Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PS273GM_RS02670 Genome accession   NZ_CP015641
Coordinates   592384..594087 (-) Length   567 a.a.
NCBI ID   WP_064480512.1    Uniprot ID   A0A172WLF9
Organism   Stutzerimonas stutzeri strain 273     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 587384..599087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS273GM_RS02645 (PS273GM_02645) - 588385..589077 (+) 693 WP_064480511.1 energy-coupling factor ABC transporter permease -
  PS273GM_RS02650 (PS273GM_02650) yacG 589127..589324 (-) 198 WP_045424782.1 DNA gyrase inhibitor YacG -
  PS273GM_RS02655 (PS273GM_02655) coaE 589321..589929 (-) 609 WP_045424780.1 dephospho-CoA kinase -
  PS273GM_RS02660 (PS273GM_02660) pilD 590296..591162 (-) 867 WP_045424777.1 prepilin peptidase Machinery gene
  PS273GM_RS02665 (PS273GM_02665) pilC 591165..592382 (-) 1218 WP_045424774.1 type II secretion system F family protein Machinery gene
  PS273GM_RS02670 (PS273GM_02670) pilB 592384..594087 (-) 1704 WP_064480512.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PS273GM_RS02675 (PS273GM_02675) pilA/pilAI 594324..594740 (+) 417 WP_420480417.1 pilin Machinery gene
  PS273GM_RS02680 (PS273GM_02680) pilA/pilAII 594782..595234 (+) 453 WP_335743695.1 pilin Regulator
  PS273GM_RS02685 (PS273GM_02685) - 595269..596651 (+) 1383 WP_231107999.1 O-antigen ligase family protein -
  PS273GM_RS02690 (PS273GM_02690) tfpZ 596660..597397 (+) 738 WP_045431604.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62168.18 Da        Isoelectric Point: 5.3383

>NTDB_id=181638 PS273GM_RS02670 WP_064480512.1 592384..594087(-) (pilB) [Stutzerimonas stutzeri strain 273]
MTDNIALSGLAKQMVLNGLLDDKTAQQAQQQSARNKLSLVTYVVQNKLANGRAVAELAAEQFGVAYMDLNALDKEIQLRD
VVSEKLARQRRVLPLIKRGHKLFVAVSDPTNQQAVTDIQFSTGLTTEAILVEDDKLGLALDKFFESATSGLEDLGDVDLD
GVDVQTVSDDSANDDAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKVFRVRLRTDGILHEVARPPVQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKQLYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPRESLLEEGFP
ADRIGTFKIYGPIGCENCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=181638 PS273GM_RS02670 WP_064480512.1 592384..594087(-) (pilB) [Stutzerimonas stutzeri strain 273]
ATGACCGACAACATTGCCCTTTCCGGCCTGGCCAAGCAGATGGTGCTGAACGGCCTGCTCGACGACAAAACTGCACAGCA
AGCGCAACAGCAGTCGGCGCGTAACAAGCTGTCGCTGGTGACCTATGTCGTGCAGAACAAGCTTGCCAATGGCCGGGCGG
TCGCTGAACTGGCAGCTGAGCAGTTTGGTGTCGCATACATGGACCTCAATGCGCTGGACAAGGAGATTCAATTAAGGGAC
GTTGTGTCTGAAAAGTTGGCGCGCCAGCGTCGCGTGTTGCCGCTAATAAAGCGCGGCCACAAGCTATTCGTGGCGGTATC
GGATCCGACCAATCAGCAGGCCGTAACAGACATTCAGTTCAGCACCGGACTGACGACCGAGGCGATCCTGGTCGAGGACG
ACAAGCTTGGGCTGGCCCTCGACAAGTTTTTCGAAAGCGCAACCTCCGGGCTGGAGGATCTCGGTGATGTCGACTTGGAC
GGGGTCGATGTTCAGACCGTGTCGGATGATTCTGCAAATGATGACGCGGGCGAGGCCGCTGACGACGCCCCGGTCGTGCG
CTTCGTCAATAAGATGCTGCTCGACGCCATTCGTGGAGGCTCGTCCGATCTGCACTTTGAACCTTACGAAAAGGTGTTTC
GCGTACGCCTGCGTACGGATGGCATCCTGCATGAGGTGGCGCGGCCACCGGTTCAACTCGCGCCCCGTATCTCCGCACGA
TTGAAAGTCATGGCTGGCCTGGACATCTCCGAGCGGCGCAAGCCGCAGGACGGTCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCCATCGACTTCCGCGTGAACACGCTGCCGACCTTGTGGGGCGAAAAGATCGTGATGCGGATTCTCGACCCCTCCA
GTGCCCAAATGGGCATCGATGCGCTGGGATACGAAGAAGACCAGAAGCAGCTGTATATGAATGCCCTCAAGCAGCCGCAG
GGCATGATTCTGGTCACCGGCCCGACGGGTTCAGGCAAGACGGTATCGCTTTACACCGGGCTGAACATTCTCAATACGGT
GGATGTGAATATATCTACCGCCGAAGATCCGGTGGAAATCAATCTTGAAGGTATCAACCAGGTTAACGTCAATCCTCGTC
AGGGCATGGATTTCTCCCAGGCGCTGCGGGCGTTTCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGAT
CTTGAAACAGCCGAGATCGCGATCAAGGCGGCGCAAACCGGGCACATGGTGATGTCGACCTTGCACACCAACAGCGCCGC
CGAAACCCTGACGCGTCTACGCAATATGGGCGTGGCGGCCTTCAACATCGCTACCTCGGTGAACCTGATCATTGCCCAGC
GCCTGGCGCGCAAGCTTTGCAACAGCTGCAAGAAAGAAGTGGAGCTGCCCCGCGAATCCCTACTGGAAGAGGGCTTCCCT
GCCGATAGGATCGGAACCTTCAAGATTTACGGCCCGATAGGCTGTGAAAATTGCAAAGGCGGATATAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCACTAGCCAGGATTATCATGGAAGACGGCAACTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAATGACCTGCGTACCTCGGCTCTCGTAAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172WLF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.938

99.647

0.587
  pilB Acinetobacter baylyi ADP1

58.865

99.471

0.586
  pilB Legionella pneumophila strain ERS1305867

54.895

100

0.554
  pilB Vibrio cholerae strain A1552

50.449

98.236

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

48.427

100

0.489
  pilF Neisseria gonorrhoeae MS11

49.201

99.295

0.489
  pilB Vibrio campbellii strain DS40M4

47.544

100

0.478
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.272

100

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

91.534

0.363
  pilF Thermus thermophilus HB27

36.283

99.647

0.362

Multiple sequence alignment