Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GFC29_RS09320 Genome accession   NZ_CP015436
Coordinates   1931172..1932215 (-) Length   347 a.a.
NCBI ID   WP_044742922.1    Uniprot ID   -
Organism   Anoxybacillus sp. B7M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1926172..1937215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFC29_RS09290 (GFC29_1909) gspM 1926218..1926985 (-) 768 WP_044742916.1 type II secretion system protein GspM -
  GFC29_RS09295 (GFC29_1910) - 1926982..1927605 (-) 624 WP_044742917.1 PilN domain-containing protein -
  GFC29_RS09300 (GFC29_1911) pilM 1927589..1928557 (-) 969 WP_044742918.1 type IV pilus biogenesis protein PilM -
  GFC29_RS09305 (GFC29_1912) - 1928575..1929333 (-) 759 WP_044742919.1 A24 family peptidase -
  GFC29_RS09310 (GFC29_1913) - 1929355..1929870 (-) 516 WP_044742920.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GFC29_RS09315 (GFC29_1914) - 1929959..1931170 (-) 1212 WP_044742921.1 type II secretion system F family protein -
  GFC29_RS09320 (GFC29_1915) pilT 1931172..1932215 (-) 1044 WP_044742922.1 type IV pilus twitching motility protein PilT Machinery gene
  GFC29_RS09325 (GFC29_1916) - 1932227..1933891 (-) 1665 WP_044742923.1 GspE/PulE family protein -
  GFC29_RS09330 (GFC29_1917) - 1933903..1935222 (-) 1320 WP_044742924.1 VanW family protein -
  GFC29_RS09335 (GFC29_1918) - 1935242..1936009 (-) 768 WP_044742925.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38348.42 Da        Isoelectric Point: 8.9691

>NTDB_id=179723 GFC29_RS09320 WP_044742922.1 1931172..1932215(-) (pilT) [Anoxybacillus sp. B7M1]
MKQRLDRLLRTAFEAKASDVHLTVGVPPIIRINGELKRYGQEALRPEDTEGIAKAIISETLWPRFEEQGELDFSYGIPGV
SRFRVNVFKQRSCISLAIRIIPTRIPTIDELKLPEVLKQVAAKPQGLVLVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYLHKHGGCIIDQREVGFDTNSFANGLRAALRQDPDVILVGEMRDLETIHTAITAAETGHLVLGTLHTSNAPATID
RIIDVFPPAQQAQIRIQLASVLVAVISQRLFPNVQKTGRVAVTEVLLNSAAVANLIRNEKVHQIVNVMQTSRALGMHTLA
ASIRERLQAGMIAKEAAEPYLREERGE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=179723 GFC29_RS09320 WP_044742922.1 1931172..1932215(-) (pilT) [Anoxybacillus sp. B7M1]
ATGAAGCAACGATTAGACCGTTTATTGCGGACGGCTTTTGAAGCGAAGGCCTCCGATGTTCATTTAACGGTGGGCGTTCC
ACCCATTATACGCATAAATGGAGAGTTAAAGCGATACGGACAGGAAGCGCTGCGTCCCGAGGATACGGAAGGAATAGCGA
AAGCGATCATTTCCGAAACATTATGGCCGCGGTTTGAAGAACAAGGGGAGTTGGATTTTTCTTATGGAATTCCCGGGGTT
TCCCGCTTTCGCGTGAATGTATTTAAACAGCGCTCCTGTATTTCGCTGGCGATTCGCATCATTCCAACAAGAATTCCAAC
GATTGATGAATTAAAGCTTCCCGAGGTGCTCAAGCAGGTTGCCGCCAAGCCTCAAGGGCTCGTCCTGGTCACGGGACCGA
CCGGCAGCGGAAAATCGACAACGCTAGCGGCGATGATTGACTACATGAACAAAACGATGAGGAAGCACATTATTACGCTT
GAAGATCCGATTGAATATTTGCATAAGCATGGCGGCTGCATTATTGACCAGCGCGAGGTCGGATTTGATACGAACAGTTT
TGCCAACGGATTGCGCGCAGCGCTGCGCCAAGATCCGGATGTGATTTTAGTCGGCGAAATGCGCGATCTCGAAACGATCC
ACACGGCGATTACCGCAGCGGAAACGGGCCATCTTGTTCTCGGCACGCTTCATACGTCGAATGCTCCGGCGACGATTGAC
CGCATCATCGATGTCTTTCCGCCTGCGCAGCAGGCGCAAATCCGCATTCAGCTTGCGTCCGTTCTAGTGGCAGTGATTTC
ACAGCGTCTGTTTCCGAATGTGCAAAAAACGGGGCGGGTGGCTGTGACAGAAGTGTTGCTGAACAGCGCGGCGGTGGCGA
ATTTAATTCGCAATGAGAAGGTCCACCAAATCGTCAATGTGATGCAGACAAGCCGGGCATTAGGAATGCATACGCTCGCA
GCGAGCATTAGAGAGCGGCTGCAGGCGGGAATGATTGCGAAGGAGGCCGCCGAGCCGTATTTGAGAGAGGAGCGGGGCGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.797

91.066

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.769

93.66

0.476
  pilT Vibrio cholerae strain A1552

50.769

93.66

0.476
  pilT Pseudomonas aeruginosa PAK

50

94.524

0.473
  pilT Pseudomonas stutzeri DSM 10701

49.24

94.813

0.467
  pilT Acinetobacter baumannii D1279779

48.182

95.101

0.458
  pilT Acinetobacter nosocomialis M2

48.182

95.101

0.458
  pilT Acinetobacter baumannii strain A118

48.182

95.101

0.458
  pilT Acinetobacter baylyi ADP1

48.923

93.66

0.458
  pilT Neisseria gonorrhoeae MS11

47.879

95.101

0.455
  pilT Neisseria meningitidis 8013

47.879

95.101

0.455
  pilT Legionella pneumophila strain ERS1305867

47.273

95.101

0.45
  pilT Legionella pneumophila strain Lp02

47.273

95.101

0.45
  pilU Pseudomonas stutzeri DSM 10701

44.745

95.965

0.429
  pilU Vibrio cholerae strain A1552

43.243

95.965

0.415
  pilU Acinetobacter baylyi ADP1

41.875

92.219

0.386

Multiple sequence alignment