Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A7983_RS03125 Genome accession   NZ_CP015750
Coordinates   689456..690472 (-) Length   338 a.a.
NCBI ID   WP_005974835.1    Uniprot ID   A0AAW3ELA7
Organism   Pectobacterium wasabiae CFBP 3304     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 684456..695472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7983_RS03110 (A7983_03110) nsrR 684861..685286 (-) 426 WP_005974824.1 nitric oxide-sensing transcriptional repressor NsrR -
  A7983_RS03115 (A7983_03115) - 685575..687146 (+) 1572 Protein_610 methyl-accepting chemotaxis protein -
  A7983_RS03120 (A7983_03120) - 687655..689487 (+) 1833 WP_005974832.1 diguanylate cyclase -
  A7983_RS03125 (A7983_03125) pilT 689456..690472 (-) 1017 WP_005974835.1 type IV pilus twitching motility protein PilT Machinery gene
  A7983_RS03130 (A7983_03130) - 690494..691207 (+) 714 WP_005974838.1 YggS family pyridoxal phosphate-dependent enzyme -
  A7983_RS03135 (A7983_03135) proC 691284..692105 (+) 822 WP_005974842.1 pyrroline-5-carboxylate reductase -
  A7983_RS03140 (A7983_03140) - 692155..692724 (+) 570 WP_005974844.1 YggT family protein -
  A7983_RS03145 (A7983_03145) yggU 692721..693011 (+) 291 WP_005974847.1 DUF167 family protein YggU -
  A7983_RS03150 (A7983_03150) rdgB 693029..693622 (+) 594 WP_005974849.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  A7983_RS03155 (A7983_03155) hemW 693615..694754 (+) 1140 WP_005974851.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36959.32 Da        Isoelectric Point: 6.5702

>NTDB_id=181925 A7983_RS03125 WP_005974835.1 689456..690472(-) (pilT) [Pectobacterium wasabiae CFBP 3304]
MELDEWVARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPVQREQLRQIGQVDGALMLPDGQ
RLRVNVFHQREGLSVALRIIPFVQPSLDELQAPPVLATLLEKPNGLILITGATGSGKSTTLAAMVGALNDSSDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSVSFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRRASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLMQTGAAAGMQTFEQS
DQQRRRDGLISHSCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=181925 A7983_RS03125 WP_005974835.1 689456..690472(-) (pilT) [Pectobacterium wasabiae CFBP 3304]
ATGGAATTGGATGAGTGGGTGGCGCGTAGTGTAAAACATAATGCCTCTGATCTGCACCTTTGTAGCGGGCACCCTCCAGT
GTTACGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAACAGGTGGCACAATGGTGTGATG
CCTGGCTTGAACCGGTTCAGAGAGAGCAATTGCGGCAGATTGGGCAAGTGGACGGCGCGTTAATGTTGCCTGATGGGCAA
CGCCTGCGGGTAAATGTGTTTCACCAGCGGGAAGGGCTGTCTGTCGCGCTGCGTATTATCCCGTTTGTTCAACCTTCATT
GGATGAATTACAGGCCCCACCCGTCCTTGCGACGCTGCTGGAGAAACCAAATGGGCTGATCTTGATTACCGGTGCGACGG
GCAGCGGTAAATCTACGACGCTGGCGGCAATGGTCGGGGCGTTAAATGACAGCAGCGATCGCCACGTGATCACGTTGGAA
GATCCGATCGAATTTATTCATACCAGCCGACGCTGCCTGATCCAACAGCGCGAGATCGGTGCACACAGCGTGTCTTTTGC
GCAGGCGCTACGGGCGGCATTAAGGGAGGATCCCGATGTGATTCTGCTGGGCGAACTGCGCGATACGGAGACGATTCGGT
TGGCGTTGACCGCGGCGGAAACCGGCCATCTGGTACTGTCAACGCTGCATACACGTCGTGCATCGCAGGCTGTTGATCGG
TTGATTGATGTCTTCCCCGGTGAAGAGAAAGCGTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCCGTGGTGACGCA
AAAACTGTTGCCTGCAGCGCAAGGTGGCCGAATCGCGCTGTATGAAGTGCTGACGGCCACCGCGGCAGTCAGCAACCTGA
TTCGTGAGGGGAAAACGCACCAGCTTCCCGGCCTGATGCAAACCGGAGCTGCTGCGGGAATGCAAACATTCGAGCAAAGC
GACCAGCAGCGCCGCCGGGATGGGCTTATTTCACACAGTTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

99.408

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.703

95.562

0.494
  pilT Vibrio cholerae strain A1552

51.703

95.562

0.494
  pilT Neisseria gonorrhoeae MS11

49.702

99.408

0.494
  pilT Acinetobacter baylyi ADP1

47.633

100

0.476
  pilT Legionella pneumophila strain ERS1305867

47.463

99.112

0.47
  pilT Pseudomonas stutzeri DSM 10701

47.463

99.112

0.47
  pilT Pseudomonas aeruginosa PAK

47.463

99.112

0.47
  pilT Legionella pneumophila strain Lp02

47.463

99.112

0.47
  pilT Acinetobacter nosocomialis M2

46.866

99.112

0.465
  pilT Acinetobacter baumannii D1279779

46.866

99.112

0.465
  pilT Acinetobacter baumannii strain A118

46.866

99.112

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.598

97.041

0.423
  pilU Pseudomonas stutzeri DSM 10701

40.653

99.704

0.405
  pilU Vibrio cholerae strain A1552

39.169

99.704

0.391
  pilU Acinetobacter baylyi ADP1

38.82

95.266

0.37

Multiple sequence alignment