Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A0V43_RS11005 Genome accession   NZ_CP014749
Coordinates   2241371..2242426 (-) Length   351 a.a.
NCBI ID   WP_049624187.1    Uniprot ID   A0A142D2T0
Organism   Geobacillus sp. JS12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2236371..2247426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0V43_RS10975 (A0V43_10975) - 2236653..2237354 (-) 702 WP_063193083.1 hypothetical protein -
  A0V43_RS10980 (A0V43_10980) - 2237351..2237926 (-) 576 WP_063193084.1 hypothetical protein -
  A0V43_RS10985 (A0V43_10985) pilM 2237916..2238842 (-) 927 WP_049624191.1 pilus assembly protein PilM -
  A0V43_RS10990 (A0V43_10990) - 2238872..2239617 (-) 746 Protein_2281 A24 family peptidase -
  A0V43_RS10995 (A0V43_10995) - 2239640..2240080 (-) 441 WP_063193085.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A0V43_RS11000 (A0V43_11000) - 2240173..2241384 (-) 1212 WP_049624188.1 type II secretion system F family protein -
  A0V43_RS11005 (A0V43_11005) pilT 2241371..2242426 (-) 1056 WP_049624187.1 type IV pilus twitching motility protein PilT Machinery gene
  A0V43_RS11010 (A0V43_11010) - 2242439..2244103 (-) 1665 WP_020756620.1 ATPase, T2SS/T4P/T4SS family -
  A0V43_RS11015 (A0V43_11015) - 2244100..2245434 (-) 1335 WP_063193086.1 G5 domain-containing protein -
  A0V43_RS11020 (A0V43_11020) - 2245453..2246223 (-) 771 WP_033006646.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38348.35 Da        Isoelectric Point: 8.0936

>NTDB_id=173596 A0V43_RS11005 WP_049624187.1 2241371..2242426(-) (pilT) [Geobacillus sp. JS12]
MKEKLDGLLRAAFEYRASDVHLTVGAPPIFRINGELKGYGQERLRPADTEQMAKAVIPAPLWEQFQQTGELDFSYGIAGV
SRFRINVFKQRSCVSLAVRLIPPRIPTLEELGLPSVLKRIAEKPYGLVLVTGPTGSGKSTTLAAMIDYMNKTMSRHIITL
EDPIEYLHQHGKSIIDQREVGFDTNSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPSAQQAQIRLQLASVLVAVMAQRLFPNAQKKGRTAAIEILLNNAAVANLIRNEKEHQIVNIMQTSKSMGMQTME
MSVKELAAAGRIDPAAAEAYVARERGADGAI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=173596 A0V43_RS11005 WP_049624187.1 2241371..2242426(-) (pilT) [Geobacillus sp. JS12]
ATGAAGGAAAAGCTGGATGGGTTGTTGCGGGCAGCTTTTGAATACCGTGCGTCTGATGTACATTTGACAGTCGGGGCGCC
GCCTATCTTTCGGATCAATGGAGAACTGAAGGGGTATGGGCAAGAACGGCTCAGACCAGCGGATACGGAGCAAATGGCGA
AAGCCGTCATTCCTGCACCGCTCTGGGAGCAGTTTCAACAAACGGGGGAGCTTGATTTTTCATACGGTATTGCGGGTGTT
TCCCGCTTTCGAATCAATGTGTTTAAGCAGCGGTCGTGCGTGTCGCTTGCCGTGCGCCTCATCCCACCGCGCATTCCGAC
ATTAGAAGAGCTTGGCTTGCCAAGCGTCTTAAAGCGCATTGCGGAAAAGCCATACGGACTTGTGCTTGTCACCGGGCCGA
CCGGCAGCGGCAAATCGACGACATTGGCAGCGATGATCGACTATATGAACAAAACGATGTCCAGGCACATCATCACGCTT
GAAGACCCGATTGAATATTTGCATCAGCACGGAAAATCGATCATCGACCAGCGCGAAGTCGGATTTGACACAAACAGCTT
CGCCGCTGGGTTGCGGGCGGCGCTTCGCCAAGATCCAGACGTCATTTTAGTCGGGGAAATGCGCGATTTGGAAACGATCC
AAACGGCTATCACGGCAGCCGAGACGGGCCATTTAGTGCTCGGCACTCTTCATACCGCGAGCGCGCCGGCGGCGATTGAG
CGGATGATTGACGTTTTTCCGTCTGCCCAGCAGGCGCAAATTCGGCTTCAGCTCGCTTCCGTTCTTGTCGCGGTCATGGC
CCAGCGCTTGTTTCCGAACGCTCAAAAGAAAGGGCGGACGGCGGCGATCGAAATTTTGCTTAACAACGCCGCGGTGGCGA
ATTTGATCCGCAATGAAAAAGAGCATCAAATCGTCAATATCATGCAGACGAGCAAAAGCATGGGCATGCAGACGATGGAG
ATGAGCGTCAAAGAGCTCGCCGCCGCCGGGCGCATTGATCCAGCGGCGGCGGAAGCGTATGTGGCAAGGGAGCGTGGGGC
TGATGGCGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A142D2T0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.952

89.174

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.605

95.157

0.453
  pilT Vibrio cholerae strain A1552

47.605

95.157

0.453
  pilT Neisseria gonorrhoeae MS11

47.305

95.157

0.45
  pilT Neisseria meningitidis 8013

47.305

95.157

0.45
  pilT Acinetobacter baumannii strain A118

46.97

94.017

0.442
  pilT Acinetobacter baumannii D1279779

46.97

94.017

0.442
  pilT Acinetobacter baylyi ADP1

46.687

94.587

0.442
  pilT Legionella pneumophila strain ERS1305867

46.667

94.017

0.439
  pilT Legionella pneumophila strain Lp02

46.667

94.017

0.439
  pilT Acinetobacter nosocomialis M2

46.667

94.017

0.439
  pilT Pseudomonas aeruginosa PAK

48.418

90.028

0.436
  pilT Pseudomonas stutzeri DSM 10701

46.584

91.738

0.427
  pilU Vibrio cholerae strain A1552

42.73

96.011

0.41
  pilU Pseudomonas stutzeri DSM 10701

42.857

93.732

0.402

Multiple sequence alignment