Detailed information NTG0181636

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PS273GM_RS02660
• Genome accession: NZ_CP015641
• Coordinates: 590296..591162 (-)
• Length: 288 a.a.
• NCBI ID: WP_045424777.1
• Uniprot ID: A0A172WKY5
• Organism: Stutzerimonas stutzeri strain 273
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 585296..596162

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PS273GM_RS02630 (PS273GM_02630)	rpsI	586024..586416 (-)	393	WP_021209313.1	30S ribosomal protein S9	-
PS273GM_RS02635 (PS273GM_02635)	rplM	586431..586859 (-)	429	WP_019342644.1	50S ribosomal protein L13	-
PS273GM_RS02640 (PS273GM_02640)	-	587257..588294 (+)	1038	WP_064480510.1	NADP(H)-dependent aldo-keto reductase	-
PS273GM_RS02645 (PS273GM_02645)	-	588385..589077 (+)	693	WP_064480511.1	energy-coupling factor ABC transporter permease	-
PS273GM_RS02650 (PS273GM_02650)	yacG	589127..589324 (-)	198	WP_045424782.1	DNA gyrase inhibitor YacG	-
PS273GM_RS02655 (PS273GM_02655)	coaE	589321..589929 (-)	609	WP_045424780.1	dephospho-CoA kinase	-
PS273GM_RS02660 (PS273GM_02660)	pilD	590296..591162 (-)	867	WP_045424777.1	prepilin peptidase	Machinery gene
PS273GM_RS02665 (PS273GM_02665)	pilC	591165..592382 (-)	1218	WP_045424774.1	type II secretion system F family protein	Machinery gene
PS273GM_RS02670 (PS273GM_02670)	pilB	592384..594087 (-)	1704	WP_064480512.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PS273GM_RS02675 (PS273GM_02675)	pilA/pilAI	594324..594740 (+)	417	WP_420480417.1	pilin	Machinery gene
PS273GM_RS02680 (PS273GM_02680)	pilA/pilAII	594782..595234 (+)	453	WP_335743695.1	pilin	Regulator

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31858.74 Da
• Isoelectric Point: 6.9290

>NTDB_id=181636 PS273GM_RS02660 WP_045424777.1 590296..591162(-) (pilD) [Stutzerimonas stutzeri strain 273]
MSVVTFLASNTLAFVLSALVLGLLVGSFLNVVIHRLPVMMQRDWRAQAREYLDLPIEPTSTFNLVLPNSHCPQCDHEIRP
WENIPLVSWLALRGKCSSCRAPISSRYPLVELLCGFLSAYVASHFGFSWQAGAMLVLTWGLLAMSMIDVDHQLLPDSLVL
PLLWLGLIVNYFGLFAPLPDAMWGAVAGYLCLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVGA
VLGTIMLRMQRADSGTTIPFGPYLAIAGWVALLWGDQITSAYLQFARF

Nucleotide

• Length: 867 bp

>NTDB_id=181636 PS273GM_RS02660 WP_045424777.1 590296..591162(-) (pilD) [Stutzerimonas stutzeri strain 273]
ATGAGTGTCGTTACCTTTTTGGCCAGCAACACGTTGGCCTTTGTTTTAAGCGCGCTGGTGCTAGGCCTGCTAGTGGGCAG
TTTTCTCAACGTGGTTATCCACCGTCTTCCGGTCATGATGCAGCGGGACTGGCGTGCTCAGGCGCGTGAATATCTTGATT
TGCCGATCGAGCCGACTTCGACCTTCAATTTGGTTCTGCCTAACTCTCACTGCCCGCAGTGCGATCATGAAATACGTCCT
TGGGAAAATATTCCCTTGGTCAGCTGGCTAGCGCTGCGCGGTAAGTGCTCGTCGTGCCGCGCGCCCATCAGCAGTCGTTA
CCCCTTGGTCGAGTTGCTCTGCGGGTTCTTGTCCGCCTATGTGGCCTCGCATTTCGGCTTCTCCTGGCAGGCTGGTGCAA
TGCTGGTGCTGACTTGGGGCCTGCTGGCGATGAGCATGATCGATGTCGATCATCAGTTGCTGCCTGACTCCCTTGTGCTG
CCTTTGCTTTGGCTGGGCTTGATCGTCAACTATTTCGGTTTGTTTGCGCCTTTACCCGATGCGATGTGGGGGGCGGTTGC
TGGATATCTTTGCCTGTGGTCGGTGTACTGGCTGTTCAAACTCGTGACCGGCAAGGAGGGCATGGGCTATGGAGACTTCA
AGCTGCTGGCGATGCTGGGGGCTTGGGGTGGATGGCAAGTTTTGCCGCTGACTATTCTTCTCTCTTCGGTCGTGGGCGCG
GTGTTAGGCACCATCATGCTGCGCATGCAAAGAGCGGACAGCGGCACGACCATTCCCTTTGGTCCGTACCTGGCGATTGC
CGGCTGGGTCGCGCTGCTCTGGGGTGACCAGATCACTTCGGCCTATCTGCAATTTGCCAGGTTCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

3D structure

• AlphaFold DB: A0A172WKY5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.246	95.833	0.549
pilD	Vibrio campbellii strain DS40M4	56.777	94.792	0.538
pilD	Acinetobacter nosocomialis M2	50	98.611	0.493
pilD	Acinetobacter baumannii D1279779	49.648	98.611	0.49
pilD	Neisseria gonorrhoeae MS11	52.471	91.319	0.479