGGCGGGTGGCGGCGCACGAGATCATGCTGGGAACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAAATGTATTCGGCGATTCAGACCGGTGGGTCGTTGGGGATGCAGACACTCGACATGTGCCTG
AAGGAGCTGGTGACCAAGGGTTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A031GC41

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment