Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PS273GM_RS02675 Genome accession   NZ_CP015641
Coordinates   594324..594740 (+) Length   138 a.a.
NCBI ID   WP_420480417.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain 273     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589324..599740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS273GM_RS02660 (PS273GM_02660) pilD 590296..591162 (-) 867 WP_045424777.1 prepilin peptidase Machinery gene
  PS273GM_RS02665 (PS273GM_02665) pilC 591165..592382 (-) 1218 WP_045424774.1 type II secretion system F family protein Machinery gene
  PS273GM_RS02670 (PS273GM_02670) pilB 592384..594087 (-) 1704 WP_064480512.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PS273GM_RS02675 (PS273GM_02675) pilA/pilAI 594324..594740 (+) 417 WP_420480417.1 pilin Machinery gene
  PS273GM_RS02680 (PS273GM_02680) pilA/pilAII 594782..595234 (+) 453 WP_335743695.1 pilin Regulator
  PS273GM_RS02685 (PS273GM_02685) - 595269..596651 (+) 1383 WP_231107999.1 O-antigen ligase family protein -
  PS273GM_RS02690 (PS273GM_02690) tfpZ 596660..597397 (+) 738 WP_045431604.1 TfpX/TfpZ family type IV pilin accessory protein -
  PS273GM_RS02695 (PS273GM_02695) cysN 597521..599419 (-) 1899 WP_045424763.1 sulfate adenylyltransferase subunit CysN -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14004.00 Da        Isoelectric Point: 8.0987

>NTDB_id=181639 PS273GM_RS02675 WP_420480417.1 594324..594740(+) (pilA/pilAI) [Stutzerimonas stutzeri strain 273]
MQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSNAAAALAEITPGKIGFEQAVNEGKTPSTTATDPGYIGVGATTSYC
GVAVTFTQATGVGSIACTTTGGNAGKFNSKAITLNRTAEGAWSCASTLDAKYKPGKCS

Nucleotide


Download         Length: 417 bp        

>NTDB_id=181639 PS273GM_RS02675 WP_420480417.1 594324..594740(+) (pilA/pilAI) [Stutzerimonas stutzeri strain 273]
ATGCAAAAAGGTTTTACCCTGATTGAACTGATGATCGTAGTCGCGATTATCGGGATTTTGGCCGCAATTGCGCTGCCGGC
ATATCAAGACTACACCGTACGCTCCAACGCTGCCGCTGCCCTGGCCGAAATCACCCCTGGCAAGATAGGATTTGAGCAGG
CGGTTAACGAGGGTAAAACCCCGTCGACCACTGCAACCGATCCGGGCTACATCGGCGTTGGCGCTACAACTTCTTATTGT
GGCGTAGCGGTCACTTTCACCCAGGCAACCGGTGTTGGCTCAATCGCTTGTACAACTACTGGCGGCAACGCTGGCAAGTT
CAATAGCAAAGCAATCACGCTTAATCGTACCGCCGAGGGTGCATGGTCTTGCGCCAGCACCTTGGACGCCAAGTACAAGC
CAGGCAAGTGCTCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(33-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

79.71

100

0.797
  pilA/pilAII Pseudomonas stutzeri DSM 10701

64.444

97.826

0.63
  pilA Pseudomonas aeruginosa PAK

47.945

100

0.507
  pilA Acinetobacter baumannii strain A118

46.763

100

0.471
  comP Acinetobacter baylyi ADP1

44.138

100

0.464
  pilA2 Legionella pneumophila str. Paris

41.781

100

0.442
  pilA2 Legionella pneumophila strain ERS1305867

41.781

100

0.442
  pilA Vibrio cholerae strain A1552

42.657

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.657

100

0.442
  pilA Vibrio cholerae C6706

42.657

100

0.442
  pilA Ralstonia pseudosolanacearum GMI1000

37.58

100

0.428
  pilE Neisseria gonorrhoeae strain FA1090

40.31

93.478

0.377
  comF Acinetobacter baylyi ADP1

36.429

100

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

35.971

100

0.362

Multiple sequence alignment